Veterinární medicína, 2002 (vol. 47), issue 6
Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods
J. Osek, P. Gallien
Vet Med - Czech, 2002, 47(6):149-158 | DOI: 10.17221/5819-VETMED 
Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC). Only two strains, both of cattle origin, were positive for the fliC (H7) gene and could be classified as O157 : H7. Nine isolates had stx shiga toxin genes, either stx1 (1 strain), stx2 (4 isolates) or both (4 strains). The stx2-carrying samples were further subtyped by PCR for the stx2c, stx2d, and stx2e toxin variants. It was shown that all but one stx2-positive...
Variations in environmental contamination by polychlorinated biphenyls (PCB) and chlorinated pesticides (Lindane, DDT) on ping farms in Hodonín district in 1994 to 1999
R. Ulrich, J. Raszyk
Vet Med - Czech, 2002, 47(6):159-168 | DOI: 10.17221/5820-VETMED
Environmental samples (n = 216) were collected at regular intervals from 1994 to 1999 on three pig farms (D., M., T.) in the area of the district town of Hodonín, Czech Republic. The samples of feed mixtures (n = 85), drinking water (n = 44), barn dust sediments (n = 45), and muscular tissue of feeder pigs (n = 42) were analysed for the concentrations of PCB (sum of 7 indicator congeners), DDT (sum of 6 isomers), and lindane (g-hexachlorocyclohexane). The concentrations of lindane showed increasing tendencies in feed mixtures (1995-1999), drinking water (1997-1999), barn dust sediments (1996-1998), and pork...
Lactic acid bacteria, probiotics and immune system
R. Herich, M. Levkut
Vet Med - Czech, 2002, 47(6):169-180 | DOI: 10.17221/5821-VETMED
Mucous membranes of the body are in direct contact with the outside environment and they are colonised by a large number of different bacteria. Through mucous membranes, the organism is in permanent con-tact with different antigens. Mucous surfaces are protected by many defence mechanisms that ensure a permanent and effective protection. They include the production of secretory IgA, the production of mucus, cytoprotective peptides, defensins etc. Indigenous microflora markedly affects the structure of the host mucous, its function, and the development of the whole immune system. Protective microflora prevents pathogens from adhering by competi-tion...