Vet Med - Czech, 2020, 65(6):250-257 | DOI: 10.17221/20/2019-VETMED

Quantitative analysis of CD4+CD25+FoxP3+ regulatory T-cells in canine atopic dermatitis in KoreaOriginal Paper

DJ Lee1, TH Chung2, C Park1
1 Department of Veterinary Internal Medicine, Jeonbuk National University, Jeonbuk, Republic of Korea
2 Division of Integrated Biotechnology, Joongbu University, Chungnam, Republic of Korea

Recently, it was suggested that CD4+CD25+FoxP3+ Tregs (Regulatory T-cells) exist in canine skin, although their numbers were not significantly different between healthy and atopic dogs. In this study, we investigated whether Treg frequencies correlate with the clinical features of canine atopic dermatitis (cAD). The goal of this study was to compare and analyse the numbers of the circulating Tregs in atopic and healthy dogs. In the peripheral blood mononuclear cells (PBMC) of healthy dogs, Tregs defined as CD4+CD25+FoxP3+ Tregs in the peripheral blood ranged from 0.3% to 1.5%. By contrast, in atopic dogs, the same population ranged from 0.7% to 8.8%. The percentage of CD4+CD25+FoxP3+ Tregs in gated CD4+ T-cells was significantly higher in the peripheral blood of dogs with atopic dermatitis (n = 9) than in the healthy controls (n = 8). The difference in the Treg levels (CD4+CD25+FoxP3+) (P = 0.012) between the atopic and the healthy groups was statistically significant. The circulating T-cells (phenotype CD4+CD25+FoxP3+ and CD4+FoxP3+) were increased significantly in the atopic dogs. The proportion of CD4+CD25+FoxP3+ Tregs of the atopic dogs decreased with advancing age. These findings suggest that changes in the Tregs may mediate the pathogenesis of CAD.

Keywords: canine; atopy; CD4+CD25+FoxP3+

Received: February 11, 2019; Accepted: December 2, 2019; Published: June 30, 2020  Show citation

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Lee D, Chung T, Park C. Quantitative analysis of CD4+CD25+FoxP3+ regulatory T-cells in canine atopic dermatitis in Korea. Vet Med - Czech. 2020;65(6):250-257. doi: 10.17221/20/2019-VETMED.
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    References

    1. Biller B, Elmslie R, Burnett R, Avery A, Dow S. Use of FoxP3 expression to identify regulatory T-cells in healthy dogs and dogs with cancer. Vet Immunol Immunopathol. 2007 Mar 15;116(1-2):69-78. Go to original source... Go to PubMed...
    2. Favrot C, Steffan J, Seewald W, Picco F. A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol. 2010 Feb;21(1):23-31. Go to original source... Go to PubMed...
    3. Gaspar K, Barath S, Nagy G, Mocsai G, Gyimesi E, Szodoray P, Irinyi B, Zeher M, Remenyik E, Szegedi A. Regulatory T-cell subsets with acquired functional impairment: Important indicators of disease severity in atopic dermatitis. Acta Derm Venereol. 2015 Feb;95(2):151-5. Go to original source... Go to PubMed...
    4. Hauck V, Hugli P, Meli ML, Rostaher A, Fischer N, Hofmann-Lehmann R, Favrot C. Increased numbers of FoxP3-expressing CD4+ CD25+ regulatory T-cells in peripheral blood from dogs with atopic dermatitis and its correlation with disease severity. Vet Dermatol. 2016 Feb;27(1):26-9. Go to original source... Go to PubMed...
    5. Ito Y, Adachi Y, Makino T, Higashiyama H, Fuchizawa T, Shimizu T, Miyawaki T. Expansion of FOXP3-positive CD4+CD25+ T-cells associated with disease activity in atopic dermatitis. Ann Allergy Asthma Immunol. 2009 Aug;103(2):160-5. Go to original source... Go to PubMed...
    6. Jassies-van der Lee A, Rutten VP, Bruijn J, Willemse T, Broere F. CD4+ and CD8+ skin-associated T lymphocytes in canine atopic dermatitis produce interleukin-13, interleukin-22 and interferon-γ and contain a CD25+ FoxP3+ subset. Vet Dermatol. 2014 Oct;25(5):456-72. Go to original source... Go to PubMed...
    7. Keppel K, Campbell K, Zuckermann F, Greeley E, Schaeffer D, Husmann R. Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy. Vet Immunol Immunopathol. 2008 Jun 15; 123(3-4):337-44. Go to original source... Go to PubMed...
    8. Knueppel A, Lange S, Sekora A, Altmann S, Freund M, Junghanss C. Phenotypic and functional characterization of freshly isolated and expanded canine regulatory Tcells. Exp Anim. 2011 Oct;60(5):471-9. Go to original source... Go to PubMed...
    9. Lesiak A, Smolewski P, Sobolewska-Sztychny D, Sysa-Jedrzejowska A, Narbutt J. The role of T-regulatory cells and Toll-like receptors 2 and 4 in atopic dermatitis. Scand J Immunol. 2012 Oct;76(4):405-10. Go to original source... Go to PubMed...
    10. Ling EM, Smith T, Nguyen XD, Pridgeon C, Dallman M, Arbery J, Carr VA, Robinson DS. Relation of CD4+ CD25+ regulatory T-cell suppression of allergen-driven T-cell activation to atopic status and expression of allergic disease. Lancet. 2004 Feb 21;363(9409):608-15. Go to original source... Go to PubMed...
    11. Navarro C, Crastes N, Benizeau E, McGahie D. Voluntary acceptance and consumption of two oral ciclosporin formulations in dogs: Two randomised, controlled studies. Ir Vet J. 2015 Feb 12;68(1):3. Go to original source... Go to PubMed...
    12. Olivry T, Marsella R, Pucheu-Haston CM, Hammerberg B. Mechanism of lesion formation in canine atopic dermatitis: 2004 hypothesis. In: Hillier A, Foster AP, Kwochka KW, editors. Advances in Veterinary Dermatology, 5. Oxford, UK: Blackwell Publishing; 2005. p. 10-6.
    13. Pinheiro D, Singh Y, Grant CR, Appleton RC, Sacchini F, Walker KR, Chadbourne AH, Palmer CA, Armitage-Chan E, Thompson I, Williamson L. Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog. Immunology. 2011 Jan;132(1):111-22. Go to original source... Go to PubMed...
    14. Takahata Y, Nomura A, Takada H, Ohga S, Furuno K, Hikino S, Nakayama H, Sakaguchi S, Hara T. CD25+ CD4+ Tcells in human cord blood: An immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene. Exp Hematol. 2004 Jul;32(7):622-9. Go to original source... Go to PubMed...

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