Vet Med - Czech, 2023, 68(1):27-32 | DOI: 10.17221/57/2022-VETMED

Antimicrobial activities of nano-emulsion of virgin coconut oilOriginal Paper

DC Widianingrum1, H Khasanah1, L Purnamasari1, ME Krismaputri1, SG Hwang2
1 Department of Animal Science, Faculty of Agriculture, University of Jember, Jember, Indonesia
2 Department of Animal Life and Environmental Science, Hankyong National University, Anseong-si, Gyeonggi-do, Republic of Korea

This study aimed to determine the nano-emulsion of virgin coconut oil (n-VCO) formula that can produce the best size and zone inhibition of antimicrobial activity. The VCO was formulated with the different percentages of Tween 80 (P1: 24%, P2: 25%, P3: 26%) and sorbitol (P1: 36%, P2: 35%, P3: 34%). The particle size of the n-VCO emulsion was observed under transmission electron microscopy (TEM). The antimicrobial activity test of the n-VCO was determined by a challenge test using Salmonella Typhi (S. Typhi), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli) bacteria. The data were analysed by a one-way ANOVA (P < 0.05). The significant data were furthermore tested by Duncan’s multiple ranges (SPSS v26.0). This study showed that the P3 formulation (26% Tween 80 and 34% sorbitol) produced the best n-VCO among all the treatments showing a particle size of 5–100 nm. Formulas P1 and P2 produced particle sizes of about 500–1 000 nm. The antimicrobial test showed that the P3 formula had a strong inhibitory effect on S. Typhi (7.442 ± 0.52 mm), S. aureus (8.380 ± 0.49 mm), and E. coli (6.490 ± 0.82 mm). This study concluded that the formula of the detergent strongly influences the particle size of the n-VCO. The n-VCO has enormous potential to be used as an alternative antimicrobial.

Keywords: alternative antimicrobial; nano-emulsification formula; natural resources

Received: June 22, 2022; Accepted: December 15, 2022; Prepublished online: January 18, 2023; Published: January 19, 2023  Show citation

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Widianingrum D, Khasanah H, Purnamasari L, Krismaputri M, Hwang S. Antimicrobial activities of nano-emulsion of virgin coconut oil. Vet Med - Czech. 2023;68(1):27-32. doi: 10.17221/57/2022-VETMED.
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    References

    1. Abbas AA, Assikong EB, Akeh M, Upla P, Tuluma T. Antimicrobial activity of coconut oil and its derivative (lauric acid) on some selected clinical isolates. Int J Med Sci Clin Invent. 2017 Aug;4(8):3173-7. Go to original source...
    2. Albuquerque J, Casal S, Pascoa RNMJ, Van Dorpe I, Fonseca AJM, Cabrita ARJ, Neves AR, Reis S. Applying nanotechnology to increase the rumen protection of amino acids in dairy cows. Sci Rep. 2020 Apr 22;10(1):1-12. Go to original source... Go to PubMed...
    3. Anwer MK, Jamil S, Ibnouf EO, Shakeel F. Enhanced antibacterial effects of clove essential oil by nanoemulsion. J Oleo Sci. 2014;63(4):347-54. Go to original source... Go to PubMed...
    4. Aulia Y. Pengaruh variasi konsentrasi Tween 80 dan Sorbitol terhadap aktivitas antioksidan minyak alpukat (avocado oil) dalam formulasi nanoemulsi [The effect of variations in the concentration of Tween 80 and sorbitol on the antioxidant activity of avocado oil in nanoemulsion formulations] [bachelor thesis]. Medan, Indonesia: University of Sumatera Utara; 2017. 1294 p. Indonesian.
    5. Bouchemal K, Briancon S, Perrier E, Fessi H. Nano-emulsion formulation using spontaneous emulsification: Solvent, oil and surfactant optimisation. Int J Pharm. 2004 Aug 6;280(1-2):241-51. Go to original source... Go to PubMed...
    6. Carandang EV. Health benefits of virgin coconut oil. Indian Coconut J Cochin. 2008 Jan;38(9): [8].
    7. Dayrit FM. Lauric acid is a medium-chain fatty acid, coconut oil is a medium-chain triglyceride. Philipp J Sci. 2014 Dec;143(2):157-66.
    8. Dayrit FM. The properties of lauric acid and their significance in coconut oil. J Am Oil Chem Soc. 2015;92(1):1-15. Go to original source...
    9. Dhineshkumar V, Ramasamy D, Sudha K. Nanotechnology application in food and dairy processing. Int J Farm Sci. 2015;5(3):274-88.
    10. Enig M. Coconut: In support of good health in the 21st century [Internet]. 2019 [cited 2022 Jan 1]. Avaliable from: http://coconutoil.com/coconut_oil_21st_century.
    11. Fakhrurrazi, Hakim RF, Keumala CN. Pengaruh daun asam jawa (Tamarindus indica Linn) terhadap pertumbuhan Candida Albicans [The effect of java acid leaves (Tamarindus Indica Linn) on the growth of Candida albicans]. J Syiah Kuala Dent Soc. 2016;1(1):29-34. Indonesian.
    12. Hakim NA, Arianto A, Bangun H. Formulasi dan evaluasi nanoemulsi dari extra virgin olive oil (minyak saitun ekstra murni) sebagai anti-aging [Formulation and evaluation of nanoemulsions of extra virgin olive oil as anti-aging]. TM Conf Series Nano Pharm. 2017;1(2):397-403. Indonesian. Go to original source...
    13. Isyanti M, Sirait SD. Fraksinasi asam laurat, short chain triglyceride (SCT) dan medium chain tiglyceride (MCT) daro minyak kelapa murni [Fractionation of lauric acid, short chain fatty trygliceride (MCT) and medium chain triglyceride (MCT) from virgin coconut oil]. Warta IHP/J Agro-Based Industry. 2021;38(2):160-8. Indonesian. Go to original source...
    14. Khatri P, Shao J. Mechanism and structural factors of lipid and surfactant in the formation of self-emulsified nanoemulsion. J Pharm Sci. 2018 Aug;107(8):2198-207. Go to original source... Go to PubMed...
    15. Koroleva M, Nagovitsina T, Yurtov E. Nanoemulsions stabilized by non-ionic surfactants: Stability and degradation mechanisms. Phys Chem Chem Phys. 2018 Apr 18;20(15):10369-77. Go to original source... Go to PubMed...
    16. Marhamati M, Ranjbar G, Rezaie M. Effects of emulsifiers on the physicochemical stability of oil-in-water nanoemulsions: A critical review. J Mol Liq. 2021 Oct;340(1):117218. Go to original source...
    17. Md Saari NH, Chua LS, Hasham R, Yuliati L. Curcumin-loaded nanoemulsion for better cellular permeation. Sci Pharm. 2020 Oct;88(4):44. Go to original source...
    18. Mulia K, Putri GA, Krisanti E. Encapsulation of mangosteen extract in virgin coconut oil based nanoemulsions: Preparation and characterization for topical formulation. Mater Sci Forum. 2018 Oct;929:234-42. Go to original source...
    19. Nasir NAMM, Abllah Z, Azura Jalaludin A, Azura Shahdan I, Hayati Wan Abd Manan WN. Virgin coconut oil and its antimicrobial properties against pathogenic microorganisms: A review. In: Proceedings of the International Dental Conference of Sumatera Utara 2017 (IDCSU 2017); Paris, France; Atlantis Press; 2018. p. 192-9. Go to original source...
    20. Pan X, Chen F, Wu T, Tang H, Zhao Z. The acid, bile tolerance and antimicrobial property of Lactobaccilus aci-dophillus NIT. Food Control. 2009;20(6):598-602. Go to original source...
    21. Rao J, McClements DJ. Lemon oil solubilization in mixed surfactant solutions: Rationalizing microemulsion & nanoemulsion formation. Food Hydrocoll. 2012 Jan;26(1):268-76. Go to original source...
    22. Samson S, Basri M, Fard Masoumi HR, Abdul Malek E, Abedi Karjiban R. An artificial neural network based analysis of factors controlling particle size in a virgin coconut oil-based nanoemulsion system containing copper peptide. PLoS One. 2016 Jul 6;11(7):e0157737. Go to original source... Go to PubMed...
    23. Suraweera RK, Pasansi HGP, Sakeena MHF. Assessing the characterizations of ketoprofen loaded and unloaded virgin coconut oil based creamy nanoemulsion. Asian J Pharm Clin Res. 2015;8(1):275-9.
    24. Tangwatcharin P, Khopaibool P. Activity of virgin coconut oil, lauric acid or monolaurin in combination with lactic acid against Staphylococcus aureus. Southeast Asian J Trop Med Public Health. 2012 Jul;43(4):969-85.
    25. Tatli Seven P, Seven I, Gul Baykalir B, Mutlu SI, Salem AZ. Nanotechnology and nano-propolis in animal production and health: An overview. Ital J Anim Sci. 2018;17(4):921-30. Go to original source...
    26. Wesley SJ, Raja P, Raj AA, Tiroutchelvamae D. Review on-nanotechnology applications in food packaging and safety. Int J Eng Res. 2014 Nov;3(11):645-51. Go to original source...
    27. Widianingrum DC, Salasia SIO, Noviandi CT. Kecernaan dan karakteristik fermentasi rumen in vitro ransum ruminansia dengan suplementasi virgin coconut oil terproteksi [In vitro digestibility and rumen fermentation characteristic of ruminants ration supplemented with protected virgin coconut oil]. In: Proceeding National Seminar on Animal Husbandry and Veterinary Technology; Jember, Indonesia; Oct 15th-17th; 2019a. p. 168-76. Indonesian.
    28. Widianingrum DC, Noviandi CT, Salasia SIO. Antibacterial and immunomodulator activities of virgin coconut oil (VCO) against Staphylococcus aureus. Heliyon. 2019b Oct 20;5(10):e02612. Go to original source... Go to PubMed...
    29. Widianingrum DC, Salasia SIO. Immunomodulatory effects of virgin coconut oil in Wistar rats infected with Staphylococcus aureus. JITV. 2021;26(1):31-8. Go to original source...
    30. Yildirim ST, Oztop MH, Soyer Y. Cinnamon oil nanoemulsions by spontaneous emulsification: Formulation, characterization and antimicrobial activity. Lebenson Wiss Technol. 2017;84:122-8. Go to original source...
    31. Zentek J, Ferrara F, Pieper R, Tedin L, Meyer W, Vahjen W. Effects of dietary combinations of organic acids and medium chain fatty acids on the gastrointestinal microbial ecology and bacterial metabolites in the digestive tract of weaning piglets. J Anim Sci. 2013 Jul;91(7):3200-10. Go to original source... Go to PubMed...

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