Vet Med - Czech, 2023, 68(2):45-56 | DOI: 10.17221/48/2022-VETMED

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A reviewReview

S Kumar1, MS Chaves1, AFB da Silva2, WG Vale2, STR Filho3, JC Ferreira-Silva4, LM Melo5, VJF Freita1
1 Laboratory of Physiology and Control of Reproduction, State University of Ceará, Fortaleza, Brazil
2 Postgraduate Program in Veterinary Science, State University of Ceará, Fortaleza, Brazil
3 Animal Reproduction Sector, Federal Rural University of the Amazon, Belém, Brazil
4 Animal Reproduction Sector, Federal Rural University of Pernambuco, Recife, Brazil
5 Molecular Genetics Research Unit, University Center Fametro, Fortaleza, Brazil

Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes.

Keywords: calving rate; embryo transfer; IVEP; oocytes; OPU; pregnancy rate

Received: May 16, 2020; Accepted: December 15, 2022; Prepublished online: February 21, 2023; Published: February 24, 2023  Show citation

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Kumar S, Chaves M, da Silva A, Vale W, Filho S, Ferreira-Silva J, et al.. Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review. Vet Med - Czech. 2023;68(2):45-56. doi: 10.17221/48/2022-VETMED.
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