Vet Med - Czech, 2022, 67(1):38-45 | DOI: 10.17221/85/2020-VETMED

Autologous adipose-derived mesenchymal stem cells and hydroxyapatite for bone defect in rabbitsOriginal Paper

GG Franco¹, BW Minto¹, LP Coelho¹, PF Malard², ER Carvalho¹, FYK Kawamoto¹, BM Alcantara¹, LGGG Dias¹

¹ Department of Clinical and Veterinary Surgery, Faculty of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP - Brazil

² BioCell, Brasília, DF - Brazil

This study aims to evaluate the effect of autologous adipose-derived mesenchymal stem cells (AAD-MSC), with and without synthetic absorbable hydroxyapatite (HAP-91), on the bone regeneration in rabbits. Thirty-four female white New Zealand rabbits were submitted to a 10 mm distal diaphyseal radius ostectomy, divided into 3 experimental groups according to the treatment established. The bone gap was filled with 0.15 ml of a 0.9% saline solution containing two million AAD-MSC (G1), or AAD-MSC associated with HAP-91 (G2). The control group (CG) received only 0.15 ml of the 0.9% saline solution. Radiographs were made post-operatively, and after 15, 30, 45 and 90 days. Fifty percent of the samples were submitted to a histological examination at 45 days and the remaining ones at 90 days post-operatively. Radiographically, the periosteal reaction, bone callus volume and bone bridge quality were superior in G2 (P < 0.05). Histologically, the bone repair was faster and more efficient in G1 at 45 days (P < 0.05). In conclusion, AAD-MSC improved the regeneration on the experimentally induced bone defects in rabbits; however, the use of hydroxyapatite requires caution given the granulomatous reaction produced in the species.

Keywords: adipose derived MSC; cell transplantation; fracture healing; Oryctolagus cuniculus

Received: April 9, 2020; Accepted: August 2, 2021; Published: January 15, 2022  Show citation