Vet Med - Czech, 2001, 46(2):35-40 | DOI: 10.17221/7849-VETMED

The use of the computer technology for the evaluation of the strict morphological sperm analysisOriginal Paper

Z. Věžník, O. Matoušková, D. Švecová, A. Zajícová
Veterinary Research Institute, Brno, Czech Republic

A programme for evaluating the strict morphological analysis of sperm was developed. The programme was verified by conducting 552 morphological analyses of bull, stallion, boar and human ejaculates. The method was evaluated by comparing the results obtained by routinely used morphological examinations with the results received by the automatic method SASMO. The following advantages of the programme were demonstrated: it is possible to obtain information on the proportion of normal and pathological spermatozoa, to determine the frequency of the respective characteristics analysed, to express detected alterations per pathological spermatozoon (teratosperm index), to express frequency of respective alterations per total number of spermatozoa examined, to display alterations according to their frequency and differentiate between developmental and acquired alterations. It was proved that using the detailed morphological evaluation of sperm by the survival test, it is possible to get statistically significant information on the prediction of sperm survival. The programme represents an important aid for making the morphological evaluation of sperm quality more objective both in veterinary and human medicine and in all insemination stations and assisted reproduction centres.

Keywords: bull; stallion; boar; human; diagnosis; spermatology; morphology; strict method

Published: February 28, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Věžník Z, Matoušková O, Švecová D, Zajícová A. The use of the computer technology for the evaluation of the strict morphological sperm analysis. Vet Med - Czech. 2001;46(2):35-40. doi: 10.17221/7849-VETMED.
Download citation

References

  1. Boersma A., Braun J. (1999): Computer assisted analysis of sperm morphology in veterinary medicine. Berl. Munch. Tierarztl. Wochenschr., 112, 81–85. Go to PubMed...
  2. Check J.H., Bollendorf A., Press M., Blue T. (1992): Standart sperm morphology as a predictor of male fertility potential. Arch. Androl., 28, 39–41. Go to original source... Go to PubMed...
  3. Eliasson R. (1971): Standards for investigation of human semen? Andrologie, 3, 49–64. Go to original source...
  4. Eliasson R. (1981): Analysis of sperm. In: Burger H., De Kretser D.: The Testis. Raven Press, New York. 381–399.
  5. Gravance C.G., Vishwanath R., Pitt C., Garner D.L., Casey P.J. (1998): Effects of cryopreservation on bull sperm head morphometry. J. Androl., 19, 704–709. Go to original source... Go to PubMed...
  6. Gravance C.G., Garner D.L., Pitt C., Vishwanath R., Sax-Gravance S.K., Casey P.J. (1999): Replicate and technician variation associated with computer aided bull sperm head morphometry analysis (ASMA). Department of Obstetric and Gynaecology, University of Auckland, New Zealand, Int. J. Androl., 22, 77–82. Go to original source... Go to PubMed...
  7. Hancock J.L. (1959): The morphologic characteristics of spermatozoa and fertility. Int. J. Fertil., 4, 347–359.
  8. Hofmann N., Freundl G., Florack M. (1985): Die Formstörungen der Spermatozoen im Sperma und Zervikalschleim als Spiegel testekulärer Erkrankungen. Gynäkologie, 18, 191–197.
  9. Host E., Lindenberg S., Ernst E., Christensen F. (1999): Sperm morphology and IVF: embryo quality in relation to sperm morphology following the WHO and Kruger40s strict criteria. Acta Obstet. Gynecol. Scand., 78, 526–529. Go to original source... Go to PubMed...
  10. Johnson W.H. (1997): The significance to bull fertility of morphologically abnormal sperm. Bull Infertil., 13, 255–270. Go to original source... Go to PubMed...
  11. Johnson K.R., Dewey C.E., Bobo J.K., Kelling C.L., Lunstra D.D. (1998): Prevalence of morphologic defects in spermatozoa from beef bulls. JAVMA, 213, 1468–1471. Go to original source... Go to PubMed...
  12. Jouannet P., Ducot B., Feneux X., Spira A. (1988): Male factors and the likehood of pregnancy in infertile couples. 1.Study of sperm characteristics. Int. J. Androl., 11, 379–394. Go to original source... Go to PubMed...
  13. Matoušková O., Cígler M., Chalupa J., Hruška K. (1992): Manual of the Programme STAT-Plus. Veterinary Research Institute, Brno. 168 pp.
  14. Menkveld R., Kruger T.F. (1995): Advantages of strict (Tygerberg) criteria for evaluation of sperm morphology. Int. J. Androl., 18, 36–42. Go to PubMed...
  15. Ombelet W., Menkvelt R., Kruger T., Steeno O. (1995): Sperm morphology assessment: historical review in relation to fertility. Hum. Reprod. Update, 1, 543–557. Go to original source... Go to PubMed...
  16. Ombelet W., Wouters E., Boels L., Cox A., Janssen M., Spiessens C., Vereecken A., Bosmans E., Steeno O. (1997): Sperm morphology assessment: diagnostic potential and comparative analysis of strict or WHO criteria in a fertile and a subfertile population. Int. J. Androl., 20, 367–372. Go to original source... Go to PubMed...
  17. Steigerwald P., Krause W. (1998): Estimation of sperm morphology using a new CASA system. Andrologia, 30, 23–27. Go to original source... Go to PubMed...
  18. Vìžník Z. (1970): Utilization of morphological Indices for a qualitative evaluation of ejaculates (in Czech). Vet. Med. (Praha), 15, 571–578.
  19. WHO (1992): Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction. Cambridge University Press, Cambridge.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.