Vet Med - Czech, 2018, 63(12):561-570 | DOI: 10.17221/63/2018-VETMED

The N and P genes facilitate pathogenicity of the rabies virus G geneOriginal Paper

X.K. Wei1,2, Y.Z. Zhong2, Y. Pan1,2, X.N. Li1,2, J.J. Liang1,2, T.R. Luo*,1,2
1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Guangxi, China
2 Laboratory of Veterinary Microbiology and Animal Infectious Diseases, Guangxi University, Guangxi, China

To explore the effects of different gene combinations on the pathogenicity of the rabies virus (RABV), six chimeric RABV mutants, rRC-HL(G), rRC-HL(NG), rRC-HL(PG), rRC-HL(NP), rRC-HL(NM) and rRC-HL(NPG), were constructed using a reverse genetic technique based on an avirulent parental rRC-HL strain and a virulent parental GX074 isolate. These mutants were intracerebrally inoculated into adult mice. The results indicated that 102 ffu and 106 ffu of rRC-HL(G), rRC-HL(NG), rRC-HL(PG) and rRC-HL(NPG) were 100% lethal. In the case of intramuscular viral infection, none of the mice inoculated with 102 ffu of any of the RABV mutants, including GX074, died; at 106 ffu, rRC-HL(G) was lethal in 2/5 cases, rRC-HL(NG) was lethal in 1/5 cases and rRC-HL(PG) was lethal for 2/5 mice. The rRC-HL(NPG) mutant was fatal for 3/5 mice, as was the parental GX074. Furthermore, the LD50 values of the chimeric RABV mutants were measured, with the results showing that the LD50 values of both rRC-HL(NG) and rRC-HL(PG) were lower than that of rRC-HL(G), but higher than that of rRC-HL(NPG). Thus, the action of N + G, or P + G, or N + P + G gene combinations may be more pronounced than that of the G gene alone. Body weight changes and the clinical symptoms of the tested mice were consistent with pathogenicity. These data indicate that the N and P genes are involved in and facilitate the pathogenicity of the RABV G gene. These experiments provide further evidence that multi-gene cooperation is responsible for the virulence of RABV.

Keywords: reverse genetics; gene combination; chimeric virus; lethality

Published: December 31, 2018  Show citation

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Wei XK, Zhong YZ, Pan Y, Li XN, Liang JJ, Luo TR. The N and P genes facilitate pathogenicity of the rabies virus G gene. Vet Med - Czech. 2018;63(12):561-570. doi: 10.17221/63/2018-VETMED.
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