Vet Med - Czech, 2016, 61(12):663-668 | DOI: 10.17221/45/2016-VETMED

Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern TurkeyOriginal Paper

H. Kalender1, A. Kilic2
1 Department of Bioengineering, University of Firat, Elazig, Turkey
2 Sivrice Vocational High School, University of Firat, Elazig, Turkey

Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an important foodborne pathogen worldwide. In Turkey, little is known about the molecular characteristics of STEC O157:H7 isolates. The aim of this study was to examine the genetic diversity of STEC O157:H7 isolates from cattle in Turkey using pulsed-field gel electrophoresis (PFGE). A total of 18 STEC O157:H7 strains were isolated from 540 rectal swab samples of slaughtered cattle in two abattoirs (coded A and B) located in eastern Turkey. The presence of virulence genes (stx1, stx2, hlyA and eae) was detected using the polymerase chain reaction. Of the 18 STEC O157:H7 isolates, 14 had stx2, hlyA, and eae genes. Four of the isolates were positive for stx1, stx2, hlyA, and eae genes. Thirteen different PFGE patterns were observed among the STEC O157:H7 isolates. The most common PFGE pattern was X1, which was detected in six STEC O157:H7 isolates. These six isolates contained the same virulence genes (stx2, eae and hlyA). However, some of the isolates containing the same virulence genes showed different PFGE profiles. Eleven different PFGE patterns were detected among the isolates from abattoir B, and two different PFGE patterns were detected among the isolates from abattoir A. The results of PFGE revealed a wide genetic diversity among the STEC O157:H7 isolates from cattle in Turkey. This is the first study on PFGE typing of STEC O157:H7 isolates from cattle in Turkey and further studies are needed.

Keywords: pulsed-field gel electrophoresis; virulence genes; bovine; rectal swabs; E. coli strains

Published: December 31, 2016  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kalender H, Kilic A. Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey. Vet Med - Czech. 2016;61(12):663-668. doi: 10.17221/45/2016-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Amezquita-Lopez BA, Quinones B, Cooley MB, Leon-Felix J, Campo NC, Mandrel RE, Jimenez M, Chaidez C (2012): Genotypic analyses of Shiga toxin-producing Escherichia coli O157 and non-O157 recovered from faeces of domestic animals on rural farms in Mexico. PLoS One 7, 1-12. Go to original source... Go to PubMed...
    2. Bono JL, Smith TPL, Keen JE, Harhay GP, McDaneld TG, Mandrell RE, Jung WK, Besser TE, Gerner-Smidt P, Bielaszewska M, Karck H, Clawson ML (2012): Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans. Molecular Biology and Evolution 29, 2047-2062. Go to original source... Go to PubMed...
    3. Chui L, Li V, Fach P, Delannoy S, Malejczyk K, PattersonFortin L, Poon A, King R, Simmonds K, Scott AN, Lee MC (2015): Molecular profiling of Escherichia coli O157:H7 and non-O157 strains isolated from humans and cattle in Alberta, Canada. Journal of Clinical Microbiology 53, 986-990. Go to original source... Go to PubMed...
    4. Cobbaut K, Houf K, Haesebrouck, Zutter LD (2011): Genotyping and antimicrobial resistance patterns of Escherichia coli O157 originating from cattle farms. Foodborne Pathogens and Disease 8, 719-723. Go to original source... Go to PubMed...
    5. D'Astek BA, del Castillo LL, Miliwebsky E, Carbonari C, Palladino PM, Deza N, Chinen I, Manfredi E, Leotta GA, Masana MO, Rivas M (2012): Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina. Foodborne Pathogens and Disease 9, 457-464. Go to original source... Go to PubMed...
    6. DebRoy C, Roberts E (2006): Screening petting zoo animals for the presence of potentially pathogenic Escherichia coli. Journal of Veterinary Diagnostic Investigation 18, 597-600. Go to original source... Go to PubMed...
    7. Donaldson SC, Straley BA, Hegde NV, Swant AA, DebRoy C, Jayarao BM (2006): Molecular epidemiology of ceftiofur-resistant Escherichia coli isolates from dairy calves. Applied and Environmental Microbiology 72, 3940-3948. Go to original source... Go to PubMed...
    8. Erdogan H, Erdogan A, Levent B, Kayali R, Arslan H (2008): Enterohaemorrhagic Escherichia coli O157:H7: case report. The Turkish Journal of Pediatrics 50, 488-491.
    9. Eriksson E, Soderlund R, Boqvist S, Aspan A (2010): Genotypic characterization to identify markers associated with putative hypervirulence in Swedish Escherichia coli O157:H7 cattle strains. Journal of Applied Microbiology 110, 323-332. Go to original source... Go to PubMed...
    10. Ferreira MRA, Filho EGF, Pinto JFN, Dias M, Moreira CN (2014): Isolation, prevalence and risk factors for infection by Shiga toxin-producing Escherichia coli (STEC) in dairy cattle. Tropical Animal Health and Production 46, 635- 639. Go to original source... Go to PubMed...
    11. Gauntom RK (1997): Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. Journal of Clinical Microbiology 35, 2977-2980. Go to original source... Go to PubMed...
    12. Hiroi M, Takahashi N, Harada T, Kawamori F, Iada N, Kanda T, Sugiyama K, Ohashi N, Hara-Kudo Y, Masuda T (2012): Serotype, Shiga toxin (stx) type and antimicrobial resistance of stx-producing Escherichia coli isolated from humans in Shizuoka Prefecture, Japan (2003-2007). Japanese Journal of Infectious Diseases 65, 198-202. Go to original source...
    13. Hussein HS, Bollinger LM (2005): Prevalence of Shiga toxinproducing Escherichia coli in beef cattle. Journal of Food Protection 68, 2224-2241. Go to original source... Go to PubMed...
    14. Jacob M, Almes KM, Shi X, Sargeant JM, Nagaraj TG (2011): Escherichia coli O157:H7 genetic diversity in bovine samples. Journal of Food Protection 74, 1186-1188. Go to original source... Go to PubMed...
    15. Kalender H (2013): Isolation, virulence genes and antimicrobial susceptibilities of Shiga toxin-producing Escherichia coli O157 from slaughtered cattle in abattoirs and ground beef sold in Elazig. Kafkas Universitesi Veteriner Fakultesi Dergisi 19, 461-467. Go to original source...
    16. Kawano K, Ono H, Iwashita O, Kurogi M, Haga T, Maeda K, Goto Y (2012): Stx genotype and molecular epidemiological analyses of Shiga toxin-producing Escherichia coli O157:H7/H- in human and cattle isolates. European Journal of Clinical Microbiology and Infectious Diseases 31, 119-127. Go to original source... Go to PubMed...
    17. Kuyucuoglu Y, Seker E, Sareyyupoglu B, Gurler Z (2011): Virulence genes of Shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle. Ankara Universitesi Veteriner Fakultesi Dergisi 58, 255-260. Go to original source...
    18. Lenahan ML, O'Brien SB, Byrne C, Ryan M, Kennedy CA, McNamara EB, Fanning S, Sheridan JJ, Sweeney T (2009): Molecular characterization of Irish E. coli O157:H7 isolates of human, bovine, ovine and porcine origin. Journal of Applied Microbiology 107, 1340-1349. Go to original source... Go to PubMed...
    19. Liebana E, Smith RP, Lindsay E, McLaren I, Cassar C, Clifton-Hadley FA, Paiba A (2003): Genetic diversity among Escherichia coli O157:H7 isolates from bovines living on farms in England and Wales. Journal of Clinical Microbiology 41, 3857-3860. Go to original source... Go to PubMed...
    20. Money P, Kelly AF, Gould SWJ, Denholm-Price J, Threfall EJ, Fielder MD (2010): Cattle, weather and water: mapping Escherichia coli O157:H7 infections in humans in England and Scotland. Environmental Microbiology 12, 2633-2644. Go to original source... Go to PubMed...
    21. Mora A, Blanco M, Blanco JE, Alanso MP, Dhabi G, Thomson-Carter F, Usera MA, Bartolome R, Prats G, Blanco J (2004): Phage types and genotypes of Shiga toxin-producing Escherichia coli O157:H7 isolates from humans and animals in Spain: identification and characterization of two predominating phage types (PT2 and PT8). Journal of Clinical Microbiology 42, 4007-4015. Go to original source... Go to PubMed...
    22. Posse B, De Zutter L, Heyndrickx M, Herman L (2007): Metabolic and genetic profiling of clinical O157 and nonO157 Shiga-toxin-producing Escherichia coli. Research in Microbiology 158, 591-599. Go to original source... Go to PubMed...
    23. Prendergast DM, Lendrum L, Pearce R, Ball C, McLernon J, O'Grady D, Scott L, Fanning S, Egan J, Gutierrez M (2011): Verocytotoxigenic Escherichia coli O157 in beef and sheep abattoirs in Ireland and characterization of isolates by pulsed-field gel electrophoresis and multilocus variable number of tandem repeat analysis. International Journal of Food Microbiology 144, 519-527. Go to original source... Go to PubMed...
    24. Sanchez S, Martinez R, Rey J, Garcia A, Blanco J, Blanco M, Blanco JE, Mora A, Herrera-Leon S, Echeita A, Alonso JM (2010): Pheno-genotypic characterisation of Escherichia coli O157:H7 isolates from domestic and wild ruminants. Veterinary Microbiology 142, 445-449. Go to original source... Go to PubMed...
    25. Scott L, McGee P, Minihan D, Sheridan JJ, Earley B, Leonard N (2006): The characterization of Escherichia coli O157:H7 isolates from cattle faeces and feedlot environment using PFGE. Veterinary Microbiology 114, 331-336. Go to original source... Go to PubMed...
    26. Wieler LH, Vieler E, Erpenstein C, Schlapp T, Steinruck H, Bauerfeind R, Byomi A, Baljer G (1996): Shiga toxinproducing Escherichia coli strains from bovines: Association of adhesion with carriage of eae and other genes. Journal of Clinical Microbiology 34, 2980-2984. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content