Vet Med - Czech, 2022, 67(2):87-98 | DOI: 10.17221/141/2020-VETMED

Comparative analysis of the exosomal contents of DF-1 cells infected by ALV-JOriginal Paper

J Yang, P Zhuang, Z Cheng, G Wang ORCID...
Department of Fundamental Veterinary, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China

Exploration of the abnormal expression of exosomal molecules during the infection of avian leukosis virus subgroup J (ALV-J) is essential to provide a deeper understanding of the exosome's role in the viral pathogenesis involved. The study aimed to investigate the differentially expressed proteins and miRNAs of the exosomes derived from DF-1 cells infected by ALV-J, their gene function and involved signal pathways. We isolated exosomes from DF-1 cells infected by ALV-J. The differentially expressed proteins and miRNAs of the exosomes were determined by proteomics and transcription detection technology. A Gene Ontology (GO) analysis and a Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway analysis identified the miRNAs target genes and the signal pathways regulated by the different proteins or/and miRNAs. A total of 116 proteins (58 upregulated and 58 downregulated) and 3 miRNAs (all upregulated) were determined. These proteins were involved in 155 signal pathways, in which the highest number of proteins involved in the cancer pathway was (up to) seven. The target genes of the miRNAs were involved in 3 signal pathways. Both the proteins and target genes of the miRNAs were involved in the Ribosome pathway and ECM-receptor interaction pathway. The results suggested that the ALV-J infection changed the proteins and miRNAs of the exosomes significantly.

Keywords: avian leukosis virus subgroup J (ALV-J); exosomes; proteomics; transcriptomics

Received: July 4, 2020; Accepted: September 16, 2021; Published: February 15, 2022  Show citation

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Yang J, Zhuang P, Cheng Z, Wang G. Comparative analysis of the exosomal contents of DF-1 cells infected by ALV-J. Vet Med - Czech. 2022;67(2):87-98. doi: 10.17221/141/2020-VETMED.
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    References

    1. Anders S, Huber W. Differential expression analysis for sequence count data. Genome Biol. 2010;11(10):R106. Go to original source... Go to PubMed...
    2. Bao Y, Wang L, Shi L, Yun F, Liu X, Chen Y, Chen C, Ren Y, Jia Y. Transcriptome profiling revealed multiple genes and ECM-receptor interaction pathways that may be associated with breast cancer. Cell Mol Biol Lett. 2019 Jun 6;24(6):38-58. Go to original source... Go to PubMed...
    3. Bhaskaran M, Mohan M. MicroRNAs: History, biogenesis, and their evolving role in animal development and disease. Vet Pathol. 2014 Jul;51(4):759-74. Go to original source... Go to PubMed...
    4. Bustelo XR, Dosil M. Ribosome biogenesis and cancer: Basic and translational challenges. Curr Opin Genet Dev. 2018 Feb;48(2):22-9. Go to original source... Go to PubMed...
    5. Cao L, Chen Y, Zhang M, Xu DQ, Liu Y, Liu T, Liu SX, Wang P. Identification of hub genes and potential molecular mechanisms in gastric cancer by integrated bioinformatics analysis. PeerJ. 2018 Jul 2;6:e5180. Go to original source... Go to PubMed...
    6. Catez F, Dalla Venezia N, Marcel V, Zorbas C, Lafontaine DLJ, Diaz JJ. Ribosome biogenesis: An emerging druggable pathway for cancer therapeutics. Biochem Pharmacol. 2019 Jan;159(1):74-81. Go to original source... Go to PubMed...
    7. Chuammitri P, Vannamahaxay S, Sornpet B, Pringproa K, Patchanee P. Detection and characterization of microRNA expression profiling and its target genes in response to canine parvovirus in Crandell Reese feline kidney cells. PeerJ. 2020 Feb 12;8(2):e8522. Go to original source... Go to PubMed...
    8. Dong X, Zhao P, Chang S, Ju S, Li Y, Meng F, Sun P, Cui Z. Synergistic pathogenic effects of co-infection of subgroup J avian leukosis virus and reticuloendotheliosis virus in broiler chickens. Avian Pathol. 2015a;44(1):43-9. Go to original source... Go to PubMed...
    9. Dong X, Zhao P, Li W, Chang S, Li J, Li Y, Ju S, Sun P, Meng F, Liu J, Cui Z. Diagnosis and sequence analysis of avian leukosis virus subgroup J isolated from Chinese Partridge Shank chickens. Poult Sci. 2015b Apr;94(4):668-72. Go to original source... Go to PubMed...
    10. Friedlander MR, Mackowiak SD, Li N, Chen W, Rajewsky N. miRDeep2 accurately identifies known and hundreds of novel microRNA genes in seven animal clades. Nucleic Acids Res. 2012 Jan;40(1):37-52. Go to original source... Go to PubMed...
    11. Hartig SM, Hamilton MP, Bader DA, McGuire SE. The miRNA interactome in metabolic homeostasis. Trends Endocrinol Metab. 2015 Dec;26(12):733-45. Go to original source... Go to PubMed...
    12. Khan MB, Lang MJ, Huang MB, Raymond A, Bond VC, Shiramizu B, Powell MD. Nef exosomes isolated from the plasma of individuals with HIV-associated dementia (HAD) can induce Aβ(1-42) secretion in SH-SY5Y neural cells. J Neurovirol. 2016 Apr;22(2):179-90. Go to original source... Go to PubMed...
    13. Khatri B, Seo D, Shouse S, Pan JH, Hudson NJ, Kim JK, Bottje W, Kong BC. MicroRNA profiling associated with muscle growth in modern broilers compared to an unselected chicken breed. BMC Genomics. 2018 Sep 17;19(1): 683-93. Go to original source... Go to PubMed...
    14. Kim C, Yang H, Park I, Chon HJ, Kim JH, Kwon WS, Lee WS, Kim TS, Rha SY. Rho GTPase RhoJ is associated with gastric cancer progression and metastasis. J Cancer. 2016 Jul 8;7(11):1550-6. Go to original source... Go to PubMed...
    15. Kinoshita T, Nohata N, Watanabe-Takano H, Yoshino H, Hidaka H, Fujimura L, Fuse M, Yamasaki T, Enokida H, Nakagawa M, Hanazawa T, Okamoto Y, Seki N. Actinrelated protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma. Int J Oncol. 2012 Jun; 40(6):1770-8.
    16. Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10(3):R25. Go to original source... Go to PubMed...
    17. Li T, Gao X, Han L, Yu J, Li H. Identification of hub genes with prognostic values in gastric cancer by bioinformatics analysis. World J Surg Oncol. 2018 Jun 19;16(1):114-26. Go to original source... Go to PubMed...
    18. Marimpietri D, Petretto A, Raffaghello L, Pezzolo A, Gagliani C, Tacchetti C, Mauri P, Melioli G, Pistoia V. Proteome profiling of neuroblastoma-derived exosomes reveal the expression of proteins potentially involved in tumor progression. PLoS One. 2013 Sep 19;8(9):e75054. Go to original source... Go to PubMed...
    19. Momen-Heravi F, Bala S, Bukong T, Szabo G. Exosomemediated delivery of functionally active miRNA-155 inhibitor to macrophages. Nanomedicine. 2014 Oct;10(7): 1517-27. Go to original source... Go to PubMed...
    20. Montecalvo A, Larregina AT, Shufesky WJ, Stolz DB, Sullivan ML, Karlsson JM, Baty CJ, Gibson GA, Erdos G, Wang Z, Milosevic J, Tkacheva OA, Divito SJ, Jordan R, Lyons-Weiler J, Watkins SC, Morelli AE. Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes. Blood. 2012 Jan 19;119(3): 756-66. Go to original source... Go to PubMed...
    21. Payne LN, Brown SR, Bumstead N, Howes K, Frazier JA, Thouless ME. A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol. 1991 Apr;72(Pt 4): 801-7. Go to original source... Go to PubMed...
    22. Poveda E, Freeman ML. Hot news: Exosomes as new players in HIV pathogenesis - New data from the IAS 2017. AIDS Rev. 2017 Oct-Dec;19(3):173-5. Go to original source...
    23. Simpson RJ, Kalra H, Mathivanan S. ExoCarta as a resource for exosomal research. J Extracell Vesicles. 2012 Apr 16;1 (4):18374-80. Go to original source... Go to PubMed...
    24. Wang G, Wang Z, Zhuang P, Zhao X, Cheng Z. Exosomes carring gag/env of ALV-J possess negative effect on immunocytes. Microb Pathog. 2017 Nov;112(11):142-7. Go to original source... Go to PubMed...
    25. Yang X, Gao W, Liu H, Li J, Chen D, Yuan F, Zhang Z, Wang H. MicroRNA transcriptome analysis in chicken kidneys in response to differing virulent infectious bronchitis virus infections. Arch Virol. 2017 Nov;162(11): 3397-405. Go to original source... Go to PubMed...
    26. Yu Z, Gao X, Liu C, Lv X, Zheng S. Analysis of microRNA expression profile in specific pathogen-free chickens in response to reticuloendotheliosis virus infection. Appl Microbiol Biotechnol. 2017 Apr;101(7):2767-77. Go to original source... Go to PubMed...
    27. Zhang J, Li S, Li L, Li M, Guo C, Yao J, Mi S. Exosome and exosomal microRNA: Trafficking, sorting, and function. Genomics Proteomics Bioinformatics. 2015 Feb;13(1): 17-24. Go to original source... Go to PubMed...
    28. Zhou D, Xue J, He S, Du X, Zhou J, Li C, Huang L, Nair V, Yao Y, Cheng Z. Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs. Retrovirology. 2018 Jul 3;15(1):45-56. Go to original source... Go to PubMed...
    29. Zhou Y, Tian W, Zhang M, Ren T, Sun G, Jiang R, Han R, Kang X, Yan F. Transcriptom analysis revealed regulation of dexamethasone induced microRNAs in chicken thymus. J Cell Biochem. 2019 Apr;120(4):6570-9. Go to original source... Go to PubMed...
    30. Zhou R, Rana TM. RNA-based mechanisms regulating hostvirus interactions. Immunol Rev. 2013 May;253(1):97-111. Go to original source... Go to PubMed...

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