Vet Med - Czech, 2024, 69(3):84-93 | DOI: 10.17221/111/2023-VETMED

Effect of vitamin D3 and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acidOriginal Paper

A Gezer ORCID...1, S Yediel Aras ORCID...2, N Kilic Baygutalp ORCID...3, E Karadag Sari ORCID...4, G Bedir ORCID...5, B Mokhtare6, K Yilmaz ORCID...4
1 Vocational School of Health Services, Ataturk University, Erzurum, Turkiye
2 Department of Midwifery, Faculty of Health Sciences, Kafkas University, Kars, Turkiye
3 Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkiye
4 Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkiye
5 Department of Histology and Embryology, Ataturk University School of Medicine, Erzurum, Turkiye
6 Department of Pathology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkiye

In this study, the effects of vitamin D3 (Vit. D) and a stinging nettle (Urtica dioica L. (UD)) extract were examined using histopathological and immunohistochemical methods in the stomach tissues of an experimentally created rat model of CD. The CD model was created using trinitrobenzene sulfonic acid (TNBS). The animals in the study were divided into control, TNBS, TNBS+Vit. D, and TNBS+UD groups. At the end of the experiment, the animals were euthanised and their stomach tissues were evaluated for necrosis, degeneration, apoptosis, and inflammation. Additionally, an immunohistochemical method was applied to determine the somatostatin (SSTR), aquaporin-1 (AQP-1), caspase-3, and tumour necrosis factor-alpha (TNF-α) immunoreactivity in the gastric tissues. In the evaluations, degenerative and necrotic changes and mononuclear cell infiltration areas were observed in the TNBS group, but such changes could be improved with Vit. D and UD applications. The results suggest that the combination of the Vit. D and UD extract may have a protective and therapeutic role in mitigating TNBS-induced damage to the gastric tissues, potentially through the regulation of SSTR, AQP-1, caspase-3, and TNF-α expression. This indicates a promising avenue for further research and the exploration of these compounds in the context of gastrointestinal health.

Keywords: aquaporin-1; Crohn’s disease; somatostatin; TNF-α

Received: October 31, 2023; Revised: January 24, 2024; Accepted: February 23, 2024; Published: March 28, 2024  Show citation

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Gezer A, Yediel Aras S, Kilic Baygutalp N, Karadag Sari E, Bedir G, Mokhtare B, Yilmaz K. Effect of vitamin D3 and a stinging nettle extract on the gastric tissue of rats administered with trinitrobenzene sulfonic acid. Vet Med - Czech. 2024;69(3):84-93. doi: 10.17221/111/2023-VETMED.
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