Vet Med - Czech, 2007, 52(11):496-501 | DOI: 10.17221/2063-VETMED

Prevalence of Campylobacter subtypes in pheasants (Phasianus colchicus spp. torquatus) in the Czech Republic

M. Nebola, G. Borilova, I. Steinhauserova
Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic

At the turn of 2005 and 2006, cecal samples from 302 pheasants, from two flocks from a farm with intensive production, and from 200 wild birds were collected. The prevalence of Campylobacter spp. in the intestinal contents of pheasants from the farm was 70.20%. 50.47% of isolated strains were identified as C. coli and 41.40% as C. jejuni. In samples from wild pheasants, positive cultivation of Campylobacter spp. was proven in 27.5% of cases. A total of 15 PFGE subtypes were noted among the Campylobacter spp. isolates. C. jejuni strains could be divided into 16 fla-RFLP subtypes. No PFGE profile specific for C. jejuni and C. coli, respectively, was found. When comparing wild birds with farmed pheasants, there were eight possible combinations of PCR/RFLP and PFGE subtypes in wild birds and 40 combinations in farmed pheasants. No preferred combination of both subtypes was detected and all combinations were randomly ordered. To determine the significance of pheasants as a source of C. jejuni infection in humans, the RFLP and PFGE patterns of pheasant isolates are currently being compared with those of isolates from diarrheic patients.

Keywords: Campylobacter jejuni; Campylobacter coli; PCR, RFLP; PFGE

Published: November 30, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Nebola M, Borilova G, Steinhauserova I. Prevalence of Campylobacter subtypes in pheasants (Phasianus colchicus spp. torquatus) in the Czech Republic. Vet Med - Czech. 2007;52(11):496-501. doi: 10.17221/2063-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Atanassova V., Ring C. (1999): Prevalence of Campylobacter spp. in poultry and poultry meat in Germany. International Journal of Food Microbiology, 51, 187-190. Go to original source... Go to PubMed...
    2. Broman T., Palmgren H., Bergstromet S., Sellin M., Waldenstrom J., Danielsson-Tham M.-L., Olsen B. (2002): Campylobacter jejuni in Black-Headed gulls (Larus ridibundus): prevalence, genotypes, and influence on C. jejuni epidemiology. Journal of Clinical Microbiology, 40, 4594-4602. Go to original source... Go to PubMed...
    3. Broman T., Waldenstrom J., Dahlgren D., Carlsson I., Eliasson I., Olsen B. (2004): Diversities and similarities in PFGE profiles of Campylobacter jejuni isolated from migrating birds and humans. Journal of Applied Microbiology, 96, 834-843. Go to original source... Go to PubMed...
    4. Chuma T., Hashimoto S., Okamoto K. (2000): Detection of thermophilic campylobacter from sparrows by multiplex PCR: the role of sparrows as a source of contamination of broilers with Campylobacter. Journal of Veterinary Medical Science, 62, 1291-1295. Go to original source... Go to PubMed...
    5. Devane M.L., Nicol C., Ball A., Klena J.D., Scholes P., Hudson J.A., Baker M.G., Gilpin B.J., Garrett N., Savill M.G. (2005): The occurence of Campylobacter subtypes in environmental reservoirs and potential transmission routes. Journal of Applied Microbiology, 98, 980-990. Go to original source... Go to PubMed...
    6. Engvall E.O., Brandstram B., Gunnarsson A., Morner T., Wahlstrom H., Fermer C. (2002): Validation of polymerase chain reaction/restriction enzyme analysis method for species identification of thermophilic campylobacters isolated from domestic and wild animals. Journal of Applied Microbiology, 92, 47-54. Go to original source... Go to PubMed...
    7. Fermer C.H., Engvall E.O (1999): Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. Journal of Clinical Microbiology, 37, 3370-3373. Go to original source... Go to PubMed...
    8. Fitzgerald C., Stanley K., Andrew S., Jones K. (2001): Use of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of Campylobacter jejuni and Campylobacter coli in farm and clinical environments. Applied and Environmental Microbiology, 67, 1429-1436. Go to original source... Go to PubMed...
    9. Guevremont E., Higgins R., Quessy S. (2004): Characterization of Campylobacter isolates recovered from clinically healthy pigs and from sporadic cases of campylobacteriosis in humans. Journal of Food Protection, 67, 228-234. Go to original source... Go to PubMed...
    10. Hanninen M.-L., Hakkinen M., Rautelin H. (1999): Stability of related human and chicken Campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis. Applied and Environmental Microbiology, 65, 2272-2275. Go to original source... Go to PubMed...
    11. Heryford A.G., Seys S.A. (2004): Outbreak of occupational campylobacteriosis associated with a pheasant farm. Journal of Agricultural Safety and Health, 10, 127-132. Go to original source... Go to PubMed...
    12. Korolik V., Alderton M.R., Smith S.C., Chang J., Coloe P.J. (1998): Isolation and molecular analysis of colonising and noncolonising strains of Campylobacter jejuni and Campylobacter coli following experimental infection of young chickens. Veterinary Microbiology, 60, 239-249. Go to original source... Go to PubMed...
    13. Levesque B., Brousseau P., Bernier F., Dewailly E., Joly J. (2000): Study of the bacterial content of ring-billed gull droppings in relation to recreational water quality. Water Research, 34, 1089-1096. Go to original source...
    14. Manning G., Duim B., Wassenaar T., Wagenaar J.A., Ridley A., Newell D.G. (2001): Evidence for a genetically stable strain of Campylobacter jejuni. Applied and Environmental Microbiology, 67, 1185-1189. Go to original source... Go to PubMed...
    15. Nishimura M., Nukina M., Yuan J.M., Shen B.Q., Ma J. J., Otha M., Saida T. Uchiyama T. (1996): PCR-based restriction fragment length polymorpism (RFLP) analysis and serotyping of Campylobacter jejuni isolates from diarrheic patients in China and Japan. FEMS Microbiology Letters, 142, 133-138. Go to original source... Go to PubMed...
    16. Padungton P., Kaneene J.B. (2003): Campylobacter spp. in human, chickens, pigs and their antimicrobial resistance. Journal of Veterinary Medicine and Sciences, 65, 161-170. Go to original source... Go to PubMed...
    17. Petersen L., Nielsen E.M., On S.L.W. (2001): Serotype and genotype diversity and hatchery transmission of Campylobacter jejuni in commercial poultry flocks. Veterinary Microbiology, 82, 141-154. Go to original source... Go to PubMed...
    18. Steinhauserova I., Ceskova J., Nebola M. (2002): PCR/ restriction length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains. Letters in Applied Microbiology, 34, 354-358. Go to original source... Go to PubMed...
    19. Stern J.N., Bannov A.V., Svetoch A.E., Mitschevich V.E., Mitschevich P.I., Volozhantsev V. N., Gusev V.V., Perelygin V.V. (2004): Distribution and characterization of Campylobacter spp. from Russian poultry. Journal of Food Protection, 67, 239-245. Go to original source... Go to PubMed...
    20. Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persing D.H. (1995): Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis criteria for bacterial strain typing. Journal of Clinical Microbiology, 33, 2233-2239. Go to original source... Go to PubMed...
    21. Waldenstrom J., Broman T., Carlsson I., Hasselquist D., Achterberg R.P., Wagenaar J.A., Olsen B. (2002): Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. Applied and Environmental Microbiology, 68, 5911-5917. Go to original source... Go to PubMed...
    22. Wassenaar T.M., Fry B.N., van der Zeijst B.A. (1995): Variation of the flagellin gene locus of Campylobacter jejuni by recombination and horizontal gene transfer. Microbiology, 141, 95-101. Go to original source... Go to PubMed...
    23. Workman S.N., Mathison G.E., Lavoie M.C. (2005): Pet dogs and chicken meat as reservoirs of Campylobacter spp. in Barbados. Journal of Clinical Microbiology. 43, 2642-2650. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content