Vet Med - Czech, 2003, 48(6):147-154 | DOI: 10.17221/5762-VETMED

Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection

M. Toman, M. Faldyna, I. Pavlik
Veterinary Research Institute, Brno, Czech Republic

Several years lasting clinical and microbiological monitoring of a cattle herd infected with paratuberculosis allowed to identify three groups of animals showing different courses of the infection. Group N (non-shedders) included animals negative by faecal culture throughout the monitoring period. Animals of Group L (low shedders) shed sporadically small quantities of mycobacteria (1 to 9 CFU), but remained clinically healthy throughout the monitoring period. Group H (high shedders) included animals shedding repeatedly large quantities of Mycobacterium avium subsp. paratuberculosis (10 CFU) with a progressive deterioration of the state of health in most of them. Animals with specific antibodies detected by agar gel immunodiffusion and complement fixation test were found in all groups, but the percentage of serologically positive animals was higher in animals of Group L and significantly higher (p < 0.01) in animals of Group H than in animals of Group N. Specific cell-mediated immunity was demonstrated especially in the group of low shedders (Group L), the differences in the percentages of interferon gamma assay positive animals in this group and other groups was highly significant (p < 0.01). Only insignificant differences in the counts and activity of peripheral leukocytes were found among groups of clinically healthy animals differing in intensity of M. paratuberculosis shedding. However, the progressive development of clinical signs of paratuberculosis in Group H was associated with a significant (p < 0.01) decrease of lymphocytes and monocytes, a non-significant decrease of neutrophils, a significant (p < 0.05) increase in the percentage of CD4+ cells and a insignificant decrease in the percentage of CD8+ cells; consequently the CD4/CD8 ratio increased.

Keywords: Johne's disease; cattle; resistance; cell-mediated immunity; interferon-gamma

Published: June 30, 2003  Show citation

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Toman M, Faldyna M, Pavlik I. Immunological characteristics of cattle with Mycobacterium avium subsp. paratuberculosis infection. Vet Med - Czech. 2003;48(6):147-154. doi: 10.17221/5762-VETMED.
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    References

    1. Bassey E.O., Collins M.T. (1997): Study of T-lymphocyte subsets of healthy and Mycobacterium avium subsp. paratuberculosis-infected ca le. Infect. Immun., 65, 4869-4872. Go to original source... Go to PubMed...
    2. Bendixen P.H., Bloch B., Jorgensen J.B. (1981): Lack of intracellular degradation of Mycobacterium paratuberculosis by bovine macrophages infected in vitro and in vivo: light microscopic and electron microscopic observations. Am. J. Vet. Res., 42, 109-113. Go to original source...
    3. Billman-Jacobe H., Carrigan M., Cockram F., Corner L.A., Gill I.J., Hill J.F., Jessep T., Milner A.R., Wood P.R. (1992): A comparison of the interferon gamma assay with absorbed ELISA for diagnosis of Johne's disease in ca le. Aust. Vet. J., 69, 25-28. Go to original source... Go to PubMed...
    4. Buergelt C.D., Hall C.H.E., Merkal R.S., Whitlock R.H., Duncan J.R. (1977). Lymphocyte transformation: an aid in the diagnosis of paratuberculosis. Am. J. Vet. Res., 38, 1709-1715. Go to original source...
    5. Chiodini R.J. (1996): Immunology: resistance to paratuberculosis. Vet. Clin. North Am. Food Anim. Pract., 12, 313-343. Go to original source... Go to PubMed...
    6. Chiodini R.J., Davis W.C. (1992): The cellular immunology of bovine paratuberculosis: the predominant response is mediated by cytotoxic gamma/delta T lymphocytes which prevent CD4+ activity. Microb. Pathogen., 13, 447-463. Go to original source... Go to PubMed...
    7. Chiodini R.J., Van Kruiningen H.J., Merkal R.S. (1984): Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet., 72, 217-262.
    8. Collins M.T. (1996): Diagnosis of paratuberculosis. Vet. Clin. North Am. Food Anim. Pract., 12, 357-371. Go to original source... Go to PubMed...
    9. Coussens P.M. (2001): Mycobacterium paratuberculosis and the bovine system. Anim. Health Res. Rev., 2, 141-161. Go to original source...
    10. Horin P. (1998): Biological principles of heredity of a resistance to disease. Rev. Sci. Tech. Off. Int. Epiz., 17, 302-314. Go to original source... Go to PubMed...
    11. Horin P., Pavlik I., Koskova S. (1995): BoLa class-I haplotypes and Mycobacterium paratuberculosis in ca le. In: Proceedings from Fourth Veterinary Immunology Symposium, Davis, 114.
    12. Jungersen G., Huda A., Hansen J.J., Lind P. (2002): Interpretation of gamma interferon test for diagnosis of subclinical paratuberculosis in ca le. Clin. Diagn. Lab. Immunol., 9, 453-460. Go to original source... Go to PubMed...
    13. Koets A.P., Ru en V.P.M.G., Hoek A., Bakker D., Van Zijderveld F., Müller K.E., Van Eden V.V. (1999): Heatshock protein-specific T-cell responses in various stages of bovine paratuberculosis. Vet. Immunol. Immunopathol., 70, 105-115. Go to original source... Go to PubMed...
    14. Koets A.P., Adugna G., Janss L.L.G., Van Weering H.J., Kalis C.H.J., Wenting G.H., Ru en V.P.M.G., Schukken Y.H. (2000): Genetic variation of susceptibility to Mycobacterium avium subsp. paratuberculosis infection in dairy ca le. J. Dairy Sci., 83, 2702-2708. Go to original source... Go to PubMed...
    15. Koets A.P., Ru en V.P., de Boer M., Baker D., ValentinWeigand P., Van Eden W. (2001): Differential changes in shock protein-, lipoarabinomannan-, and purified protein derivative-specific imunoglobulin G1 and G2 isotype responses during bovine Mycobacterium avium subsp. paratuberculosis infection. Infect. Immun., 69, 1492-1498. Go to original source... Go to PubMed...
    16. Koets A.P., Ru en V.P., Hoek A., Van Mil F., Muller K., Bakker D., Gruys E., Van Eden W. (2002): Progressive bovine paratuberculosis is associated with local loss of CD4+ T cells, increased frequency of gamma delta T cells, and related changes in T cell function. Infect. Immun., 70, 3856-3864. Go to original source... Go to PubMed...
    17. Kunze Z.M., Portales S.F., McFadden J.J. (1992): Biologically distinct subtypes of Mycobacterium avium differ in possession in insertion sequence IS901. J. Clin. Microbiol., 30, 2366-2372. Go to original source... Go to PubMed...
    18. Matouskova O., Chalupa J., Cigler M., Hruska K. (1992): Stat Plus - Manual (in Czech). 1st ed. Veterinary Research Institute, Brno. 168 pp.
    19. Merkal R.S., McCullough W.G. (1982): A new mycobactin, mycobactin J, from Mycobacterium paratuberculosis. Curr. Microbiol., 7, 333-335. Go to original source...
    20. Merkal R.S., Kopecky K.E., Larsen A.B., Ness R.D. (1970): Immunologic mechanisms in bovine paratuberculosis. Am. J. Vet. Res., 31, 475-485.
    21. Pavlik I., Matlova L., Vesely T., Bartl J., Valent L., Miskovic P., Hirko M. (1999): Control of paratuberculosis in five ca le farms by serological test and faecal culture during the period 1990-1999. Vet. Med. - Czech., 45, 61-70.
    22. Pavlik I., Matlova L., Bartl J., Svastova P., Dvorska L., Whitllock R. (2000): Parallel faecal and organ Mycobacterium avium subsp. paratuberculosis culture of different productivity types of ca le. Vet. Microbiol., 77, 309- 324. Go to original source... Go to PubMed...
    23. Stabel J.R. (1996): Production of gama-interferon by peripheral blood mononuclear cells: an important diagnostic tool for detection of subclinical paratuberculosis. J. Vet. Diagn. Invest., 8, 345-350. Go to original source... Go to PubMed...
    24. Storset A.K., Berntsen G., Larsen H.J. (2000): Kinetics of IL-2 receptor expression on lymphocyte subsets from goats infected with Mycobacterium avium subsp. paratuberculosis a er specific in vitro stimulation. Vet. Immunol. Immunopathol., 77, 43-54. Go to original source... Go to PubMed...
    25. Sweeney R.W, Jones D.E., Habecker P., Sco P. (1998): Interferon-gama and interleukin 4 gene expression in cows infected with Mycobacterium paratuberculosis. Am. J. Vet. Res., 59, 842-847. Go to original source...
    26. Toman M., Svoboda M., Rybnicek J., Krejci J., Svobodova V. (1998): Secondary immunodefficiency in dogs with enteric, dermatologic, infectious or parasitic diseases. J. Vet. Med. (B), 45, 321-334. Go to original source... Go to PubMed...
    27. Veazey R.S., Horohov D.W., Krahenbuhl J.L., Taylor H.W., Oliver J.L., Snider T.G. (1995): Comparison of the resistance of C57Bl/6 and CRH/He mice to infection with Mycobacterium paratuberculosis. Vet. Microbiol., 47, 79-88. Go to original source... Go to PubMed...
    28. Whipple D.L., Callihan D.R., Jarnagin J.L. (1991): Cultivation of Mycobacterium paratuberculosis from bovine fecal specimens and a suggested standardised procedure. J. Vet. Diagn. Invest., 3, 368-373. Go to original source... Go to PubMed...
    29. Whitlock R.H., Buergelt C. (1996): Preclinical and clinical manifestations of paratuberculosis (including pathology). Vet. Clin. North Am. Food Anim. Pract., 12, 345-356. Go to original source... Go to PubMed...
    30. Wood P.R., Corner L.A., Rothel J.S., Ripper J.L., Fifis T., McCormick B.S., Francis B., Melville L., Small K., deWi e K., Tolson J., Ryan T.J., de Lisle G.W., Cox J.C., Jones S.J. (1992): A field evaluation of serological and cellular diagnostic tests for bovine tuberculosis. Vet. Microbiol., 31, 71-79. Go to original source... Go to PubMed...
    31. Yayo Ayele W., Machackova M., Pavlik I. (2001): The transmission and impact of paratuberculosis infection in domestic and wild ruminants. Vet. Med. - Czech, 46, 205-224. Go to original source...

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