Vet Med - Czech, 2015, 60(12):691-695 | DOI: 10.17221/8583-VETMED

Assessment of oxidative stress in Flathead mullet (Mugil cephalus) and Gilthead sea bream (Sparus aurata)Original Paper

F. Fazio1, G. Piccione1, C. Saoca1, A.R. Caputo2, S. Cecchini3
1 Department of Veterinary Science, University of Messina, Messina, Italy
2 Council for Research and Experimentation in Agriculture, Research Unit for Livestock Extensive, Potenza. Italy
3 Department of Sciences, University of Basilicata, Potenza, Italy

In this work we compared two species of fish with different feeding habits: Flathead mullet (Mugil cephalus) and Gilthead sea bream (Sparus aurata). The aim of this study was to evaluate total oxidant status (TOS), total antioxidant capacity (TAC) and TOS/TAC ratio (OSI), in order to highlight the presence of any differences and correlations in these two different species of fish. Thirty adult fish of Mugil cephalus and thirty of Sparus aurata were used. From each fish 0.6 ml of blood was collected. TOS and TAC indicators were measured in serum obtained from samples previously clotted and centrifuged. Our results showed statistically significant differences between the two species in TAC. TOS and OSI did not show significant differences between Gilthead sea bream and Flathead mullet. A positive relationship between TOS and TAC was found in Flathead mullet (Mugil cephalus), and a negative relation between TOS and TAC in Gilthead sea bream (Sparus aurata). Our study indicates that the oxidative status and the relationship between total oxidant status (TOS) and total antioxidant capacity (TAC) in serum are probably dependent on the fish species and are affected by different feeding habits.

Keywords: total oxidant status; total antioxidant capacity; teleost species; redox balance

Published: December 31, 2015  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Fazio F, Piccione G, Saoca C, Caputo AR, Cecchini S. Assessment of oxidative stress in Flathead mullet (Mugil cephalus) and Gilthead sea bream (Sparus aurata). Vet Med - Czech. 2015;60(12):691-695. doi: 10.17221/8583-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alberti A, Bolognini L, Macciantelli D, Caratelli A (2000): The radical cation of N,N diethyl-para-phenylendiamine: a possible indicator of oxidative stress in biological samples. Research on Chemical Intermediates 26, 253-267. Go to original source...
    2. Bayir A, Necdet Sirkecioglu A, Bayir M, Ibrahim Haliloglu H, Mahmut Kocaman E, Mevlut Aras N (2011): Metabolic responses to prolonged starvation, food restriction and refeeding in the brown trout, Salmo trutta: Oxidative stress and antioxidant defenses. Comparative Biochemistry and Physiology Part B 159, 191-196. Go to original source... Go to PubMed...
    3. Benzie IFF, Strain JJ (1996): The ferric reducing ability of plasma (FRAP) as measure of "antioxidant power": the FRAP assay. Analytical Biochemistry 239, 70-76. Go to original source... Go to PubMed...
    4. Di Giulio RT, Meyer JN (2008): Reactive oxygen species and oxidative stress. In: Di Giulio RT, Hinton DE (eds.): The Toxicology of Fishes. CRC Press, Taylor and Francis Group, New York. 273-324. Go to original source...
    5. Fazio F, Cecchini S, Faggio C, Caputo AR, Piccione G (2014): Stability of oxidative stress biomarkers in flathead mullet, Mugil cephalus, serum during short-term storage. Ecological Indicators 46, 188-142. Go to original source...
    6. Furne M, Garcia-Gallego M, Hidalgo MC, Morales AE, Domezain A, Domezain J, Sanz A (2009): Oxidative stress parameters during starvation and refeeding periods in Adriatic sturgeon (Acipenser naccarii) and rainbow trout (Oncorhynchus mykiss). Aquaculture Nutrition 15, 587-595. Go to original source...
    7. Grim JM, Simonik EA, Semones MC, Kuhn DE, Crockett EL (2013): The glutathione dependent system of antioxidant defence is not modulated by temperature acclimation in muscle tissues from striped bass, Morone saxatilis. Comparative Biochemistry and Physiolology, Part A 164, 383-390. Go to original source... Go to PubMed...
    8. Livingstone DR (2003): Oxidative stress in aquatic organism in relation to pollution and agriculture. Revue de Medecine Veterinaire 154, 427-430.
    9. Madeira D, Narciso L, Cabral HN, Vinagre C, Diniz MS (2013): Influence of temperature in thermal and oxidative stress responses in estuarine fish. Comparative Biochemistry and Physiology, Part A 166, 237-243. Go to original source... Go to PubMed...
    10. Martinez-Alvarez RM, Morales AE, Sanz A (2005): Antioxidant defences in fish: biotic and abiotic factors. Reviews in Fish Biology and Fisheries 15, 75-88. Go to original source...
    11. Nijveldt RJ, van Nood E, van Hoorn DEC, Boelens PG, van Norren K, van Leeuwen PAM (2001): Flavonoids: a review of probable mechanisms of action and potential applications. American Journal of Clinical Nutrition 74, 418-425. Go to original source... Go to PubMed...
    12. Nishida Y (2011): The chemical process of oxidative stress by copper (II) and iron (III) ions in several neurodegenerative disorders. Monatshefte fur Chemie 142, 375-384. Go to original source...
    13. Paital B, Chainy GB (2010): Antioxidant defenses and oxidative stress parameters in tissues of mud crab (Scylla serrata) with reference to changing salinity. Comparative Biochemistry and Physiology, Part C 151, 142-151. Go to original source... Go to PubMed...
    14. Pita C, Gamito S, Erzini K (2002): Feeding habits of the gilthead seabream (Sparus aurata) from the Ria Formosa (southern Portugal) as compared to the black seabream (Spondyliosoma cantharus) and the annular seabream (Diplodus annularis). Journal of Applied Ichthyology 18, 81-86. Go to original source...
    15. Po E, Williams C, Muscatello G, Celi P (2013): Assessment of oxidative stress biomarkers in exhaled breath condensate and blood of Thoroughbred foals. Veterinary Journal 196, 269-271. Go to original source... Go to PubMed...
    16. Ranade R, Talukder S, Muscatello G, Celi P (2014): Assessment of oxidative stress biomarkers in exhaled breath condensate and blood of dairy heifer calves from birth to weaning. Veterinary Journal 202, 583-587. Go to original source... Go to PubMed...
    17. Rueda-Jasso R, Conceicao LEC, Dias J, De Coen W, Gomes E, Rees JF, Soares F, Dinis MT, Sorgeloos, P (2004): Effect of dietary non-protein energy levels on condition and oxidative status of Senegalese sole (Solea senegalensis) juveniles. Aquaculture 231, 417-433. Go to original source...
    18. Sanchez-Muros MJ, Villacreces S, Miranda-de la Lama G, de Haro C, Garcia-Barroso F (2013): Effects of chemical and handling exposure on fatty acids, oxidative stress and morphological welfare indicators in gilthead sea bream (Sparus aurata). Fish Physiology and Biochemistry 39, 581-591. Go to original source... Go to PubMed...
    19. Stohs SJ, Bagchi D, Hassoun E, Bagchi M (2000): Oxidative mechanisms in the toxicity of chromium and cadmium ions. Journal of Environmental Pathology, Toxicology and Oncology 19, 201-213.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content