Vet Med - Czech, 2017, 62(2):53-61 | DOI: 10.17221/7/2016-VETMED

Dietary bacteriophages as an alternative for zinc oxide or organic acids to control diarrhoea and improve the performance of weanling pigletsOriginal Paper

A.R. Hosseindoust1, S.H. Lee1, J.S. Kim2, Y.H. Choi1, H.S. Noh1, J.H. Lee3, P.K. Jha1, I.K. Kwon1, B.J. Chae*,1
1 College of Animal Life Sciences, Kangwon National University, Chuncheon, Republic of Korea
2 Southern Research and Outreach Center, Minnesota, USA
3 CTCBIO Inc., Seoul, Republic of Korea

In this study, the antibacterial substances ZnO, organic acids and a bacteriophage cocktail were added to the diet of weanling pigs to evaluate the effects on gut health. Dietary treatments were basal diet without any supplementation (Control) and basal diet either supplemented with 0.34% (2500 ppm) ZnO, 0.20% organic acids or with 0.10% bacteriophage cocktail. Faecal score was decreased in ZnO and bacteriophage cocktail treatments. The total number of ileal anaerobic bacteria, Bifidobacterium spp. and Lactobacillus spp. were higher in ZnO, bacteriophage cocktail and organic acids, while ileal coliforms and caecal Clostridium spp. were decreased in comparison to Control. Faecal coliforms (Day 7 and Day 21) and Clostridium spp. (Day 21 and Day 35) were lower in bacteriophage cocktail. The gain to feed ratio was improved in all supplemented groups. The digestibility of dry matter was increased at the end of the experiment in all supplemented groups, while that of crude protein was increased only at Day 21 in bacteriophage cocktail. Duodenal villus height was increased in ZnO and bacteriophage cocktail. Bacteriophage cocktail also showed a greater villus height in the small intestine. Supplementation of bacteriophage cocktail in weaning pig diets resulted in better growth performance, digestibility and gut development compared to Control, and thus, it can be concluded that its effects are comparable to ZnO or organic acids supplementation.

Keywords: growth performance; intestinal morphology; microbiota; faecal score; coliforms; Clostridium

Published: February 28, 2017  Show citation

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Hosseindoust AR, Lee SH, Kim JS, Choi YH, Noh HS, Lee JH, et al.. Dietary bacteriophages as an alternative for zinc oxide or organic acids to control diarrhoea and improve the performance of weanling piglets. Vet Med - Czech. 2017;62(2):53-61. doi: 10.17221/7/2016-VETMED.
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    References

    1. AOAC - Association of Official Analytical Chemists (2007): Official Methods of Analysis of AOAC International. 18th edn. AOAC International. Gaithersburg, USA.
    2. Barbara G, Stanghellini V, Brandi G, Cremon C, Di Nardo G, De Giorgio R, Corinaldesi R (2005): Interactions between commensal bacteria and gut sensorimotor function in health and disease. American Journal of Gastroenterology 100, 2560-2568. Go to original source... Go to PubMed...
    3. Botermans JA, Svendsen MJ, Svendsen LS, Pierzynowski SG (1999): The exocrine pancreas in pig growth and performance. In: Pierzynowksi G and Zabielski R (eds): Biology of the Pancreas in Growing Animals. Elsevier Science, Amsterdam. 395-408.
    4. Carlson MS, Hill GM, Link JE (1999): Early and traditionally weaned nursery pigs benefit from phase feeding pharmacological concentrations of zinc oxide: effect on metallothionein and mineral concentrations. Journal of Animal Science 77, 1199-1201. Go to original source... Go to PubMed...
    5. Cengiz O, Koksal BH, Tatli O, Sevim O, Avci H, Epikmen T, Beyaz D, Buyukyoruk S, Boyacioglu M, Uner A, Onol AG (2012): Influence of dietary organic acid blend supplementation and interaction with delayed feed access after hatch on broiler growth performance and intestinal health. Veterinarni Medicina 57, 515-528. Go to original source...
    6. Chaveerach P, Keuzenkamp DA, Urlings HA, Lipman LJA, Knapen FV (2002): In vitro study on the effect of organic acids on Campylobacter jejuni/E. coli populations in mixtures of water and feed. Poultry Science 81, 621-628. Go to original source... Go to PubMed...
    7. Commission Regulation (EC) No. 1334/2003 of 25 July 2003 amending the conditions for authorization of a number of additives in feeding stuffs belonging to the group of trace elements. Official Journal of the European Union 187, 11-15.
    8. Cummings JH, Macfarlane GT (1991): The control and consequences of bacterial fermentation in the human colon. Journal of Applied Bacteriology 70, 443-459. Go to original source... Go to PubMed...
    9. Fenton TW, Fenton M (1979): An improved procedure for the determination of chromic oxide in feed and feces. Canadian Journal of Animal Science 59, 631-663. Go to original source...
    10. Gauthier R (2002): Intestinal health, the key to productivity - the case of organic acids. IASA XXVII convencion ANECA-WPDC. Puerto Vallarta, Mexico. 1-14.
    11. Gebru E, Lee JS, Son JC, Yang SY, Shin SA, Kim B, Kim MK, Park SC (2010): Effect of probiotic, bacteriophage, or organic acid-supplemented feeds or fermented soybean meal on the growth performance, acute-phase response, and bacterial shedding of grower pigs challenged with Salmonella enterica serotype Typhimurium. Journal of Animal Science 88, 3880-3886. Go to original source... Go to PubMed...
    12. Hampson DJ (1986): Alterations in piglet small intestinal structure at weaning. Research in Veterinary Science 40, 32-40. Go to original source...
    13. Herich R, Levkut M (2002): Lactic acid bacteria, probiotics and immune system. Veterinarni Medicina 47, 169-180. Go to original source...
    14. Hollis GR, Carter SD, Cline TR, Crenshaw TD, Cromwell GL, Hill GM, Kim SW, Lewis AJ, Mahan DC, Miller PS, Stein HH, Veum TL (2005): Effects of replacing pharmacological levels of dietary zinc oxide with lower dietary levels of various organic zinc sources for weanling pigs. Journal of Animal Science 83, 2123-2129. Go to original source... Go to PubMed...
    15. Hookman P, Barkin JS (2009): Clostridium difficile associated infection, diarrhoea and colitis. World Journal of Gastroenterology 15, 1554. Go to original source... Go to PubMed...
    16. Jamalludeen N, Johnson RP, Shewen PE, Gyles CL (2009): Evaluation of bacteriophages for prevention and treatment of diarrhea due to experimental enterotoxigenic Escherichia coli O149 infection of pigs. Veterinary Microbiology 136, 135-141. Go to original source... Go to PubMed...
    17. Kim YY, Kil DY, Oh HK, Han IK (2005): Acidifier as an alternative material to antibiotics in animal feed. Asian Australian Journal of Animal Science 18, 1048-1060. Go to original source...
    18. Kim KH, Ingale SL, Kim JS, Lee SH, Lee JH, Kwon IK, Chae BJ (2014): Bacteriophage and probiotics both enhance the performance of growing pigs but bacteriophage are more effective. Animal Feed Science and Technology 196, 88-95. Go to original source...
    19. Lambert RJ, Stratford M (1999): Weak acid preservatives: modeling microbial inhibition and response. Journal of Applied Microbiology 86, 157-164. Go to original source... Go to PubMed...
    20. Li BT, Van Kessel AG, Caine WR, Huang SX, Kirkwood RN (2001): Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide. Canadian Journal of Animal Science 81, 511-516. Go to original source...
    21. Llyod DAJ, Gabe SM (2008): Intestinal morphology, intestinal regeneration and the promise of tissue engineering. Intestinal Failure: Diagnosis, Management and Transplantation, 11-19. Go to original source...
    22. Macfarlane S, Macfarlane GT (2003): Regulation of short chain fatty acid production. Proceedings of the Nutrition Society 62, 67-72. Go to original source... Go to PubMed...
    23. MAFRA - Ministry of agriculture, food and rural affairs (2014): Control of livestock and fish feed act 46.
    24. Melin L, Mattsson S, Katouli M, Wallgren P (2004): Development of post-weaning diarrhoea in piglets. Relation to presence of Escherichia coli strains and rotavirus. Journal of Veterinary Medicine, Series B 51, 12-22. Go to original source... Go to PubMed...
    25. Mourao JL, Pinheiro V, Alves A, Guedes CM, Pinto L, Saavedra MJ, Spring P, Kocher A (2006): Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Animal Feed Science and Technology 26, 107-120. Go to original source...
    26. Mroz Z (2005): Organic acids as potential alternatives to antibiotic growth promoters for pigs. Advances in Pork Production 16, 169-182.
    27. NRC - National Research Council (2012): Nutrient Requirements of Swine. 11th edn. National Academy Press, Washington. 420 pp.
    28. Owusu-Asiedu A, Nyachoti CM, Marquardt RR (2003): Response of early-weaned pigs to an enterotoxigenic (K88) challenge when fed diets containing spray-dried porcine plasma or pea protein isolate plus egg yolk antibody, zinc oxide, fumaric acid, or antibiotic. Journal of Animal Science 81, 1790-1798. Go to original source... Go to PubMed...
    29. Partanen KH, Morz Z (1999): Organic acids for performance enhancement in pig diets. Nutrition Research Reviews 12, 117-145. Go to original source... Go to PubMed...
    30. Pluske JR, Hampson DJ, Williams IH (1997): Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livestock Production Science 51, 215-236. Go to original source...
    31. Salminen S, Salminen E (1997): Lactulose, lactic acid bacteria, intestinal microecology and mucosal protection. Scandinavian Journal of Gastroenterology 222, 45-48. Go to original source... Go to PubMed...
    32. Simon O (2005): Micro-organisms as feed additives-probiotics. Advance Pork Production 16, 161-167.
    33. Starr J (2005): Clostridium difficile associated diarrhoea: diagnosis and treatment. British Medical Journal 7515, 498-501. Go to original source... Go to PubMed...
    34. Suryanarayana MV, Suresh ANJ, Rajasekhar MV (2012): A study on the associated effect of probiotic and citric acid on the performance of pre-weaned piglets. Tamilnadu Journal of Veterinary Animal Science 8, 126-130.
    35. Thaela MJ, Jensen MS, Pierzynowski SG, Jakob S, Jensen BB (1998): Effect of lactic acid supplementation in pigs after weaning. Journal of Animal and Feed Sciences 7, 181-183. Go to original source...
    36. Trckova M, Lorencova A, Hazova K, Zajacova ZS (2015): Prophylaxis of post-weaning diarrhoea in piglets by zinc oxide and sodium humate. Veterinarni Medicina 60, 351-360. Go to original source...
    37. Upadhaya SD, Lee KY, Kim IH (2014): Influence of protected organic acid blends and diets with different nutrient densities on growth performance, nutrient digestibility and faecal noxious gas emission in growing pigs. Veterinarni Medicina 59, 491-497. Go to original source...
    38. Waern MJ, Melin L, Lindberg R, Johannisson A, Petersson L, Wallgren P (1998): Dietary zinc oxide in weaned pigs - effects on performance, tissue concentrations, morphology, neutrophil functions and fecal microflora. Research in Veterinary Science 64, 225-231. Go to original source... Go to PubMed...
    39. Walsh M, Sholly D, Kelly D, Cobb M, Trapp S, Hinson R, Hill B, Sutton A, Radcliffe S, Harmon B, Smith J, Richert B (2003): The effects of supplementing weanling pig diets with organic and inorganic acids on growth performance and microbial shedding. Purdue University, Swine Research Report, 89-98.
    40. Wittebole X, Roock SD, Opal SM (2014): A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens. Virulence 5, 1-10. Go to original source... Go to PubMed...
    41. Wu G, Meier SA, Knabe DA (1996): Dietary glutamine supplementation prevents jejunal atrophy in weaned pigs. Journal of Nutrition 126, 2578-2584. Go to original source... Go to PubMed...
    42. Yan L, Hong SM, Kim IH (2012): Effect of bacteriophage supplementation on the growth performance, nutrient digestibility, blood characteristics, and fecal microbial shedding in growing pigs. Asian-Australasian Journal of Animal Sciences 25, 1451-1456. Go to original source... Go to PubMed...
    43. Yoon JH, Ingale SL, Kim JS, Kim KH, Lee SH, Park YK, Kwon IK, Chae BJ (2012): Effects of dietary supplementation of antimicrobial peptide-A3 on growth performance, nutrient digestibility, intestinal and fecal microflora and intestinal morphology in weanling pigs. Animal Feed Science and Technology 177, 98-107. Go to original source...

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