Veterinární medicína, 2005 (vol. 50), issue 6

Generation of hapten-specific recombinant antibodies: antibody phage display technology: a review

J. Brichta, M. Hnilova, T. Viskovic

Vet Med - Czech, 2005, 50(6):231-252 | DOI: 10.17221/5620-VETMED  

Production of antibodies has been revolutionized by the development of modern molecular biology methods for the expression of recombinant DNA. Phage display technology represents one of the most powerful tools for production and selection of recombinant antibodies and has been recognized as a valuable alternative way for the preparation of antibodies of a desired specificity. In comparison to poly- and monoclonal antibodies, recombinant antibodies using the phage display technology can be prepared faster, in more automatic process and with reduced consumption of laboratory animals. This review summarizes current trends of phage display technology with...

Physiological and behavioural effects of changeover from conventional to automatic milking in dairy cows with and without previous experience

D. Weiss, E. Moestl, R. M. Bruckmaier

Vet Med - Czech, 2005, 50(6):253-261 | DOI: 10.17221/5621-VETMED  

The effects of the changeover from conventional parlour to an automatic milking system (AMS) on behaviour and physiological parameters in dairy cows with and without previous experience in AMS milking were investigated. Heart rate increase was higher in unexperienced cows (UC) than in experienced cows (EC) during the first AMS visit (31 ± 2 and 12 ± 2 beats per min, respectively, P < 0.05). EC entered the automatic milking stall voluntarily without any intervention by the staff. In contrast, in UC the rate of voluntary visits was 4, 26, 40, 49, 63, 72, 76, 89, 91 and 94% during the first 10 d of AMS milking, respectively....

Detection of mycoplasma contamination in cell cultures and bovine sera

H. Dvorakova, L. Valicek, M. Reichelova

Vet Med - Czech, 2005, 50(6):262-268 | DOI: 10.17221/5622-VETMED  

Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most common contaminants of cell lines (Mycoplasma arginini, M. fermentans, M. hyorhinis, M. bovis, M. orale, M. hominis, and Acholeplasma laidlawii) were used to verify the method. Then we assessed five selected cell lines and three bovine sera by...

Effects of clove oil anaesthesia on common carp (Cyprinus carpio L.)

J. Velisek, Z. Svobodova, V. Piackova, L. Groch, L. Nepejchalova

Vet Med - Czech, 2005, 50(6):269-275 | DOI: 10.17221/5623-VETMED  

The aim of the study was to investigate acute toxicity of clove oil for common carp and, using values of haematological and biochemical profiles of blood and histological tissue examinations, to assess the effects of the fish exposure to that anaesthetic. Acute toxicity values of clove oil for carp were found 10 minLC50 74.3 mg/l; 10minLC0.1 51.6 mg/l; 10minLC99.9 110.1 mg/l; 96hLC50 18.10 mg/l; 96hLC0.1 15.45 mg/l; and 96hLC99.9 19.80 mg/l. The fish were divided into four groups for haematological and biochemical examinations of blood and histological examinations of tissues. The groups were Control I (before the anaesthetic administration), Experiment...

Co-infection with papillomavirus and Knemidokoptes jamaicensis (Acari: Knemidokoptidae) in a chaffinch (Fringilla coelebs) and a case of beak papillomatosis in another chaffinch

I. Literak, B. Smid, F. Dusbabek, R. Halouzka, L. Novotny

Vet Med - Czech, 2005, 50(6):276-280 | DOI: 10.17221/5624-VETMED  

Nodular papillomatous lesions combined with proliferation and formation of scales and crusts were observed on both tarsi of a dead chaffinch (Fringilla coelebs) found in February 2004 in the eastern part of the Czech Republic. Electron microscopic examination demonstrated the presence of papillomavirus virions in the lesions. The parasitological examination of crust scraps revealed the presence of mites Knemidokoptes jamaicensis. Skin lesions were caused by a co-infection with the two pathogens. This paper is the first report of such a co-infection. In another chaffinch netted in May 2004 in the northern part of the Czech Republic,...