Veterinární medicína - Latest articles
Results 121 to 143 of 143:
The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model
A Curukoglu, GCA Gungor, G Akan, A Kukner, G Ogutcu, M Kalaycı, M Temizel, FE Ozgencil
Vet Med - Czech, 2023, 68(4):152-163 | DOI: 10.17221/109/2022-VETMED 
This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied...
Efficacy of the intranasal application of azaperone for sedation in weaned piglets
M Svoboda, J Blahova, J Jarkovsky, A Zacharda, S Hajkova, J Vanhara, J Vasek
Vet Med - Czech, 2023, 68(4):145-151 | DOI: 10.17221/21/2023-VETMED
The aim of the study was to compare the efficacy of the intranasal and parenteral administration of azaperone in order to achieve pig sedation. A total of 32 weaned piglets divided into 4 groups (8 piglets in each group) were used. Group A was injected intramuscularly (i.m.) with azaperone (Stresnil®, 40 mg/ml inj.; Elanco Animal Health) at a dose of 2 mg/kg of body weight (b.w.). Group B received a dose of 2 mg/kg b.w. of azaperone intranasally. Group C was given azaperone intranasally at a dose of 4 mg/kg b.w. Group D was given 1 ml of saline intranasally and served...
Citalopram in vitro metabolism in a beagle dog: A role for CYP2D15 in the production of toxic didesmethylcitalopram?
B Rochat, E Paus, C Maitre, P Baumann
Vet Med - Czech, 2023, 68(4):135-144 | DOI: 10.17221/65/2022-VETMED
After administration of the serotonergic antidepressant citalopram (CIT) to beagle dogs, they may experience severe convulsive attacks in relation with considerably higher plasma concentrations of the metabolite didesmethyl-CIT (DDCIT), when compared to those in humans medicated with CIT. This pilot study aimed at determining the role of cytochrome P-450 (CYP450) isozymes in the in vitro metabolism of CIT to desmethyl-CIT (DCIT), and of DCIT to DDCIT in the liver microsomes of a single beagle dog. The incubations with racemic CIT or DCIT reveal a high affinity enzyme with Km between 0.3 µM and 1.4 µM for S- and R-DCIT and S- and R-DDCIT...
Malignant ovarian dysgerminoma in a 16-year-old leopard gecko (Eublepharis macularius)
H Zborilova, J Dietz, KO Heckers, R Dvorakova, Z Knotek, E Cermakova
Vet Med - Czech, 2023, 68(3):129-134 | DOI: 10.17221/107/2022-VETMED
The 16-year-old female leopard gecko (Eublepharis macularius) was presented with distended coelom and cachexia. Examination of the faecal sample ruled out the presence of protozoan parasites. A radiographic examination confirmed the presence of radiopaque foreign material in the intestine. The conservative treatment with tramadol, butylscopolamine, famotidine, vitamin B complex, and supportive fluid therapy with Hartmann solution and Duphalyte, was performed for 14 days. Ultrasonographic examination revealed the presence of a large mass adherent to the liver (with hypoechoic regions), a thin-walled cystic structure close to the liver, and coelomic...
Serum biochemical reference interval determination in wild Siberian weasel (Mustela sibirica)
M Ha, YD Suh, S Ahmed, DN Lee, JH Han, YK Kim, SC Yeon
Vet Med - Czech, 2023, 68(3):122-128 | DOI: 10.17221/102/2022-VETMED
Determining reference intervals (RI) is a valuable asset for assessing the health of wildlife species. This is the first study to establish serum biochemical RIs in Siberian weasels. Forty-two healthy free-ranging Siberian weasels were captured live and brought to Seoul Wildlife Center between June 2021 and August 2022. Blood samples from 42 healthy Siberian weasels of both sexes were used to calculate RIs. An automated analyser was used to perform serum biochemistry profiles. The American Society for Veterinary Clinical Pathology recommendations were used to calculate a nonparametric RI with 90% confidence intervals. The RIs of albumin, total protein,...
The impact of urine storage methods on the results of quantitative bacterial culture in dog and cat
CJ Lien, SL Wang
Vet Med - Czech, 2023, 68(3):116-121 | DOI: 10.17221/111/2022-VETMED
Quantitative bacterial culture (QBC) is the gold standard for determining urinary tract infections. However, the majority of urine samples were sent to a lab for further bacterial culture. Therefore, the storage condition was vital to maintain the quality and accuracy of the urine samples. The main objective of our study was to examine the urine QBC outcomes of (1) immediate culture, (2) culture after urine sample storage at ambient temperature for 24 h, and (3) culture after urine sample storage at 4 °C for 24 hours. There were 49 feline samples and 30 canine samples included in this study. All QBC samples kept at ambient temperature...
Development and application of a low-priced duplex quantitative PCR assay based on SYBR Green I for the simultaneous detection of porcine deltacoronavirus and porcine sapelovirus
SJ Lu, MY Ma, XG Yan, FJ Zhao, WY Hu, QW Ding, HJ Ren, YQ Xiang, LL Zheng
Vet Med - Czech, 2023, 68(3):106-115 | DOI: 10.17221/79/2022-VETMED
Porcine deltacoronavirus (PDCoV) and porcine sapelovirus (PSV) are two viruses that can cause diarrhoea in pigs and bring great economic loss to the pig industry. In this research, a duplex real-time quantitative polymerase chain reaction (qPCR) assay based on SYBR Green І was developed to simultaneously detect PDCoV and PSV. No specific melting peaks were found in other porcine diarrhoea-associated viruses, indicating that the method developed in this study had good specificity. The detection limits of PDCoV and PSV were 1.0 × 101 copies μl–1 and 1.0 × 102 copies μl–1, respectively....
Lingual morphology of domesticated Asian small-clawed otters in Yogyakarta, Indonesia
AK Anjani, GR Saragih, H Wihadmadyatami, DL Kusindarta
Vet Med - Czech, 2023, 68(3):91-105 | DOI: 10.17221/62/2022-VETMED
This study aimed to observe the lingual morphology of the domesticated Asian small-clawed otter, Aonyx cinereus (A. cinereus), from Yogyakarta, Indonesia. Six domesticated A. cinereus adults were obtained from a local otter breeder in Yogyakarta, without regard to gender. The animals were acclimated to the laboratory for one day, followed by macroscopy identification and scanning electron microscopy (SEM) and light microscopy (LM) analysis. Macroscopically, the tongue of domesticated A. cinereus is divided into three parts: the apex, corpus and radix. The apex is the shortest part and can move freely....
Adjuvant therapy with toceranib for hepatocellular carcinoma and cholangiocarcinoma in a Pomeranian
L Choi, JY Choi, HY Yoon, K Bae, KA Yoon, JH Kim
Vet Med - Czech, 2023, 68(2):83-89 | DOI: 10.17221/49/2022-VETMED
A 10-year-old spayed female Pomeranian dog was referred for hepatic mass evaluation. Blood tests revealed mildly elevated alkaline phosphatase activities. Computed tomography revealed a mass with multiple nodules on the right hepatic medial lobe adjacent to the caudal vena cava; histopathology confirmed mixed hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC). Because of incomplete resection, adjuvant therapy was recommended. As tumour cells showed PDGFR-α, c-Kit, and FGFR1 overexpression, the anticancer effect of tyrosine kinase inhibitors was evaluated on the cells; toceranib was the most effective and...
Echocardiographic measurements and cardiac anatomy in healthy Western hognose snakes (Heterodon nasicus)
M Oliveri, C Carnabuci, M Vignoli, S Di Feliciantonio, M Di Feliciantonio, L Della Salda, Z Knotek, M di Tommaso, A Luciani
Vet Med - Czech, 2023, 68(2):75-82 | DOI: 10.17221/63/2022-VETMED
This study aims to describe the most important cardiac structures in the Heterodon nasicus through echocardiography and anatomical dissection. Echocardiographic and echo-Doppler measurements were performed on twenty healthy adult Heterodon nasicus (10.10). The values of the ventricular length, aortic diameter, pulmonary trunk diameter, the mean thickness of the interventricular septum, and thicknesses of the wall of the cavum pulmonale (Cav. P) and cavum arteriosum (Cav. A), were measured. The aortic flow and pulmonary trunk flow were recorded. Two dead specimens (1.1) were dissected. The male’s pulmonary trunk...
Verification of accuracy of qPCR method for intravital diagnostics of Macrorhabdus ornithogaster in avian droppings
L Vrbasova, D Molinkova, P Linhart, Z Knotek
Vet Med - Czech, 2023, 68(2):69-74 | DOI: 10.17221/85/2022-VETMED
The objective of this study was to verify the accuracy of the quantitative PCR (qPCR) method for in vivo detection of Macrorhabdus ornithogaster. A total of 100 faecal and gastric mucosa samples from avian cadavers were investigated, using cytological and qPCR techniques (budgerigars, Fischer’s lovebirds, red-crowned parakeets, scarlet-chested parrots, eastern rosellas, domestic canaries, zebra finches, white Java sparrow). Using qPCR, the probability of detecting positive samples of droppings was significantly higher than in the faecal smear microscopy (P < 0.01). Cytology detected the presence of Macrorhabdus ornithogaster...
Presence of zoonotic black-pigmented periodontal pathogens in the oral microbiota of pet and stray cats
V Ozavci, HTY Dolgun, S Kirkan
Vet Med - Czech, 2023, 68(2):62-68 | DOI: 10.17221/59/2022-VETMED
Black-pigmented bacteria are one of the neglected species to cause periodontal disease in cats, and they are also zoonotic agents that pose an infection risk to humans. In this study, we aimed to determine the presence of Porphyromonas gingivalis, Porphyromonas gulae and Prevotella nigrescens in the oral microbiota of pet and stray cats. Dental swab samples were taken from 25 pet cats and 25 stray cats with symptoms of periodontal disease and then investigated by multiplex polymerase chain reaction using 16S rRNA species-specific primers. As a result of the multiplex PCR analysis, P. gingivalis 3/25 (12%), P. nigrescens...
Thrombocytosis in brachycephalic dogs with brachycephalic obstructive airway syndrome
V Erjavec, A Nemec Svete
Vet Med - Czech, 2023, 68(2):57-61 | DOI: 10.17221/23/2021-VETMED
Brachycephalic dogs are prone to a conformation-related respiratory disorder known as brachycephalic obstructive airway syndrome (BOAS). Due to its systemic consequences, BOAS should be considered a systemic disease. The aim of the present study was to investigate platelet count (PLT), mean platelet volume (MPV), and plateletcrit (PCT) in patients with various grades of BOAS and non-brachycephalic dogs. The latter served as a control group. We included 106 patients with BOAS and 41 non-brachycephalic dogs. According to the severity of the disease, BOAS patients were classified into grade 1 (17 dogs), grade 2 (42 dogs), and grade 3 (47 dogs). Thrombocytosis...
Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review
S Kumar, MS Chaves, AFB da Silva, WG Vale, STR Filho, JC Ferreira-Silva, LM Melo, VJF Freita
Vet Med - Czech, 2023, 68(2):45-56 | DOI: 10.17221/48/2022-VETMED
Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo...
List of volume 67 reviewers
Editorial Department
Vet Med - Czech, 2023, 68(1):I-II
Corrigendum: The effect of brown seaweed and polyphenol supplementation in male rabbits on the blood profile and antioxidant markers
M Massanyi
Vet Med - Czech, 2023, 68(1):43 | DOI: 10.17221/4/2023-VETMED
Fowl adenovirus induced different manifestations of the disease in two consecutive chicken breeding flocks in a poultry hall
M Levkutova, M Levkut, R Herich, V Revajova, V Seman, M Cechova, M Levkut
Vet Med - Czech, 2023, 68(1):38-42 | DOI: 10.17221/27/2022-VETMED
This study investigated an adenovirus infection in two consecutive breeding flocks in the same poultry hall. Thirty-six thousand one-day-old chickens of the ROSS 308 hybrid broiler type were kept together in one hall. The chickens in the first breeding flock during fattening did not show any clinical signs of the disease or increased mortality. Typical clinical signs of the adenovirus infection were seen in the second breeding flock. The signs included: depression, apathy, somnolence, a crouched position with a droopy head, fuzzy feathers, anaemic combs and wattles, sporadic nervous signs, and reduced weight gain. Increased mortality was recorded from...
Apocrine cystomatosis: From the aspect of epithelial-mesenchymal transition
TU Kim, SW Lee, SM Baek, JH Yim, YJ Lee, JH Son, SJ Park, JK Park
Vet Med - Czech, 2023, 68(1):33-37 | DOI: 10.17221/77/2022-VETMED
Apocrine cystomatosis, also called epitrichial sweat gland cystomatosis, is a non-neoplastic condition characterised by multiple dilated cysts of sweat gland origin. Histopathologically, these cysts comprise two layers of cells: an inner layer of glandular epithelial cells and an outer layer of myoepithelial cells. A case of apocrine cystomatosis was admitted to a local hospital. The microscopic investigation revealed that some enlarged cysts showed the transition of glandular epithelial cells into a spindle, mesenchymal cell-like morphology. The epithelial-to-mesenchymal transition (EMT) has long been studied as a pathway for embryogenesis, organ...
Antimicrobial activities of nano-emulsion of virgin coconut oil
DC Widianingrum, H Khasanah, L Purnamasari, ME Krismaputri, SG Hwang
Vet Med - Czech, 2023, 68(1):27-32 | DOI: 10.17221/57/2022-VETMED
This study aimed to determine the nano-emulsion of virgin coconut oil (n-VCO) formula that can produce the best size and zone inhibition of antimicrobial activity. The VCO was formulated with the different percentages of Tween 80 (P1: 24%, P2: 25%, P3: 26%) and sorbitol (P1: 36%, P2: 35%, P3: 34%). The particle size of the n-VCO emulsion was observed under transmission electron microscopy (TEM). The antimicrobial activity test of the n-VCO was determined by a challenge test using Salmonella Typhi (S. Typhi), Staphylococcus aureus (S. aureus), and Escherichia...
Comparison of the effects of Aloe vera gel and coconut oil on the healing of open wounds in rats
O Istek, M Tanrisever, S Kucukler, B Karabulut, A Cevik
Vet Med - Czech, 2023, 68(1):17-26 | DOI: 10.17221/101/2021-VETMED
In this study, the effects of Aloe vera gel and coconut oil on wound healing were investigated and compared in rats. Forty-two Wistar albino rats were used during the experiment, in which they were operated on under general anaesthesia to create two full-thickness open skin wounds (created with a 0.5 cm diameter punch biopsy apparatus) on both back sides of the median line. A total of 42 rats were divided into three groups of 14 animals each to receive the topical application of Aloe vera gel (AV group – n = 14), coconut oil (CO group – n = 14) and cold cream (CONT group – n = 14). The medical applications...
Refractive errors in mixed breed dogs of different ages
A Balicka, J Zwolska, M Szadkowski, A Trbolova, I Balicki
Vet Med - Czech, 2023, 68(1):11-16 | DOI: 10.17221/106/2021-VETMED
The purpose of the study was to evaluate the occurrence and range of refractive errors in dogs of different ages. A total of 99 clinically healthy, mixed-breed mesocephalic dogs were included in the study and divided into three different age groups according to the current human/pet analogy chart: 40 adults (23 males, 17 females, 1–8 years old, 3–70 kg), 21 seniors (14 males, 7 females, 6–11 years old, 7–42 kg), and 38 geriatrics (22 males, 16 females, 8–13 years old, 5–45 kg). All the dogs underwent an ophthalmic examination, including Schirmer tear test, tonometry, biomicroscopy, and ophthalmoscopy. Neither eye...
Palliative care for cancer patients in veterinary medicine
CSF Repetti, JR Rueda, CD Porto, R Prevedello Franco, RJS Girio, FFR Manhoso, IB Costa
Vet Med - Czech, 2023, 68(1):2-10 | DOI: 10.17221/76/2022-VETMED
Neoplasms account for approximately half of the deaths of dogs over ten years of age. This finding, associated with the fact that canine cancer patients will often suffer from the consequences of the systemic spread of the tumour and paraneoplastic syndromes, shows the importance of understanding palliative care by veterinarians and owners. In view of this, this review aims to address palliative care that can be used in veterinary medicine to promote the patient’s well-being and quality of life.
In respect of Professor Dr. Karel Hruska, former Director of the Veterinary Research Institute of the Czech Republic
Md. Mazharul Islam
Vet Med - Czech, 2023, 68(1):1