Vet Med - Czech, 2016, 61(6):308-316 | DOI: 10.17221/169/2015-VETMED

Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activityOriginal Paper

H. Akkari1, F. B'chir2, S. Hajaji1, M. Rekik3, E. Sebai1, H. Hamza1, M.A. Darghouth1, M. Gharbi1
1 National School of Veterinary Medicine, University of Manouba, Sidi Thabet, Tunisia
2 Institut National de Recherche et d'Analyse Physico-Chimique-Pole Technologique de Sidi Thabet, Sidi Thabet, Tunisia
3 International Center for Agricultural Research in the Dry Areas (ICARDA), Amman, Jordan

Capparis spinosa is cultivated for several proprieties and the different parts of the plant (flower buds, fruits, leaf and seeds) have been employed in drugs, foods and cosmetics. This study aimed to explore the antioxidant potential effect and to assess the anthelmintic efficacy of an aqueous extract of Capparis spinosa. In vitro antioxidant activity of aqueous extracts from the fresh leaves and flower buds of C. spinosa was measured by determining free radical-scavenging activity against DPPH and ABTS radical cations. In vitro anthelmintic activities against eggs and adult worms of Haemonchus contortus from sheep were investigated in comparison to albendazole. DPPH and ABTS activities of both aqueous extracts increased in a dose dependent manner. The IC50 values of aqueous extracts from fresh leaves, flowers buds and ascorbic acid in the DPPH radical scavenging assay were 101.40; 70.40 and 57.56 μg/ml, respectively. The IC50 values of aqueous extracts fresh leaves, flowers buds and ascorbic acid in the ABTS radical scavenging assay were 110; 87.89 and 58.17 μg/ml, respectively. Both plant extracts showed ovicidal activity at all tested concentrations. The aqueous extract from flower buds (IC50 = 2.76 mg/ml) showed higher inhibitory effects (P < 0.05) than aqueous extract from leaves (IC50 = 8.54 mg/ml) in the egg hatching assay. The aqueous extract from flower buds inhibited more worms than the aqueous extract from leaves at all tested concentrations. After 6 h of exposure, the highest tested concentrations of aqueous extracts from flower buds and leaves induced 100 and 41.66% inhibition of motility, respectively. These results show for the first time that C. spinosa possesses in vitro anthelmintic properties which may be related to its high content of phenolic compounds such as flavonoids and tannins.

Keywords: Capparis spinosa; free radical-scavenging ability; anthelmintic; Haemonchus contortus

Published: June 30, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Akkari H, B'chir F, Hajaji S, Rekik M, Sebai E, Hamza H, et al.. Potential anthelmintic effect of Capparis spinosa (Capparidaceae) as related to its polyphenolic content and antioxidant activity. Vet Med - Czech. 2016;61(6):308-316. doi: 10.17221/169/2015-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdul Ameer AA (2016): Assessment of the antioxidant properties of the caper fruit (Capparis spinosa L.) from Bahrain. Journal of the Association of Arab Universities for Basic and Applied Sciences 19, 1-7. Go to original source...
    2. Akkari H, Darghouth MA, Ben Salem H (2008a): Preliminary investigation of the anti-nematode activity of Acacia cyanophylla Lindl.: excretion of gastrointestinal nematode eggs in lambs browsing A. cyanophylla with and without PEG or grazing native grass. Small Ruminant Research 74, 78-83. Go to original source...
    3. Akkari H, Ben Salem H, Gharbi M, Abidi S, Darghouth MA (2008b): Feeding Acacia cyanophylla Lindl. foliage to Barbarine lambs with or without PEG: Effect on the excretion of gastro-intestinal nematode eggs. Animal Feed Science and Technology 147, 182-192. Go to original source...
    4. Akkari H, Rtibi K, B'chir F, Rekik M, Darghouth MA, Gharbi M (2014): In vitro evidence that the pastoral Artemisia campestris species exerts an anthelmintic effect on Haemonchus contortus from sheep. Veterinary Research Communications 38, 249-255. Go to original source... Go to PubMed...
    5. Alawa CB, Adamu AM, Getu JO, Ajansu, OJ, Abdu PA, Chiezey NP, Alawa JN, Bowman DD (2003): In vitro screening of two Nigeria medicinal plants (Vernonia amiygdalina and Annona senegalensis) for anthelmintic activity. Veterinary Parasitology 113, 73-81. Go to original source... Go to PubMed...
    6. Ali-Shtayeh MS, Abu Ghdeib SI (1999): Antifungal activity of plant extracts against dermatophytes. Mycoses 42, 665-672. Go to original source... Go to PubMed...
    7. Aliyazicioglu R, Eyupoglu OE, Sahin H, Yildiz O, Baltas N (2013): Phenolic components, antioxidant activity, and mineral analysis of Capparis spinosa L. African Journal of Biotechnology 12, 6643-6649. Go to original source...
    8. Arena A, Bisignano G, Pavone B, Tomaino A, Bonina FP, Saija A, Cristani M, D'Arrigo M, Trombetta D (2008): Antiviral and immunomodulatory effect of a lyophilized extract of Capparis spinosa L. buds. Phytotherapy Research 22, 313-317. Go to original source... Go to PubMed...
    9. Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, Vasseur J, Cazin M, Cazin JC, Pinkas M (1996): Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-Forschung 46, 1086-1089.
    10. Bashir AL, Chishti MZ, Fayaz AB, Hidayatullah T, Suhaib AB (2012): In vitro and in vivo anthelmintic activity of Euphorbia helioscopia L. Veterinary Parasitology 189, 317-321. Go to original source... Go to PubMed...
    11. Calderon-Montana JM, Burgos-Moron E, Perez-Guerrero C, Lopez-Lazaro M (2011): A review on the dietary flavonoid Kaemferol. Mini-Reviews in Medicinal Chemistry 11, 298-344. Go to original source... Go to PubMed...
    12. Coles GC, Bauer C, Borgsteede FHM, Geerts S, Taylor MA, Waller PJ (1992): World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology 44, 35-44. Go to original source... Go to PubMed...
    13. Cuenod A, Pottier-Alapetite G, Labbe A (1954): Analytic and synoptic flora of Tunisia: Cryptogames vasculaires, Gymnospermes et Monocotyledones (in French). Office de l'Experimentation et de la Vulgarisation Agricoles de Tunisie, Tunis. 287 pp.
    14. Deeba F, Muhammad G, Iqbal ZI (2009): Survey of ethnoveterinary practices used for different ailments in dairy animals in periurban areas of Faisalabad (Pakistan). International Journal of Agriculture and Biology 11, 535- 541.
    15. Dhar DN, Sharma RL, Bansal GC (1982): Gastrointestinal nematodes in sheep in Kashmir. Veterinary Parasitology 11, 271-277. Go to original source... Go to PubMed...
    16. Eddouks M, Lemhadri A, Michel JB (2004): Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats. Journal of Ethnopharmacology 94, 143-148. Go to original source... Go to PubMed...
    17. Eddouks M, Lemhadri A, Michel JB (2005): Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. Journal of Ethnopharmacology 98, 345-350. Go to original source... Go to PubMed...
    18. Elandolsi RB, Akkari H, Bchir F, Gharbi M, Mhadbi M, Awedi S, Darghouth MA (2013): Thymus capitatus from Tunisian arid zone: chemical composition and in vitro anthelmintic effects on Haemonchus contortus. Veterinary Parasitology 197, 374-378. Go to original source... Go to PubMed...
    19. Ferreira JFS (2011): Artemisia species in small ruminant production: their potential antioxidant and anthelmint i c ef fe ct s. Av ailable from:www.ddr.nal.usda.gov/ bistream/10113/42326/1/IND44379380.pdf.
    20. Gorinstein S, Haruenkit R, Park YS, Jung ST, Zachwieja Z, Jastrzebski Z, Katrich E, Trakhtenberg S, Belloso O (2004): Bioactive compounds and antioxidant potential in fresh and dried Jaffar sweeties, a new kind of citrus fruit. Journal of the Science of Food and Agriculture 84, 1459-1463. Go to original source...
    21. Hasanuzzaman M, Ali MR, Hossain, M, Kuri S, Islam MS (2013): Evaluation of total phenolic content, free radical scavenging activity and phytochemical screening of different extracts of Averrhoa bilimbi (fruits). International Current Pharmaceutical Journal 2, 92-96. Go to original source...
    22. Hoste H, Torres-Acosta JF, Paolini V, Aguilar-Caballero A, Etter E, Lefrileux Y, Chartier C, Broqua C (2005): Interactions between nutrition and gastrointestinal infections with parasitic nematodes in goats. Small Ruminant Research 60, 141-151. Go to original source...
    23. Hounzangbe-Adote MS, Paolni V, Fouraste I, Moutairou K, Hoste H (2005): In vitro effects of four tropical plants on three life-cycle stages of the parasitic nematode, Haemonchus contortus. Research in Veterinary Science 2, 155-160. Go to original source... Go to PubMed...
    24. Iqbal Z, Lateef M, Ashraf M, Jabbar A (2004): Anthelmintic activity of Artemisia brevifolia in sheep. Journal of Ethnopharmacology 93, 265-268. Go to original source... Go to PubMed...
    25. Jacobson RL, Schlein Y (1999): Lectins and toxins in the plant diet of Phlebotomus papatasi (Diptera: Psychodidae) can kill Leishmania major promastigotes in the sandfly and in culture. Annals of Tropical Medicine and Parasitology 93, 351-356. Go to original source...
    26. Klongsiriwet C, Quijada J, Williams AR, Mueller-Harvey I, Williamson EM, Hoste H (2015): Synergistic inhibition of Haemonchus contortus exsheathment by flavonoid monomers and condensed tannins. International Journal for Parasitology - Drugs and Drug Resistance 5, 127-134. Go to original source... Go to PubMed...
    27. Landau SY, Muklada H, Abu-Rabia A, Kaadan S, Azaizeh H (2014): Traditional Arab ethno-veterinary practices in small ruminant breeding in Israel. Small Ruminant Research 119, 161-171. Go to original source...
    28. Lasisi A, Kareem SO (2011): Evaluation of anthelmintic activity of stem bark extract and chemical constituents of Bridelia ferruginae (Benth) Euphorbiaceae. African Journal of Plant Science 5, 469-474.
    29. Mahasneh AM (2002): Screening of some indigenous Qatari medicinal plants for antimicrobial activity. Phytotherapy Research 16, 751-753. Go to original source... Go to PubMed...
    30. Molan AL, Meagher LP, Spencer PA, Sivakumaran S (2003): Effect of flavan-3-ol on in vitro egg hatching, larval development and viability of infective larvae of Trichostrongylus colubriformis. International Journal of Parasitology 33, 1691-1698. Go to original source... Go to PubMed...
    31. Panico AM, Cardile V, Garufi F, Puglia C, Bonina F, Ronsisvalle G (2005): Protective effect of Capparis spinosa on chondrocytes. Life Sciences 77, 2479-2488. Go to original source... Go to PubMed...
    32. Paria S, Maity S, Mookerjee M (2012): Phytochemial investigation and evaluation of anthelmintic activities of V. negundo leaf extract. International Journal Research Pharmaceutical Biomedical Sciences 3, 1143-1146.
    33. Parveen N (1991): Antifilarial activity of Vitex negundo against Setaria cervi. Fitoterapia 62, 163-165.
    34. Shaik YB, Castellani ML, Perrella A, Conti F, Salini V, Tete S, Madhappan B, Vecchiet J, De Lutiis MA, Caraffa A, Cerulli G (2006): Role of Quercitin (a natural herbal compound) in allergy and inflammation. Journal of Biological Regulators and Homeostatic Agents 20, 47-52.
    35. Siddhuraju P (2006): The antioxidant activity and free radical-scavenging capacity of phenolics of raw and dry heated moth bean (Vigna aconitifolia) (Jacq.) Marechal seed extracts. Food Chemistry 99, 149-157. Go to original source...
    36. Sindhu ZUD, Iqbal Z, Khan MN, Jonsson NN, Siddique M (2010): Documentation of ethno-veterinary practices used for treatment of different ailments in a selected hilly area of Pakistan. International Journal of Agriculture and Biology 12, 353-358.
    37. Singleton VL, Orthofe R, Lamuela-Raventos RM (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Oxidants and Antioxidants 299, 152-178. Go to original source...
    38. Soyler D, Khawar KM (2007): Seed germination of caper (Capparis ovate var. Herbace) using, naphthalene acetic acid and gibberellic acid. International Journal of Agriculture and Biology 9, 35-37.
    39. Sun BS, Leandro MC, Ricardo-da-Silva JM, Spranger MI (1998): Separation of grape and wine proanthocyanidins according to their degree of polymerization. Journal of Agricultural and Food Chemistry 46, 1390-1396. Go to original source...
    40. Terrill TH, Dykes GS, Shaik SA, Miller JE, Kouakou B, Kannan G, Burke JM, Mosjidis JA (2009): Efficacy of Sericea lespedeza hay as a natural dewormer in goats: Dose titration study. Veterinary Parasitology 163, 52-56. Go to original source... Go to PubMed...
    41. Trombetta D, Occhiuto F, Perri D, Puglia C, Santagati NA, De Pasquale A, Saija A, Bonina F (2005): Antiallergic and antihistaminic effect of two extracts of Capparis spinosa L. flowering buds. Phytotherapy Research 19, 29-33. Go to original source... Go to PubMed...
    42. Van Wyk JA, Stenson MO, Van der Merwe JS, Vorster RJ, Viljoen PG (1999): Anthelmintic resistance in South Africa: surveys indicate an extremely serious situation in sheep and goat farming. Onderstepoort Journal of Veterinary Research 66, 273-284.
    43. Waller PJ (1997): Sustainable helminth control of ruminants in developing countries. Veterinary Parasitology 71, 195-207. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content