Vet Med - Czech, 2016, 61(5):256-262 | DOI: 10.17221/8880-VETMED

Antimicrobial effects of curcumin against L. monocytogenes, S. aureus, S. Typhimurium and E. coli O157:H7 pathogens in minced meatOriginal Paper

S. Sandikci Altunatmaz1, F. Yilmaz Aksu1, G. Issa2, B. Basaran Kahraman3, D. Dulger Altiner4, S.K. Buyukunal5
1 Veterinary Faculty, Vocational High School, Istanbul University, Avcilar, Istanbul, Turkey
2 Avrupa Vocational School, Zeytinburnu, Istanbul, Turkey
3 Veterinary Faculty, Istanbul University, Avcilar, Istanbul, Turkey
4 Istanbul Aydin University, Besyol, Istanbul, Turkey
5 School of Health Sciences, Arel University, Istanbul, Turkey

The aim of this study was to determine the antimicrobial efficacy of curcumin, one of the active components of the Curcuma longa (turmeric) plant, against food pathogens in a minced meat medium. Salmonella Typhimurium ATCC 14028, Listeria monocytogenes ATCC 7644, Escherichia coli O157:H7 ATCC 33150 and S. aureus ATCC 25923 strains were used as food pathogens. Minimum inhibitory concentrations (MICs) were determined using the macrodilution method. MIC values for curcumin were found to be 125 µg/ml for L. monocytogenes and S. aureus, and 250 µg/ml for S. Typhimurium and E. coli O157:H7. Food pathogens were added to the minced meat at 104 CFU/g (including the control group) and curcumin at doses of 0.5%, 1% and 2% (except the control). The curcumin-supplemented minced meat and control were analysed 0-7 days later. At the end of seven days, it was seen that the 2% dose of curcumin had lowered L. monocytogenes and S. aureus counts by approximately 3 log CFU/g, and E. coli O157:H7 and S. Typhimurium counts by approximately 2 log CFU/g; the 1% dose had lowered L. monocytogenes, S. aureus, E. coli O157:H7 and S. Typhimurium counts by approximately 2 log CFU/g; and that the 0.5% curcumin dose had lowered L. monocytogenes and S. aureus count by approximately 2 log CFU/g, and E. coli O157:H7 and S. Typhimurium count by approximately 1 log CFU/g. Changes in bacterial counts were found to be statistically significant (P ≤ 0.05). It was observed that antibacterial effect increased in direct proportion to dose, while sensory approval decreased. In this study, 0.5% and 1% curcumin doses were determined to be sensorily acceptable. It was concluded that, in view of the scientific benefits and antimicrobial efficacy of curcumin, it may be used instead of, or in smaller doses together with preservative additives in foods where colour change is not important.

Keywords: curcumin; antimicrobial effect; foodborne pathogen; minced meat

Published: May 31, 2016  Show citation

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Sandikci Altunatmaz S, Yilmaz Aksu F, Issa G, Basaran Kahraman B, Dulger Altiner D, Buyukunal SK. Antimicrobial effects of curcumin against L. monocytogenes, S. aureus, S. Typhimurium and E. coli O157:H7 pathogens in minced meat. Vet Med - Czech. 2016;61(5):256-262. doi: 10.17221/8880-VETMED.
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    References

    1. Abdollahzadeh E, Rezaei M, Hosseini H (2014): Antibacterial activity of plant essential oils and extracts: The role of thyme essential oil, nisin, and their combination to control Listeria monocytogenes inoculated in minced fish meat. Food Control 35, 177-183. Go to original source...
    2. Aggarwal BB, Sundaram C, Malani N, Ichikawa H (2007): Curcumin: the Indian solid gold. Advances in Experimental Medicine and Biology 595, 1-75. Go to original source... Go to PubMed...
    3. Akpolat M, Tarladacalisir TY, Uz YH, Metin SM, Kizilay G (2010): Effect of curcumin in cancer treatment. New Medical Journal 27, 142-147.
    4. Andrews HW, Jacobson A, Hammack T (2011): Salmonella. Bacteriological Analytical Manual, Chapter 5, FDA. Food Science Research, Laboratory Methods (http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm070149.htm).
    5. Bennett RW, Lancette GA (2001): S. aureus. Bacteriological Analytical Manual. 8th ed. Revision A, Chapter 12, 222-227.
    6. Bhawana, Basniwal RK, Buttar HS, Jain VK, Jain N (2011): Curcumin nanoparticles: Preparation, characterization, and antimicrobial study. Journal of Agricultural and Food Chemistry 59, 2056-2061. Go to original source... Go to PubMed...
    7. Chapman PA, Malo ATC, Ellin M, Ashton R, Harkin MA (2001): Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire, UK. International Journal of Food Microbiology 64, 139-150. Go to original source... Go to PubMed...
    8. CLSI - Clinical and Laboratory Standards Institute (2000): Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standards. CLSI document M7-A5. CLSI, Wayne, PA.
    9. Gokce R (2011): Preserving food with antimicrobial substances. In: Erkmen O (ed.): Food Microbiology. 3rd ed. Efil Publications, Ankara, Turkey. 234-247.
    10. Goncalves AC, Almeida RCC, Alves MAO, Almeida PF (2005): Quantitative investigation on the effects of chemical treatments in reducing Listeria monocytogenes populations on chicken breast. Food Control 16, 617-622. Go to original source...
    11. Gulcubuk A, Altunatmaz K, Sonmez K, Haktanir D, Uzun H, Gurel A, Aydin S (2006): Effects of curcumin on tumour necrosis factor-α and interleukin-6 in late phase of experimental acute pancreatitis. Journal of Veterinary Medicine Series A: Physiology Pathology Clinical Medicine 53, 49-54. Go to original source... Go to PubMed...
    12. Gunes H, Gulen D, Mutlu R, Gumus A, Tas T, Topkaya AE (2013): Antibacterial effects of curcumin: an in vitro minimum inhibitory concentration study. Toxicology and Industrial Health (http://tih.sagepub.com/content/early/2013/10/14/0748233713498458).
    13. Hitchins DA, Jinneman K (2011): Detection and enumeration of Listeria monocytogenes. Bacteriological Analytical Manual, Chapter 10, FDA, Food Science Research, Laboratory Methods (http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm071400.htm).
    14. Hosny IM, Kholy WI, Murad HA, Dairouty RK (2011): Antimicrobial activitiy of curcumin upon pathogenic microorganisms during manufacture and storage of novel style cheese 'Karishcum'. Journal of American Science 7, 611-618.
    15. Inglet GE, Peterson SC, Carriere CJ, Maneepun S (2005): Rheological, textural, and sensory properties of Asian noodles containing an oat cereal hydrocolloid. Food Chemistry 90, 1-8. Go to original source...
    16. Kotzekidou P, Giannakidis P, Boulamatsis A (2008): Antimicrobial activity of some plant extracts and essential oils against foodborne pathogens in vitro and on the fate of inoculated pathogens in chocolate. LWT-Food Science and Technology 41, 119-127. Go to original source...
    17. Koyuncu I, Yildirim, I, Duranoglu S (2008): Antimicrobial feature of medical and aromatic herbals. In: 10th Turkish Food Congress Book, 21-23 May, Erzurum, Turkey, 913-916.
    18. Kurt S, Zorba O (2005): Bacteriocins and their potential usage in food. Journal of the Faculty of Veterinary Medicine University of Yuzuncu Yil 16, 77-83.
    19. Lourenco TC, Mendoca EP, Nalevaiko PC, Melo RT, Silva PL, Rossi DA (2013): Antimicrobial effect of turmeric (Curcuma longa) on chicken breast meat contamination. Brazilian Journal of Poultry Science 15, 79-82. Go to original source...
    20. McFarland J (1907): Nephelometer an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. Journal of the American Medical Association 14, 11761­ 178. Go to original source...
    21. Natta L, Orapin K, Krittika N, Pantip B (2008): Essential oil five Zingiberaceae for anti-food-borne bacteria. International Food Research Journal 15, 337-346.
    22. Negi PS (2012): Plant extracts fort the control of bacterial growth: Efficacy, stability and safety issues for food application. International Journal of Food Microbiology 156, 7-17. Go to original source... Go to PubMed...
    23. Ozcan M, Akgul A (1995): Natural colourants for food-II. Gida 20, 365-369.
    24. Pattaratanawadee E, Rachtanapun C, Wanchaitanawong W (2006): Antimicrobial activity of spice extracts againist pathogenic and spoilage microorganisms. Kasetsart Journal: Natural Science 4, 159-165.
    25. Sharma RA, Gescher AJ, Steward WP (2005): Curcumin: The story: so far. Euopean Journal of Cancer 41, 1955-1968. Go to original source... Go to PubMed...
    26. Sireli UT, Goncuoglu M, Pehlivanlar S (2008): Growth of Listeria monocytogenes in raw meat ball. Growth of Listeria monocytogenes in raw meat ball. Veterinary Journal of Ankara University 55, 83-87.
    27. Solomakos N, Govaris A, Koidis P, Botsoglou N (2008): The antimicrobial effect of thyme essential oil, nisin and their combination against Escherichia coli O157:H7 in minced beef during refrigerated storage. Meat Science 80, 159-166. Go to original source... Go to PubMed...
    28. Stankovic I (2014): Chemical and Technical Assessment (CTA). FAO, 61st JECFA.
    29. Tajbakhsh S, Mohammadi K, Deilami I, Zandi K, Fouladvand M, Ramedani E, Asayesh G (2008): Antibacterial activity of indium curcumin and indium diacetlycurcumin. African Journal of Biotechnology 7, 3832-3835.
    30. Wang Y, Lu Z, Wu H, Lv F (2009): Study on the antibiotic of microcapsule curcumin againist foodborne pathogens. International Journal of Food Microbiology 136, 71-74. Go to original source... Go to PubMed...
    31. Wang FY, Zhang C, Lu ZX (2012): Food preservation effects of curcumin microcapsules. Food Control 27, 112-117. Go to original source...

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