Vet Med - Czech, 2023, 68(9):359-367 | DOI: 10.17221/42/2023-VETMED

Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genesOriginal Paper

V Ozavci ORCID...1, HT Yuksel Dolgun ORCID...2, S Kirkan ORCID...2, Y Seferoglu ORCID...2, Z Semen ORCID...3, U Parin ORCID...2
1 Department of Microbiology, Faculty of Veterinary Medicine, Dokuz Eylul University, Izmir, Turkiye
2 Department of Microbiology, Faculty of Veterinary Medicine, Aydin Adnan Menderes University, Aydin, Turkiye
3 Department of Biochemistry, Faculty of Veterinary Medicine, Dokuz Eylul University, Izmir, Turkiye

Streptococcus (S.) species are important pathogens that cause mastitis in sheep. The study aimed to examine Streptococcus species in sheep milk with subclinical mastitis, assessing their prevalence, antimicrobial resistance, and virulence genes. A total of 200 milk samples were collected from sheep farms in İzmir’s five districts. Out of 32 (28.6%) Streptococcus isolates identified by phenotypic methods, 25 were genotypically identified as S. uberis, 5 as S. agalactiae, and 2 as S. dysgalactiae. Disk diffusion was used to determine the antimicrobial resistance of the isolates. PCR was employed to identify antimicrobial resistance and virulence genes in the isolates. The highest resistance was found for cloxacillin (100%), and the highest sensitivity was found for florfenicol (84%). The most common resistance gene combination was tetM+tetS (3/32) for S. uberis in 9.4%. A total of five virulence genes were detected. GapC+sua (56.2%) constituted the most common gene pattern. The highest virulence gene gapC was detected in 78.1% (25/32) of the isolates. The cylE gene was not detected (0%) in the isolates. Streptococcus species may play a role in mastitis in sheep, emphasising the need for meticulous hygienic milking practices.

Keywords: dairy sheep; mastitis; PCR; Streptococcus strains

Received: April 10, 2023; Revised: September 5, 2023; Accepted: September 12, 2023; Prepublished online: September 25, 2023; Published: September 29, 2023  Show citation

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Ozavci V, Yuksel Dolgun H, Kirkan S, Seferoglu Y, Semen Z, Parin U. Evaluation of Streptococcus species isolated from subclinical sheep mastitis by molecular methods and determination of virulence factors and antimicrobial resistance genes. Vet Med - Czech. 2023;68(9):359-367. doi: 10.17221/42/2023-VETMED.
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