PT  - JOURNAL ARTICLE
AU  - Tong, L
AU  - Gao, X
AU  - Feng, L
AU  - Yao, D
AU  - Zhang, X
AU  - Du, Y
AU  - Zhou, Y
AU  - Chen, F
TI  - A novel pigeon paramyxovirus type 1 isolated from a sick racing pigeon in the Qinghai-Tibet Plateau of China shows high virulence in chickens
DP  - 2024 Nov 29
TA  - Veterinární medicína
PG  - 389--394
VI  - 69
IP  - 11
AID - 10.17221/15/2024-VETMED
IS  - 03758427
AB  - Pigeon paramyxovirus type-1 (PPMV-1) is the causative agent of pigeon Newcastle disease (ND), which has caused huge losses to the pigeon industry. In this study, a PPMV-1 strain, PPMV-1/QH-01/CH/23, was isolated from a sick racing pigeon in the Qinghai-Tibet Plateau, China in 2023. The mean death time of chicken embryos and the intracerebral pathogenicity index (ICPI) were 76.8 h and 1.25, indicating a mesogenic strain. Pigeon morbidity and mortality were 100% and 80%, respectively, and both were 80% in chickens; therefore, this isolate was velogenic for both pigeons and chickens. The fusion gene was amplified and sequenced for phylogenetic analysis, and the results indicated that the isolated strain possessed a virulent fusion protein cleavage site motif, <sup>112</sup>R-R-Q-K-R-F<sup>117</sup>, and belonged to genotype XX (former sub-genotype VIc) of class II; this was different from the predominant sub-genotype, VI.2.1.1.2.2, which is prevalent in pigeons. To the best of our knowledge, this is the first report of a novel genotype XX isolate possessing high virulence for both chickens and pigeons in the Qinghai-Tibet Plateau, China.