Vet Med - Czech, 2024, 69(8):281-296 | DOI: 10.17221/35/2024-VETMED

Probiotic bacteria of wild boar origin intended for piglets – An in vitro studyOriginal Paper

I Kostovova ORCID...1, K Kavanova ORCID...1, M Moravkova ORCID...1, J Gebauer2, L Leva2, M Vícenova ORCID...2, V Babak ORCID...1, M Faldyna ORCID...2, M Crhanova ORCID...1
1 Department of Microbiology and Antimicrobial Resistance, Veterinary Research Institute, Brno, Czech Republic
2 Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic

Using probiotics represents a potential solution to post-weaning diarrheal diseases in piglets on commercial farms. The gastrointestinal tract of wild boars serves as a promising reservoir of novel lactic acid bacteria with suitable probiotic characteristics. In this study, we isolated eight bacterial strains from the intestinal content of wild boars identified as representatives of the species Bifidobacterium apri, Lactobacillus amylovorus, and Ligilactobacillus salivarius. These isolates underwent in vitro analysis and characterisation to assess their biological safety and probiotic properties. Analysis of their full genome sequences revealed the absence of horizontally transferrable genes for antibiotic resistance. However, seven out of eight isolates harboured genes encoding various types of bacteriocins in their genomes, and bacteriocin production was further confirmed by mass spectrometry analysis. Most of the tested strains demonstrated the ability to inhibit the growth of selected pathogenic bacteria, produce exopolysaccharides, and stimulate the expression of interleukin-10 in porcine macrophages. These characteristics deem the isolates characterised in this study as potential candidates for use as probiotics for piglets during the post-weaning period.

Keywords: antibiotic susceptibility; antimicrobial activity; bacteriocins; exopolysaccharides; interleukin-10

Received: April 25, 2024; Revised: June 14, 2024; Accepted: June 26, 2024; Published: August 29, 2024  Show citation

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Kostovova I, Kavanova K, Moravkova M, Gebauer J, Leva L, Vícenova M, et al.. Probiotic bacteria of wild boar origin intended for piglets – An in vitro study. Vet Med - Czech. 2024;69(8):281-296. doi: 10.17221/35/2024-VETMED.
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