Vet Med - Czech, 2024, 69(4):115-122 | DOI: 10.17221/114/2023-VETMED

Antibacterial effect of curcumin on Salmonella Typhimurium: In vitro and food model studiesOriginal Paper

G Terzi Gulel, S Kanat ORCID..., E Kucukgoz ORCID...
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkiye

Salmonellosis is a major foodborne disease transmitted from contaminated poultry products worldwide. Although a wide variety of chemical agents are used in the prevention of foodborne Salmonella spp. infections, consumers prefer natural additives, that do not harm human health and do not impair the characteristics of food. Curcumin is a yellow-coloured, hydrophobic polyphenol obtained from the rhizome of the Curcuma longa L. plant known as turmeric. The purpose of this study was to evaluate curcumin’s antibacterial activity against S. Typhimurium in chicken meat and in vitro. In the first step, chicken samples were experimentally contaminated with S. Typhimurium at a level of 2.8 × 10–7 CFU/ml. Then, they were kept in a 1, 2, and 3% curcumin solution for 15 minutes. At the end of the treatment, chicken samples were stored at +4 °C. The number of S. Typhimurium in chicken samples was determined according to EN ISO 6579-1. In the result of the study, the number of S. Typhimurium decreased by 2.37, 2.71, and 2.84 log levels at the end of the 6th day as a result of the 1, 2 and 3% curcumin treatment, respectively. The MIC value of curcumin was determined to be 362 µg/ml for S. Typhimurium.

Keywords: chicken; curcumin; S. Typhimurium

Received: November 4, 2023; Revised: March 14, 2024; Accepted: March 26, 2024; Published: April 26, 2024  Show citation

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Terzi Gulel G, Kanat S, Kucukgoz E. Antibacterial effect of curcumin on Salmonella Typhimurium: In vitro and food model studies. Vet Med - Czech. 2024;69(4):115-122. doi: 10.17221/114/2023-VETMED.
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    References

    1. Adamczak A, Ozarowski M, Karpinski TM. Curcumin, a natural antimicrobial agent with strain-specific activity. Pharmaceuticals (Basel). 2020 Jul 16;13(7):153. Go to original source... Go to PubMed...
    2. Altunatmaz SS, Aksu FY, Issa G, Kahraman BB, Altiner DD, Buyukunal S. Antimicrobial effects of curcumin against L. monocytogenes, S. aureus, S. Typhimurium and E. coli O157: H7 pathogens in minced meat. Vet Med-Czech. 2016 May;61(5):256-62. Go to original source...
    3. Alves TM, Silva AF, Brandao M, Grandi TS, Smania E, Smania Junior A, Zani CL. Biological screening of Brazilian medicinal plants. Mem Inst Oswaldo Cruz. 2000 May-Jun;95(3):367-73. Go to original source... Go to PubMed...
    4. CDC - Centers for Disease Control and Prevention. Salmonella [Internet]. 2023 [cit 2023 Jul 15]. Available from: www.cdc.gov/salmonella/index.html.
    5. CLSI - Clinical and Laboratory Standards Institute. Performance standards for anti-microbial susceptibility testing. CLSI Supplement M100, 30th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2020.
    6. Dai C, Lin J, Li H, Shen Z, Wang Y, Velkov T, Shen J. The natural product curcumin as an antibacterial agent: Current achievements and problems. Antioxidants (Basel). 2022 Feb 25;11(3):459. Go to original source... Go to PubMed...
    7. Dar M, Ahmad S, Bhat S, Ahmed R, Urwat U, Mumtaz P, Bhat SA, Dar TA, Shah RA, Ganai N. Salmonella Typhimurium in poultry: A review. Worlds Poult Sci J. 2017 Sep;73(2):345-54. Go to original source...
    8. EC - European Commission. Commission Regulation (EC) No. 2073/2005 of the European Parliament and of the Council of 15 November 2005 on microbiological criteria for foodstuffs. OJEU. L338:1-26. 2005.
    9. EFSA - European Food Safety Authority. Refined exposure assessment for curcumin (E 100). EFSA J. 2014 Oct;12(10):1-43. Go to original source...
    10. Ehuwa O, Jaiswal AK, Jaiswal S. Salmonella, food safety and food handling practices. Foods. 2021 Apr 21;10(5):907. Go to original source... Go to PubMed...
    11. Gao J, Matthews KR. Effects of the photosensitizer curcumin in inactivating foodborne pathogens on chicken skin. Food Control. 2020 Mar;109:1-7. Go to original source...
    12. Gao J, Srichamnong W, Chathiran W, Matthews KR. Influences of photosensitizer curcumin on microbial survival and physicochemical properties of chicken during storage. Poult Sci. 2023 Feb;102(2):102417. Go to original source... Go to PubMed...
    13. Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as "Curecumin": From kitchen to clinic. Biochem Pharmacol. 2008 Feb 15;75(4):787-809. Go to original source... Go to PubMed...
    14. Govaris A, Solomakos N, Pexara A, Chatzopoulou PS. The antimicrobial effect of oregano essential oil, nisin and their combination against Salmonella Enteritidis in minced sheep meat during refrigerated storage. Int J Food Microbiol. 2010 Feb 28;137(2-3):175-80. Go to original source... Go to PubMed...
    15. Gul P, Bakht J. Antimicrobial activity of turmeric extract and its potential use in food industry. J Food Sci Technol. 2015 Apr;52(4):2272-9. Go to original source... Go to PubMed...
    16. Gunes H, Gulen D, Mutlu R, Gumus A, Tas T, Topkaya AE. Antibacterial effects of curcumin: An in vitro minimum inhibitory concentration study. Toxicol Ind Health. 2016 Feb;32(2):246-50. Go to original source... Go to PubMed...
    17. Gupta A, Mahajan S, Sharma R. Evaluation of antimicrobial activity of Curcuma longa rhizome extract against Staphylococcus aureus. Biotechnol Rep (Amst). 2015 Feb 18;6:51-5. Go to original source... Go to PubMed...
    18. Islam MR, Ahamed R, Rahman MO, Akbar MA, Al-Amin M, Alam KD, Lyzu F. In vitro antimicrobial activities of four medicinally important plants in Bangladesh. Eur J Sci Res. 2010;39(2):199-206.
    19. ISO - International Organization for Standardization. EN ISO 6579-1:2017 Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella. Part 1. Detection of Salmonella spp. 2017. 50 p.
    20. Jajere SM. A review of Salmonella enterica with particular focus on the pathogenicity and virulence factors, host specificity and antimicrobial resistance including multidrug resistance. Vet World. 2019;12(4):504-21. Go to original source... Go to PubMed...
    21. Keyvan E, Tutun H, Kahraman HA, Erdi S, Demirtas A, Donmez S, Akyuz AO. Determination of time dependent antibacterial activities of curcumin, carvacrol and styrax liquidus on Salmonella Enteritidis. Ankara Univ Vet Fak Derg. 2022 Sep;69(4):355-60. Go to original source...
    22. Kilic S, Oz E, Oz F. Effect of turmeric on the reduction of heterocyclic aromatic amines and quality of chicken meatballs. Food Control. 2021 Oct;128:1-7. Go to original source...
    23. Lan X, Liu Y, Wang L, Wang H, Hu Z, Dong H, Yu Z, Yuan Y. A review of curcumin in food preservation: Delivery system and photosensitization. Food Chem. 2023 Oct 30;424:136464. Go to original source... Go to PubMed...
    24. Lawhavinit O, Kongkathip N, Kongkathip B. Antimicrobial activity of curcuminoids from Curcuma longa L. on pathogenic bacteria of shrimp and chicken. Kasetsart J (Nat Sci). 2010 May-Jun;44(3):364-71.
    25. Marathe SA, Balakrishnan A, Negi VD, Sakorey D, Chandra N, Chakravortty D. Curcumin reduces the motility of Salmonella enterica serovar Typhimurium by binding to the flagella, thereby leading to flagellar fragility and shedding. J Bacteriol. 2016 Jun 13;198(13):1798-811. Go to original source... Go to PubMed...
    26. Nelson KM, Dahlin JL, Bisson J, Graham J, Pauli GF, Walters MA. The essential medicinal chemistry of curcumin: Miniperspective. J Med Chem. 2017 Jan;60(5):1620-37. Go to original source... Go to PubMed...
    27. Prasad S, Gupta SC, Tyagi AK, Aggarwal BB. Curcumin, a component of golden spice: From bedside to bench and back. Biotechnol Adv. 2014 Nov 1;32(6):1053-64. Go to original source... Go to PubMed...
    28. Priyadarsini KI, Maity DK, Naik GH, Kumar MS, Unnikrishnan MK, Satav JG, Mohan H. Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin. Free Radic Biol Med. 2003 Sep 1;35(5):475-84. Go to original source... Go to PubMed...
    29. Punchihewage-Don AJ, Hawkins J, Adnan AM, Hashem F, Parveen S. The outbreaks and prevalence of antimicrobial resistant Salmonella in poultry in the United States: An overview. Heliyon. 2022 Nov 12;8(11):e11571. Go to original source... Go to PubMed...
    30. Sakha H, Hora R, Shrestha S, Acharya S, Dhakal D, Thapaliya S, Prajapati K. Antimicrobial activity of ethanolic extract of medicinal plants against human pathogenic bacteria. TUJM. 2018;5(1):1-6. Go to original source...
    31. Sharifi-Rad J, Rayess YE, Rizk AA, Sadaka C, Zgheib R, Zam W, Sestito S, Rapposelli S, Neffe-Skocinska K, Zielinska D, Salehi B, Setzer WN, Dosoky NS, Taheri Y, El Beyrouthy M, Martorell M, Ostrander EA, Suleria HAR, Cho WC, Maroyi A, Martins N. Turmeric and its major compound curcumin on health: Bioactive effects and safety profiles for food, pharmaceutical, biotechnological and medicinal applications. Front Pharmacol. 2020 Sep 15;11:01021. Go to original source... Go to PubMed...
    32. Sujarwanta RO, Jamhari J, Suryanto E, Yuliatmo R, Prayitno AH. Physicochemical and sensory characteristics of chicken nugget with curcuma (Curcuma zanthorrhiza) flour fortification. IOP Conf Ser: Earth Environ Sci. 2019;387:1-5. Go to original source...
    33. Teow SY, Liew K, Ali SA, Khoo AS, Peh SC. Antibacterial action of curcumin against Staphylococcus aureus: A brief review. J Trop Med. 2016;2016:2853045. Go to original source... Go to PubMed...
    34. Tripathy S, Verma DK, Thakur M, Patel AR, Srivastav PP, Singh S, Gupta AK, Chavez-Gonzalez ML, Aguilar CN, Chakravorty N, Verma HK, Utama GL. Curcumin extraction, isolation, quantification and its application in functional foods: A review with a focus on immune enhancement activities and COVID-19. Front Nutr. 2021 Sep 21;8:747956. Go to original source... Go to PubMed...
    35. Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal activity of curcumin I is associated with damaging of bacterial membrane. PLoS One. 2015 Mar 26;10(3):e0121313. Go to original source... Go to PubMed...
    36. Urrutia A, Orellana L, Sierra KS, Reina M, Figueroa JC, Jackson AP, Macklin KS, Buhr RJ, Bourassa DV. In vitro effect of photoactive compounds curcumin and chlorophyllin against single strains of Salmonella and Campylobacter. J Food Prot. 2023 Nov;86(11):100157. Go to original source... Go to PubMed...
    37. Wang J, Vaddu S, Bhumanapalli S, Mishra A, Applegate T, Singh M, Thippareddi H. A systematic review and meta-analysis of the sources of Campylobacter in poultry production (preharvest) and their relative contributions to the microbial risk of poultry meat. Poult Sci. 2023 Oct;102(10):102905. Go to original source... Go to PubMed...
    38. Wang F, Xu, Z Chen L, Qiao Z, Hu Y, Fan X, Liu Y, Kang Z, Huang F, Han M, Yang H, Feng X. Super absorbent resilience antibacterial aerogel with curcumin for fresh pork preservation. Food Control. 2024 May;159:1-10. Go to original source...
    39. Zermeno-Ruiz M, Rangel-Castaneda IA, Suarez-Rico DO, Hernandez-Hernandez L, Cortes-Zarate R, Hernandez-Hernandez JM, Camargo-Hernandez G, Castillo-Romero A. Curcumin stimulates the overexpression of virulence factors in Salmonella enterica serovar Typhimurium: In vitro and animal model studies. Antibiotics (Basel). 2022 Sep 10;11(9):1230. Go to original source... Go to PubMed...

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