Vet Med - Czech, 2017, 62(12):668-673 | DOI: 10.17221/144/2016-VETMED

Heat-killed Tsukamurella inchonensis reduces lipopolysaccharide-induced inflammatory responses in activated murine peritoneal macrophagesOriginal Paper

K. Nofouzi*,1, M. Aghapour1, B. Baradaran2, G.H. Hamidian1, P. Zare1, J. Stanford3, P. Ripley4, K. Tahapour1, Y. Jafari1, A. Shahbazfar1, A. Tukmechi5
1 Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Centre for Infectious Diseases and International Health, Windeyer Institute of Medical Sciences, University College London, London, United Kingdom
4 BioEos Ltd., Mill House, Marden, United Kingdom
5 Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran

Tsukamurella inchonensis (T. inchonensis) is an aerobic species of Actinomycetales which has immunomodulatory activities when used as a suspension of killed bacilli. Here, the effects of T. inchonensis on lipopolysaccharide-induced inflammatory responses in mouse peritoneal macrophages have been examined. Peritoneal macrophages were harvested by lavaging with ice cold phosphate-buffered saline. Macrophages acquired from mice treated with different doses of T. inchonensis for seven days were cultured with 20 U/ml interferon-γ and 10 µg/ml lipopolysaccharide for in vivo assays. Nitrite levels were measured by using the diazotization method based on the Griess reaction, an indirect technique to determine nitric oxide (NO) production. T. inchonensis inhibited lipopolysaccharide-stimulated NO production in mouse peritoneal macrophages from mice previously exposed to concentrations of 108 and 5 × 107 CFU per flask. Also, T. inchonensis decreased lipopolysaccharide-induced production of pro-inflammatory cytokines, including interleukin-6 and tumor necrosis factor-α. Thus, it can be concluded that T. inchonensis is a powerful inhibitor of lipopolysaccharide-induced NO production in activated murine macrophages, and T. inchonensis may be useful as a novel agent for chemoprevention in inflammatory diseases.

Keywords: Suriyan mouse; actinomycetales; nitric oxide; tumor necrosis factor-α; TNF-α; interleukin-6; IL-6; chemoprevention

Published: December 31, 2017  Show citation

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Nofouzi K, Aghapour M, Baradaran B, Hamidian GH, Zare P, Stanford J, et al.. Heat-killed Tsukamurella inchonensis reduces lipopolysaccharide-induced inflammatory responses in activated murine peritoneal macrophages. Vet Med - Czech. 2017;62(12):668-673. doi: 10.17221/144/2016-VETMED.
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