Vet Med - Czech, 2012, 57(11):583-590 | DOI: 10.17221/6465-VETMED

Dynamics of milk leukocytes in response to intramammary infusion of amoxicillin plus sulbactam during bovine subclinical mastitis

U.K. De, R. Mukherjee
Veterinary Research Institute, Izatnagar, Uttar Pradesh, India

The therapeutic potential of amoxicillin plus sulbactam and its effect on dynamics of milk leukocytes in bovine sub clinical mastitis were investigated in this study. Therapeutic efficacy was measured by somatic cell count and total bacterial count of the milk, whereas, the dynamics of milk leukocytes were assessed by measuring phagocytosis, hydrogen peroxide production, myeloperoxidase and lactoperoxidase enzyme levels in the milk leukocytes. Forty-five crossbred cows were randomly divided into three equal groups. Group I consisting of 15 cows served as healthy control, whereas 30 cows (sub clinical mastitis) were randomly divided into Groups II and III on the basis of positive reactions in the California Mastitis Test. Group II cows received 300mg of amoxicillin plus sulbactam twice daily for three days and Group III received sterile 5 ml phosphate buffer saline (pH 7.4) for three days. Both treatments were administered via the intramammary route. Observations were made up to 15 days after initiation of treatment. The results revealed a pronounced drop in somatic cell count and total bacterial count, whereas significant (P < 0.05) enhancement of phagocytic activity (42.20%), hydrogen peroxide production (29.46%), myeloperoxidase (49.27%) and lactoperoxidase (147.10%) enzyme levels in the milk leukocytes in Group II cows during post treatment periods were observed. Such changes were statistically non-significant in Group III cows. The results of the present study indicate that intramammary use of amoxicillin plus sulbactam augments the bactericidal function of milk leukocytes during bovine sub clinical mastitis and demonstrate the strong therapeutic potential against bovine subclinical mastitis.

Keywords: amoxicillin; leukocytes; subclinical mastitis; sulbactam

Published: November 30, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
De UK, Mukherjee R. Dynamics of milk leukocytes in response to intramammary infusion of amoxicillin plus sulbactam during bovine subclinical mastitis. Vet Med - Czech. 2012;57(11):583-590. doi: 10.17221/6465-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Anderson R, Joone G, van Rensburg CEJ (1986): An in vitro investigation of the intracellular bioactivity of Amoxicillin, clindamycin, and erythromycin for Staphylococcus aureus. Journal of Infectious Disease 153, 593-600. Go to original source... Go to PubMed...
    2. Boyne R, Arthur JR (1979): Alteration of neutrophil function in selenium-deficient cattle. Journal of Comparative Pathology 89, 151-158. Go to original source... Go to PubMed...
    3. Bretz U, Baggiolini M (1974): Biochemical and morphological characterization of azurophilic and specific granules of human neutrophilic polymorphonuclear leucocytes. Journal Cellular Biology 63, 251-269. Go to original source... Go to PubMed...
    4. Burvenich C, Paape MJ, Hill AW, Guidry AJ, Miller RH, Heyeneman R, Kremer WDJ (1994): Role of the neutrophil leukocyte in the local and systemic reaction during experimentally induced E. coli mastitis in cows immediately after calving. Veterinary Quarterly 16, 45-50. Go to original source... Go to PubMed...
    5. Cai TQ, Weston PG, Lung LA, Brodie B, Mekenna DJ, Wagner WC (1994): Association between neutrophil functions and periparturient disorders in cows. American Journal of Veterinary Research 55, 934-943. Go to original source...
    6. Cattell MB, Dinsmore RP, Belschner AP, Carmen J, Goodell G (2001): Environmental gram-positive mastitis treatment: In vitro sensitivity and bacteriologic cure. Journal of Dairy Science 84, 2036-2043. Go to original source... Go to PubMed...
    7. Cooray RA, Bjorek L (1995): Bactericidal activity of the bovine myeloperoxidase system against bacteria associated with mastitis. Veterinary Microbiology 46, 427-434. Go to original source... Go to PubMed...
    8. Cuffini AM, De Renzi G, Tullio V, Paizis G, Carlone NA (1996): Potentiation of human polymorphonuclear leukocyte phagocytosis and intracellular bactericidal activity by amoxycillin/clavulanate. Drugs Under Experimental and Clinical Research 22, 9-15. Go to PubMed...
    9. Cuffini AM, Tullio V, Ianni Palarchio A, Bonino A, Paizis G, Carlone NA (1998): Enhanced effects of amoxycillin/clavulanate compared with amoxycillin and clavulanate alone on the susceptibility to immunodefenses of a penicillin-resistant strain of Streptococcus pneumoniae. Drugs Under Experimental and Clinical Research 24, 173-184. Go to PubMed...
    10. Cuffini AM, Tullio V, Giacchino F, Bonino A, Mandras N, Bianchi N, Roana J, Scalas D, Bonello F, Carlone NA (2001a): Improved phagocyte response by coamoxiclav in renal transplant recipients. Transplantation 71, 575-577. Go to original source... Go to PubMed...
    11. Cuffini AM, Tullio V, Giacchino F, Mandras N, Scalas D, Belardi P, Merlino C, Carlone NA (2001b): Impact of co-amoxiclav on polymorphonuclear granulocytes from chronic hemodialysis patients. American Journal of Kidney Disease 37, 1253-1259. Go to original source... Go to PubMed...
    12. Daley MJ, Oldham ER, Williams TJ, Coyle PA (1991): Quantitative and qualitative properties of host PMN cells during experimentally induced Staphylococcus aureus mastitis in cows. American Journal of Veterinary Research 52, 74-79. Go to original source...
    13. De UK, Mukherjee R (2009): Expression of cytokines and respiratory burst activity of milk cells in response to Azadirachta indica during bovine mastitis. Tropical Animal Health and Production 41, 189-197. Go to original source... Go to PubMed...
    14. De-Oliveira AP, Watts JL, Salmon SA, Aarestrup FM (2000): Antimicrobial susceptibility of Staphylococcus aureus isolated from bovine mastitis cases in Europe and United States. Journal of Dairy Science 83, 855-862. Go to original source... Go to PubMed...
    15. Dias IH, Matthews JB, Chapple IL, Wright HJ, Dunston CR, Griffiths HR (2011): Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines. Journal of Clinical Periodontology 38, 1-7. Go to original source... Go to PubMed...
    16. Dosogne H, Hoeben D, Burvenich C, Lohuis JACM (1999): Effect of Cephapirin and Mecillinam on the phagocytic and respiratory burst activity of neutrophil leucocytes isolatedfrom bovine blood. Journal of Veterinary Pharmacology and Therapeutics 21, 421-427 Go to original source... Go to PubMed...
    17. Finlay J, Miller L, Poupard JA (2003): A review of the antimicrobial activity of clavulanate. Journal of Antimicrobial and Chemotherapy 52, 18-23. Go to original source... Go to PubMed...
    18. Gomez-Lus ML, Aguilar L, Martin M, Gimenez MJ, Martínez P, Prieto J (1997): Intracellular and extracellular killing of a penicillin-resistant, serotype-9 strain of Streptococcus pneumoniae by polymorphonuclear leucocytes in the presence of sub-inhibitory concentrations of clavulanic acid. Journal of Antimicrobial and Chemotherapy 40, 142-144. Go to original source... Go to PubMed...
    19. Gruet P, Maincent P, Berthelot X, Kaltsatos V (2001): Bovine mastitis and intramammary drug delivery: review and perspectives. Advanced Drug Delivery Reviews 50, 245-259. Go to original source... Go to PubMed...
    20. Hellberg L, Fuchs S, Gericke C, Sarkar A, Behnen M, Solbach W, Laskay T (2011): Proinflammatory stimuli enhance phagocytosis of apoptotic cells by neutrophil granulocytes. Scientific World Journal 11, 2230-2236. Go to original source... Go to PubMed...
    21. Hoeben D, Burvenich C, Heneman R (1998): Antibiotics commonly used to treat mastitis and respiratory burst of bovine polymorphonuclear leucocytes. Journal of Dairy Science 81, 403-410. Go to original source... Go to PubMed...
    22. Hoeben D, Monfardini E, Opsomer G, Burvenich C, Dosogne H, Kruif AD, Beckers JF (2000): Chemiluminescence of bovine polymorphonuclear leukocytesduring the periparturient period and relation withmetabolic markers and bovine pregnancy associatedglycoprotein. Journal of Dairy Research 67, 249-259. Go to original source... Go to PubMed...
    23. Karmakar UK, Datta BK, Patra PH, Prashant M, Suman S, Sar TK, Chakraborty AK, Mandal TK (2011): Disposition kinetics of ceftizoxime in healthy and mastitic goats after intravenous administration. Pharmacologyonline 3, 935-946.
    24. Kazmierczak A, Pechinot A, Siebor E, Cordin X, Labia R (1989): Sulbactam: secondary mechanisms of action. Diagnostic Microbiology and Infectious Disease 12, 139S-146S. Go to original source... Go to PubMed...
    25. Kehrli ME, Shuster DE (1994): Factors affecting milk somatic cell count and their role in health of bovine mammary gland. Journal of Dairy Science 77, 619-627. Go to original source... Go to PubMed...
    26. Kolmer JA, Spaulding EH, Robinson HW (1951): Approved Laboratory Technic. Methods for the Bacteriologic Examination of Milk. 5th ed. Appleton-Century-Crofts Inc, New York, USA. 586-593.
    27. Korhonen H (1980): Potential role of the lactoperoxidase system (LP/SCN-/H2O2) in mastitis resistance. In: Bassalik-Chabielska L, Ryniewicz Z (eds.): Proceedings of International Conference on Resistant Factors and Genetic Aspects of Mastitis Control. Jablonna, Poland, 2-5 October, 421-438.
    28. Labro MT (2000): Interference of antibacterial agents with phagocyte functions: Immunomodulation or "ImmunoFairy Tales"? Clinical Microbiology Reviews 13, 615-650. Go to original source... Go to PubMed...
    29. Marshall VME, Cole WM, Bramley AJ (1986): Influence of lactoperoxidase system on susceptibility of the udder to Streptococcus uberis infection. Journal of Dairy Research 53, 507-514. Go to original source... Go to PubMed...
    30. Mehrzad J, Dosogne H, Meyer E, Burvenich C (2001): Local and systemic effects of endotoxin mastitis on the chemiluminescence of milk and blood neutrophils in dairy cows. Veterinary Research 32, 131-144. Go to original source... Go to PubMed...
    31. Melhus A, Ryan AF (2004): Effects of amoxicillin on cytokine and osteocalcin expression in bone tissue during experimental acute otitis media. Cytokine 25, 254-259. Go to original source... Go to PubMed...
    32. Mukherjee R, Ram GC, Dash PK, Ram GC (2005): Immunotharapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis. Research in Veterinary Science 79, 37-43. Go to original source... Go to PubMed...
    33. Pascual A, Martinez Martinez L, Aragon J, Perea EJ (1989): Effect of amoxycillin and clavulanic acid, alone and in combination, on human polymorphonuclear leukocyte function against Staphylococcus aureus. Europen Journal of Clinical Microbiology and Infectious Diseases 8, 277-281. Go to original source... Go to PubMed...
    34. Pick E, Keisari Y (1980): A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. Journal of Immunological Methods 38, 161-170. Go to original source... Go to PubMed...
    35. Reato G, Cuffini AM, Tullio V, Mandras N, Roana J, Banche G, Foa R, Carlone NA (2004): Immunomodulating effect of antimicrobial agents on cytokine production by human polymorphonuclear neutrophils. International Journal of Antimicrobial Agents 23, 150-154. Go to original source... Go to PubMed...
    36. Reato G, Cuffinia AM, Tullioa V, Ianni Palarchio A, Boninoa A, Foab R, Carlonea NA (1999): Co-amoxiclav affects cytokine production by human polymorphonuclear cells. Journal of Antimicrobial and Chemotherapy 43, 715-718. Go to original source... Go to PubMed...
    37. Santos JI, Arbo A (1989): The in vitro effect of sulbactam on polymorphonuclear leukocyte function. Diagnostic Microbiology and Infectious Disease 12, 147S-152S. Go to original source... Go to PubMed...
    38. Schalm OW, Carrol EJ, Jain NC (1971): Bovine Mastitis. Lea and Febiger, Philadelphia. 128-129.
    39. Seegers H, Fourichon C, Beaudeau F (2003): Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research 34, 475-491. Go to original source... Go to PubMed...
    40. Seifu E, Buys EM, Donkin EF, Petzer IM (2004): Antibacterial activity of the lactoperoxidase system against food-borne pathogens in Saanen and South African Indigenous goat milk. Food Control 15, 447-452. Go to original source...
    41. Sharma N, Mukherjee R, Ingale SL, Jadhav RK (2010): Therapeutic and anti-oxidant activity of vitamin E and selenium in bovine Staphylococcal mastitis. Indian Journal of Veterinary Research 19, 25-31.
    42. Shem MN, Malole JML, Machangu R, Kurwijila LR, Fujihara T (2001): Incidence and causes of sub-clinical mastitis in dairy cows on smallholder and large scale farms in tropical areas of Tanzania. Asian Australasian Journal of Animal Sciences 14, 372-377. Go to original source...
    43. Sisecioglu M, Uguz MT, Cankaya M, Ozdemir H, Gulcin I (2011): Effects of Ceftazidime Pentahydrate, Prednisolone, Amikacin Sulfate, Ceftriaxone Sodium and Teicoplanin on bovine milk lactoperoxidase activity. International Journal of Pharmacology 7, 79-83. Go to original source...
    44. Sordillo LM, Streicher1 KL (2002): Mammary Gland Immunity and Mastitis Susceptibility. Journal of Mammary Gland Biology and Neoplasia 7, 135-146. Go to original source... Go to PubMed...
    45. Sordillo LM, Weaver SH, DeRosa D (1997): Symposium immunology. Immunobiology of the mammary gland. Journal of Dairy Science 80, 1851-1865. Go to original source... Go to PubMed...
    46. Tenovuo J, Knuuttila MLE (1997): The antibacterial action of the various components of the lactoperoxidase system on a cariogenic strain of Streptococcus mutans. Journal of Dental Research 56, 1603-1607. Go to original source... Go to PubMed...
    47. Tufani NA, Hafiz A, Peer FU, Makhdoomi DM, Qureshi SD (2010): Clinico-therapeutic management of gangrenous mastitis in ovines. Indian Journal of Small Ruminants 16, 145-147.
    48. Van Den Broek PJ (1989): Antimicrobial drugs, microorganisms, and phagocytes. Reviews of Infectious Diseases 11, 213-245. Go to original source... Go to PubMed...
    49. Weber K, Peterhans E, Wyler R (1983): The chemiluminiscent response of bovine polymorphonuclear leukocytes isolated from milk and blood. Veterinary Immunology and Immunopathology 4, 397-412. Go to original source... Go to PubMed...
    50. Wilson DJ, Gonzalez RN, Case KL, Garrison LL, Grohn YT (1999): Comparison of seven antibiotic treatments with no treatment for bacteriological efficacy against bovine mastitis pathogens. Journal of Dairy Science 82, 1664-1670. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content