Vet Med - Czech, 2004, 49(3):65-69 | DOI: 10.17221/5678-VETMED

Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats

G. Ozbey, A. Kilic, H. B Ertas, A. Muz
Department of Microbiology, Faculty of Veterinary Science, University of Firat, Elazig, Turkey

n this study, 30, 15 and 1 strains of Pasteurella multocida and 9, 8 and 6 strains of Mannheimia haemolytica from cattle, sheep and goats isolated in Elazig province located in the East of Turkey, respectively were typed by random amplified polymorphic DNA (RAPD) assay using a random primer (OPA-11). By RAPD assay, two and three distinct band profiles were obtained in the examination of P. multocida isolates from cattle and sheep, respectively. However, M. haemolytica isolates from cattle, sheep and goats showed only one profile and these strains were not discriminated by RAPD. This study showed that little genetic heterogeneity exists among P. multocida and M. haemolytica isolates from lungs of cattle, sheep and goats.

Keywords: Pasteurella multocida; Mannheimia haemolytica; random amplified polymorphic DNA; cattle; sheep and goats; typing

Published: March 31, 2004  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ozbey G, Kilic A, Ertas HB, Muz A. Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats. Vet Med - Czech. 2004;49(3):65-69. doi: 10.17221/5678-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Charlton B.R., Bickford A.A., Walker R.L., Yamamoto R. (1999): Complementary randomly amplified polymorphic DNA (RAPD) analysis pa erns and primer sets to differentiate Mycoplasma gallisepticum strains. J. Vet. Diagn. Invest., 11, 158-161. Go to original source... Go to PubMed...
    2. Chaslus-Dancla E., Lesage-Descauses M.C., Leroy-Setrin S., Martel J.L., Coudert P., Lafont J.P. (1996): Validation of random amplified polymorphic DNA assays by ribotyping as tools for epidemiological surveys of Pasteurella from animals. Vet. Microbiol., 52, 91-102. Go to original source... Go to PubMed...
    3. Chatellier S., Ramanantsoa C., Harriau P., Rolland K., Rosenau A., Quentin R., (1997): Characterization of Streptococcus agalactiae strains by randomly amplified polymorphic DNA analysis. J. Clin. Microbiol., 35, 2573-2579. Go to original source... Go to PubMed...
    4. Christiansen K.H., Carpenter T.E., Snipes K.P., Hird D.W., Ghazikhanian G.Y. (1992): Restriction endonuclease analysis of Pasteurella multocida isolates from three California turkey premises. Avian Dis., 36, 272-281. Go to original source... Go to PubMed...
    5. Dziva F., Christensen H., Olsen J.E., Mohan K. (2001): Random amplification of polymorphic DNA and phenotypic typing of Zimbabwean isolates of Pasteurella multocida. Vet. Microbiol., 82, 361-372. Go to original source... Go to PubMed...
    6. Fegan N., Blackall P.J., Pahoff J.L. (1995): Phenotypic characterisation of Pasteurella multocida isolates from Australian poultry. Vet. Microbiol., 47, 281-286. Go to original source... Go to PubMed...
    7. Gilmour N.J.L., Angus K.W., Gilmour J.S. (1991): Disease of Sheep. Martin W.B., Aitken I.D. (eds.), Blackwell Scientific Publications. 133-139.
    8. Hilton A.C., Mortiboy D., Banks J.G., Penn C.W. (1997): RAPD analysis of environmental, food and clinical isolates of Campylobacter spp. FEMS Immunol. Med. Microbiol., 18, 119-124. Go to original source... Go to PubMed...
    9. Huber B.S., Allred D.V., Carmen J.C., Frame D.D., Whiting D.G., Cryan J.R., Olson T.R., Jackson P.J., Hill K., Laker M.T., Robison R.A. (2002): Random amplified polymorphic DNA and amplified fragment length polymorphism analyses of Pasteurella multocida isolates from fatal fowl cholera infections. J. Clin. Microbiol., 40, 2163-2168. Go to original source... Go to PubMed...
    10. Jaworski M.D., Ward A.C., Hunter D.L., Wesley I.V. (1993): Use of DNA analysis of Pasteurella haemolytica biotype T isolates to monitor transmission in bighorn sheep (Ovis canadensis canadensis). J. Clin. Microbiol., 31, 831-835. Go to original source... Go to PubMed...
    11. Kim C.J., Nagaraja K.V. (1990): DNA fingerprinting for differentiation of field isolates from reference vaccine strains of Pasteurella multocida in turkeys. Am. J. Vet. Res., 51, 207-210. Go to original source...
    12. Kodjo A., Villard L., Bizet C., Martel J.L., Sanchis R., Borges E., Gauthier D., Maurin F., Richard Y. (1999): Pulsed-field gel electrophoresis is more efficient than ribotyping and random amplified polymorphic DNA analysis in discrimination of Pasteurella haemolytica strains. J. Clin. Microbiol., 37, 380-385. Go to original source... Go to PubMed...
    13. Lainson F.A., Aitchison K.D., Donachie W., Thomson J.R. (2002): Typing of Pasteurella multocida isolated from pigs with and without porcine dermatitis and nephropathy syndrome. J. Clin. Microbiol., 40, 588-593. Go to original source... Go to PubMed...
    14. Lam K.M., Yamamoto R., DaMassa A.J. (1995): DNA diversity among isolates of Campylobacter jejuni detected by PCR-based RAPD fingerprinting. Vet. Microbiol., 45, 269-274. Go to original source... Go to PubMed...
    15. Lee M.D., Mize M.S. (1997): RAPD analysis of serotype 3, 4 isolates of Pasteurella multocida. Poultry Sci., 76 (Suppl. 1) 147.
    16. Lin A.W., Usera M.A., Barre T.J., Goldsby R.A. (1996): Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis. J. Clin. Microbiol., 34, 870-876. Go to original source... Go to PubMed...
    17. Links I.J., Searson J.E., Godwin J., Glastonbury J.R., Philbey A.P., Ma hews L.M. (1992): Pasteurella multocida and Pasteurella haemolytica infections in ruminants and pigs in Southern New South Wales. In: Pa en B.E., Spencer T.L., Johnson R.B., Hoffman D., Lehane L. (eds.): Pasteurellosis in Production Animals. ACIAR Proceedings No. 43, 108-111.
    18. Loubinoux J., Lozniewski A., Lion C., Garin D., Weber M., Le Faou A.E. (1999): Value of enterobacterial repetitive intergenic consensus PCR for study of Pasteurella multocida strains isolated from mouths of dogs. J. Clin. Microbiol., 37, 2488-2492. Go to original source... Go to PubMed...
    19. Magarinos B., Romalde J.L., Bandin I., Fouz B., Toranzo A.E. (1992): Phenotypic, antigenic, and molecular characterization of Pasteurella piscicida strains isolated from fish. Appl. Environ. Microbiol., 58, 3316-3322. Go to original source... Go to PubMed...
    20. Maurer J.J., Lee M.D., Lobsinger C., Brown T., Maier M., Thayer S.G. (1998): Molecular typing of avian Escherichia coli isolates by random amplification of polymorphic DNA. Avian Dis., 42, 431-451. Go to original source... Go to PubMed...
    21. Mohan K., Sadza M., Madsen M., Hill F.W., Pawandiwa A. (1994): Phenotypic characterization of Zimbabwean isolates of Pasteurella multocida. Vet. Microbiol., 38, 351-357. Go to original source... Go to PubMed...
    22. Mohan K., Kelly P.J., Hill F.W.G., Muvavarirwa P., Pawandiwa A. (1997): Phenotype and serotype of Pasteurella multocida isolates from diseases of dogs and cats in Zimbabwe. Comp. Immunol. Microbiol. Infect Dis., 20, 29-34. Go to original source... Go to PubMed...
    23. Murphy G.L., Robinson L.C., Burrows G.E. (1993): Restriction endonuclease analysis and ribotyping differentiate Pasteurella haemolytica serotype A1 isolates from ca le within a feedlot. J. Clin. Microbiol., 31, 2303-2308. Go to original source... Go to PubMed...
    24. Neumann S., Leeb T., Brenig B. (1998): Vergleich zwischen PCR und kultureller untersuchung zum nachweis von infektionen mit Pasteurella multocida beim hund. Kleintierpraxis, 43, 69-74.
    25. Quinn P.J., Carter M.E., Markey B., Carter G.R. (1994): Pasteurella species. Clin. Vet. Microbiol. Wolfe Pupl., Dublin, 254-259.
    26. Rhoades K.R., Rimler R.B. (1987): Capsular groups of Pasteurella multocida isolated from avian hosts. Avian Dis., 31, 895-898. Go to original source... Go to PubMed...
    27. Rhoades K.R., Rimler R.B. (1990): Somatic serotypes of Pasteurella multocida strains isolated from avian hosts (1976-1988). Avian Dis., 34, 193-195. Go to original source... Go to PubMed...
    28. Rimler R.B., Brogden K.A. (1986): Pasteurella multocida isolated from rabbits and swine: serologic types and toxin production. Am. J. Vet. Res., 47, 730-737. Go to original source...
    29. Snipes K.P., Hirsh D.C., Kasten R.W., Hansen L.M., Hird D.W., Carpenter T.E., McCapes R.H. (1989): Use of an rRNA probe and restriction endonuclease analysis to fingerprint Pasteurella multocida isolated from turkeys and wildlife. J. Clin. Microbiol., 27, 1847-1853. Go to original source... Go to PubMed...
    30. Snipes K.P., Kasten R.W., Wild M.A., Miller M.W., Jessup D.A., Silflow R.L., Foreyt W.J., Carpenter T.E. (1992): Using ribosomal RNA gene restriction pa erns in distinguishing isolates of Pasteurella haemolytica from bighorn sheep (Ovis canadensis). J. Wildl. Dis., 28, 347-354. Go to original source... Go to PubMed...
    31. Tambic A., Power E.G., Talsania H., Anthony R.M., French G.L. (1997): Analysis of an outbreak of non-phage-typeable methicillin-resistant Staphylococcus aureus by using a randomly amplified polymorphic DNA assay. J. Clin. Microbiol., 35, 3092-3097. Go to original source... Go to PubMed...
    32. Weigler B.J., Thigpen J.E., Goelz M.F., Babineau C.A., Forsythe D.B. (1996): Randomly amplified polymorphic DNA polymerase chain reaction assay for molecular epidemiologic investigation of Pasteurella pneumotropica in laboratory rodent colonies. Lab. Anim. Sci., 46, 386-392.
    33. Welsh J., McClelland M. (1990): Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res., 18, 7213-7218. Go to original source... Go to PubMed...
    34. Williams J.G., Kubelik A.R., Livak K.J., Rafalski J.A., Tingey S.V. (1990): DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18, 6531-6535. Go to original source... Go to PubMed...
    35. Wilson M.A., Morgan M.J., Barger G.E. (1993): Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates. J. Clin. Microbiol., 31, 255-259. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content