Vet Med - Czech, 2001, 46(4):95-100 | DOI: 10.17221/7866-VETMED

Psychrobacter immobilis isolated from foods: characteristics and identification

Z. Páčová, E. Urbanová, E. Durnová
1 Masaryk University, Faculty of Science, Czech Collection of Microorganisms, Brno, Czech Republic
2 Èaderský-Envitek Ltd., Brno, Czech Republic
3 Regional Hygienic Station, Ostrava, Czech Republic

A total of 15 strains of Psychrobacter immobilis isolated from animal sources, e.g. cheese, fish and poultry, were tested. A commercial diagnostic kit NEFERMtest 24, conventional tests and determination of fatty acids were used for identification. By using the results of NEFERMtest 24 and numerical identification system TNW version 6.0 the identification was successful on the species level (46.7%) while the correct species identification by using conventional tests increased up to 86.7%. All 9 saccharolytic strains including 7 Czech isolates were identified in most cases on an excellent or very good level by both methods based on biochemical reactions. On the other hand, the identification of 6 asaccharolytic strains was unsuccessful especially by NEFERMtest 24. While 4 asaccharolytic strains were identified correctly on the basis of conventional tests on species or genus level, incorrect identification on species level, for example Ralstonia paucula, Comamonas terrigena, Oligella ureolytica, Moraxella lincolnii andPsychrobacter phenylpyruvicus, was found by using NEFERMtest 24. Determination of fatty acid composition by MIDI System confirmed the species identification of 9 out of the 10 tested strains of P. immobilis and 1 tested strain of Psychrobacter sp.

Keywords: Psychrobacter immobilis; characteristics; identification; foods

Published: April 30, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Páčová Z, Urbanová E, Durnová E. Psychrobacter immobilis isolated from foods: characteristics and identification. Vet Med - Czech. 2001;46(4):95-100. doi: 10.17221/7866-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abel K., de Schmertzing H., Peterson J.I. (1963): Classification of microorganisms by analysis of chemical composition. 1. Feasibility of utilising gas chromatography. J. Bacteriol., 85, 1039–1044. Go to original source... Go to PubMed...
    2. Barrow G.I., Feltham R.K.A. (eds.) (1993): Cowan and Steels’ Manual for the Identification of Medical Bacteria. Third edition. Cambridge University Press, 192–208. Go to original source...
    3. Birnbaum D., Herwaldt L., Low D.E., Noble M., Pfaller M., Sheretz R., Chow A.W. (1994): Efficacy of microbial identification system for epidemiologic typing of coagulase-negative staphylococci. J. Clin. Microbiol., 32, 2113–2119. Go to original source... Go to PubMed...
    4. Bowman J.P., Cavanagh J., Austin J.J., Sanderson K. (1996): Novel Psychrobacter species from Antarctic ornithogenic soil. Int. J. Syst. Bacteriol., 46, 841–848. Go to original source... Go to PubMed...
    5. Bowman J.P., Nichols D.S., McMeekin T.A. (1997): Psychrobacter glacincola sp. nov., a halotolerant, psychrophilic bacterium isolated from Antarctic sea ice. Syst. Appl. Microbiol., 20, 209–215. Go to original source...
    6. Bøvre K., Henriksen S.D. (1976): Minimal standards for description of new taxa within the genera Moraxella and Acinetobacter: proposal by the Subcommittee on Moraxella and allied bacteria. Int. J. Syst. Bacteriol., 26, 92–96. Go to original source...
    7. Firstenberg-Eden R., Rowley D.B., Shattuck G.E. (1980): Factors affecting inactivation of Moraxella – Acinetobacter cells in an irradiation process. Appl. Environ, Microbiol., 40, 480–485. Go to original source... Go to PubMed...
    8. Gennari M., Tomaselli S., Cotrona V. (1999): The microflora of fresh and spoiled sardines (Sardina pilchardus) caught in Adriatic (Mediterranean) Sea and stored in ice. Food Microbiol., 16, 15–28. Go to original source...
    9. Gini G.A. (1990): Ocular infection caused by Psychrobacter immobilis acquired in the hospital. J. Clin. Microbiol., 28, 400–401. Go to original source... Go to PubMed...
    10. Gonzalez C.J., Santos J.A., Garcia-Lopez M.L., Otero A. (2000): Psychrobacters and related bacteria in fresh water fish. J. Food Protect., 63, 315–321. Go to original source... Go to PubMed...
    11. Juni E., Heym G.A., (1986): Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int. J. Syst. Bacteriol., 36, 388–391. Go to original source...
    12. Lloyd-Puryear M., Wallace D., Baldwin T., Hollis D.G., (1991): Meningitis caused by Psychrobacter immobilis in an infant. J. Clin. Microbiol., 29, 2041–2042. Go to original source... Go to PubMed...
    13. Maruyama A., Honda D., Yamamoto H., Kitamura K., Higashihara T. (2000): Phylogenetic analysis of psychrophilic bacteria isolated from the Japan Trench, including a description of the deep-sea species Psychrobacter pacificensis sp. nov. Int. J. Syst. Evol. Microbiol., 50, 835–846. Go to original source... Go to PubMed...
    14. MIDI, Inc. (September 1999): Sherlock, Microbial Identification System, Operating Manual, version 3. MIDI, Inc., Newark, Del., USA.
    15. Mukwaya G..M., Welch D.F. (1989): Subgrouping of Pseudomonas cepacia by cellular fatty acid composition. J. Clin. Microbiol., 27, 2640–2646. Go to original source... Go to PubMed...
    16. Páèová Z., Kocur M. (1978): Phosphatase activity among aerobic and anaerobic bacteria. Zbl. Bakt. I. Abt. Orig. A, 241, 481–487.
    17. Páèová Z., Kocur M. (1984): New medium for detection of esterase and gelatinase activity. Zbl. Bakt. Hyg. A, 258, 69– 73. Go to original source... Go to PubMed...
    18. Páèová Z., Urbanová E. (1995): Application of NEFERMtest to identification of psychrotrophic bacteria isolated from milk and milk products (in Czech). Vet. Med. – Czech, 40, 217–220.
    19. Páèová Z., Urbanová E. (1998): The first isolation of Psychrobacter immobilis from foods in the Czech Republic. Vet. Med. – Czech, 43, 111–113.
    20. Pin C., Baranyi J. (1998). Predictive models as means to quantify the interactions of spoilage organisms. Int. J. Food Microbiol., 41, 59–72. Go to original source... Go to PubMed...
    21. Prieto R., Garcia-Armesto M.R., Garcia-Lopez M.L., Otero A., Moreno B. (1992): Numerical taxonomy of Gram-negative, nonmotile, nonfermentative bacteria isolated during chilled storage of lamb carcasses. Appl. Environ. Microbiol., 58, 2245–2249. Go to original source... Go to PubMed...
    22. Rossau R., van Landschoot A., Gillis M., De Ley J. (1991): Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and related organisms. Int. J. Syst. Bacteriol., 41, 310–319. Go to original source...
    23. Shaw B.G., Latty J.B. (1988): A numerical taxonomic study of non-motile non-fermentative Gram-negative bacteria from foods. J. Appl. Bacteriol., 65, 7–21. Go to original source... Go to PubMed...
    24. Walton A., Smith V.J. (1999): Primary culture of the haemocytes from marine decapods. Fish Shellfish Immunol., 99, 181–194. Go to original source...
    25. Weyant R.S., Moss C.W., Weaver R.E., Hollis D.G. Jordan J.G., Cook E.C., Daneshvar M.I. (1996): Identification of unusual pathogenic gram-negative bacteria. Second edition. Williams and Wilkins Baltimore, 15–16.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content