Vet Med - Czech, 2017, 62(7):410-415 | DOI: 10.17221/174/2016-VETMED

Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech RepublicCase Report

J. Bzdil*,1, O. Holy2, J. Toporcak3
1 Department of Special Microbiology, State Veterinary Institute Olomouc, Olomouc, Czech Republic
2 Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic
3 Clinic of Birds, Exotic and Free Living Animals, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovak Republic

The aim of this study was to describe two case studies of reptile disease, in which the primary pathogen was apparently Salmonella enterica subspecies enterica serovar Paratyphi B. Pathological examinations, sampling from different organs and cloacal swabs was performed on a dead savannah monitor (Varanus exanthematicus) and a sick green tree python (Chondropython viridis). This material was subjected to culture examination, including selective enrichment using standard methods. Typing was performed using MALDI-TOF and strains were also serologically typified. The utilisation of d-tartrate was confirmed biochemically and also using PCR. Antibiotic susceptibility was determined by the standard disc diffusion method using Mueller-Hinton agar without blood and antibiotic discs. In both cases the detected Salmonella enterica subspecies enterica serovar Paratyphi B was positive for d-tartrate and exquisitely susceptible to chloramphenicol, tetracycline, ampicillin, amoxycillin/clavulanic acid, gentamicin, ceftazidime, enrofloxacin and piperacillin.

Keywords: paratyphoid; Varanus exanthematicus; Chondropython viridis; d-tartrate; treatment

Published: July 31, 2017  Show citation

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Bzdil J, Holy O, Toporcak J. Salmonella enterica subspecies enterica serovar Paratyphi B as a disease-causing agent in reptiles in the Czech Republic. Vet Med - Czech. 2017;62(7):410-415. doi: 10.17221/174/2016-VETMED.
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    References

    1. Bzdil J (2003): Determination of susceptibility of bacteria to antimicrobials by disc diffusion method (in Czech). Standard Operating Procedure of SVI Olomouc, 1-4.
    2. Bzdil J, Kubickova I, Kvapilova M (2012): Salmonella occurence in clinical material in human and veterinary sphere in period 2006-2010 (in Czech). Veterinarstvi 10, 625-629.
    3. Chart H (2003): The pathogenicity of strains of Salmonella paratyphi B and Salmonella java. Journal of Applied Microbiology 94, 340-348. Go to original source... Go to PubMed...
    4. Chart H, Cheasty T, de Pinna E, Siorvanes L, Wain J, Alam D, Nizami Q, Bhutta Z, Threlfall EJ (2007): Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections. Journal of Medical Microbiology 56, 1061-1066. Go to original source... Go to PubMed...
    5. Evans SJ, Davies RH, Binns SH, Liebana E, Jones TWH, Millar MF, Threlfall EJ, Ward LR, Hopkins KL, Mackay PHS, Gayford PJR (2005): Multiple antimicrobial resistant Salmonella enterica serovar Paratyphi B variant Java in cattle: a case report. Veterinary Record 156, 343-346. Go to original source... Go to PubMed...
    6. Hernandez E, Rodriguez JL, Herrera-Leon S, Garcia I, de Castro V, Muniozguren N (2012): Salmonella Paratyphi B var Java infections associated with exposure to turtles in Bizkaia, Spain, September 2010 to October 2011. Euro Surveillance 17, 6-10. Go to original source...
    7. Kauffmann F (1941): A typhoid variant and a new serological variation in the Salmonella group. Journal of Bacteriology 41, 127-140. Go to original source... Go to PubMed...
    8. Martinez-Urtaza J, Echeita A, Liebana E (2006): Phenotypic and genotypic characterization of Salmonella enterica serotype paratyphi B isolates from environmental and human sources in Galicia, Spain. Journal of Food Protection 69, 1280-1285. Go to original source... Go to PubMed...
    9. Miko A, Guerra B, Schroeter A, Dorn C, Helmuth R (2002): Molecular characterization of multiresistant d-tartratepositive Salmonella enterica serovar paratyphi B isolates. Journal of Clinical Microbiology 40, 3184-3191. Go to original source... Go to PubMed...
    10. Singh BR, Jyoti J, Chandra M, Babu N, Sharma G (2009): Drug resistance patterns of Salmonella isolates of equine origin from India. Journal of Infection in Developing Countries 3, 141-147. Go to original source... Go to PubMed...
    11. Stenglein MD, Sanders C, Kistler AL, Ruby JG, Franco JY, Reavill DR, Dunker F, De Risi JL (2012): Identification, characterization, and in vitro culture of highly divergent arenaviruses from boa constrictors and annulated tree boas: candidate etiological agents for snake inclusion body disease. mBio, doi: 10.1128/mBio.00180-12. Go to original source... Go to PubMed...
    12. Toboldt A, Tietze E, Helmuth R, Fruth A, Junker E, Malorny B (2012): Human infections attributable to the D-tartratefermenting variant of Salmonella enterica serovar Paratyphi B in Germany originate in reptiles and, on rare occasions, poultry. Applied and Environmental Microbiology 78, 7347-7357. Go to original source... Go to PubMed...

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