Vet Med - Czech, 2015, 60(10):533-538 | DOI: 10.17221/8491-VETMED

Serological and molecular detection of Anaplasma phagocytophilum in horses reared in KoreaOriginal Paper

S.H. Lee1, K.T. Kim2, S.H. Yun3, E. Choi4, G.H. Lee5, Y.S. Park6, K.H. Cho7, S. Yi8, O.D. Kwon1, T.H. Kim1, D. Kwak1
1 College of Veterinary Medicine, Kyungpook National University, Buk-gu, Daegu, Republic of Korea
2 Animal Health Center of Zoo Land, Daejeon O-World Theme Park, Daejeon, Republic of Korea
3 Incheon Grand Park Children Zoo, Namdong-gu, Incheon Metropolitan, Republic of Korea
4 Smile Equine Clinic, Gangseo-gu, Busan, Republic of Korea
5 Seowon Equine Clinic, Jeju-si, Jeju, Republic of Korea
6 Korea National College of Agriculture and Fisheries, Jeonju, Jeollabuk-do, Republic of Korea
7 Division of Livestock Management, Gyeongsangbuk-do, Daegu, Republic of Korea
8 Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea

The objective of this study was to investigate the nationwide prevalence of Anaplasma phagocytophilum in horses reared in Korea using a commercial ELISA kit and two different nested PCR (nPCR) analyses. Our analysis showed that 2.9% (true prevalence: 3.1%, 16/549) of the horses were seropositive by ELISA, and none were positive by the two nPCR analyses. Significant differences were observed in the ELISA results when the data were analysed according to breed and geographic region. In light of a recent case of human granulocytic anaplasmosis in Korea and the predicted increase in the number and distribution of ticks due to global warming, continuous monitoring and appropriate control programs for Anaplasma spp. and other tick-borne pathogens need to be established.

Keywords: anaplasmosis; ELISA; equine; PCR; Korea

Published: October 31, 2015  Show citation

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Lee SH, Kim KT, Yun SH, Choi E, Lee GH, Park YS, et al.. Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea. Vet Med - Czech. 2015;60(10):533-538. doi: 10.17221/8491-VETMED.
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