Vet Med - Czech, 2020, 65(3):116-122 | DOI: 10.17221/82/2019-VETMED

In vitro pharmacokinetics/pharmacodynamics evaluation of marbofloxacin against Staphylococcus pseudintermediusOriginal Paper

Y Quah, N Boby, SC Park
Laboratory of Veterinary Clinical Pharmacology, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea

This study aimed at determining the in vitro antibacterial activity of a clinically achievable marbofloxacin (MAR) concentration against the clinical isolate S. pseudintermedius in an in vitro dynamic model simulating the in vivo pharmacokinetics of dogs. The in vitro PK/PD (pharmacokinetic/pharmacodynamic) model that mimics the single daily doses of MAR (half-life, 8 h) was simulated. An inoculum (108 cfu/ml) of clinical isolate S. pseudintermedius (MIC = 0.0625 μg/ml) was exposed to monoexponentially decreasing concentrations of MAR with simulated AUC24 h/MIC varied from 34.81 h to 696.15 h. Every two hours, the multiple sample colony forming units were determined. The result of this study demonstrated that the clinically achieved MAR concentrations at AUC24 h/MIC ratios of 348.08 and 696.15 h produced a pronounced reduction in the bacterial counts and prevented the re-growth of the clinical isolate S. pseudintermedius. However, further study, considering the strains with different susceptibility levels, is recommended.

Keywords: dogs; clinical isolates; simulation; antimicrobial resistance

Received: July 1, 2019; Accepted: November 26, 2019; Published: March 31, 2020  Show citation

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Quah Y, Boby N, Park S. In vitro pharmacokinetics/pharmacodynamics evaluation of marbofloxacin against Staphylococcus pseudintermedius. Vet Med - Czech. 2020;65(3):116-122. doi: 10.17221/82/2019-VETMED.
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