Veterinární medicína, 2013 (vol. 58), issue 2
The structure and role of mammalian sperm RNA: a reviewReview
D. Bukowska, B. Kempisty, H. Piotrowska, P. Sosinska, M. Wozna, S. Ciesiolka, P. Antosik, J.M. Jaskowski, K.P. Brüssow, M. Nowicki
Vet Med - Czech, 2013, 58(2):57-64 | DOI: 10.17221/6696-VETMED 
The main role of sperm is the delivery of the paternal genome into the oocyte during fertilisation. However, several lines of evidence have indicated that mammalian spermatozoa contribute more than just their DNA, namely, they also deliver a large range of RNA molecules. Microarray analysis has revealed a complex population of 3000 different kinds of messenger RNA that are delivered to oocytes by sperm and ejaculated spermatozoa are estimated to contain about 0.015 pg of total RNA. Some of the transcripts encode proteins crucial for early embryo development. Messenger RNAs from sperm also help to protect the paternal genes, which have an integral role...
Causes and consequences of maternal age-related aneuploidy in oocytes: a reviewReview
A. Danylevska, J. Sebestova
Vet Med - Czech, 2013, 58(2):65-72 | DOI: 10.17221/6697-VETMED
Although a positive correlation between aneuploidy and maternal age was first reported almost a century ago, the underlying mechanisms remain mostly unknown. Different hypotheses regarding age-related aneuploidy rise have been presented, but so far none of them can explain its full mechanism. Age-related aneuploidy is more likely to result from complex events taking place during the entire period of germ cell development, than from the failure of one particular mechanism. Recent findings confirm that the spindle assembly checkpoint (SAC) does not control and correct kinetochore-microtubule attachments in oocytes, enabling further propagation of aneuploidy,...
Hydrocephalus in dogs: a reviewReview
P. Przyborowska, Z. Adamiak, M. Jaskolska, Y. Zhalniarovich
Vet Med - Czech, 2013, 58(2):73-80 | DOI: 10.17221/6698-VETMED
Hydrocephalus is a multifactoral disorder that was rarely diagnosed in dogs until the availability of advanced imaging techniques in veterinary practice. This article reviews recent advances in the understanding of canine hydrocephalus including pathogenesis, clinical symptoms, diagnostic methods, and treatment solutions. The advantages and disadvantages of USG, RTG, CT and MRI as advanced diagnostic methods are discussed. For now Low-field Magnetic Resonance Imaging is the most useful tool in investigating hydrocephalus. The recommended sequences for MRI are T1-weighting images Spin echo, Field echo 3D with TR 380-750 ms, TE 12-25 ms, slice thickness...
Intrafollicular LH administration in dairy heifers treated with a GnRH agonistOriginal Paper
J. Mala, J.-F. Beckers, N. Melo de Sousa, E. Indrova, M. Lopatarova, R. Dolezel, S. Cech
Vet Med - Czech, 2013, 58(2):81-86 | DOI: 10.17221/6700-VETMED
The aim of this study was to evaluate the effect of intrafollicular treatment (IFT) with different doses of luteinising hormone. Experimental heifers were treated with a single deslorelin implant to desensitise gonadotroph cells of the pituitary gland. Thereafter, follicular development was stimulated by exogenous FSH treatment. Intrafollicular treatment with 10, 5, 1 and 0.01 µg LH was performed on one single follicle while other follicles remained untreated. Human chorionic gonadotrophine (2000 UI) was administered intravenously as a control. Ovulation and development of the corpus luteum occurred after all intrafollicular treatments with 10...
Potential use of dexmedetomidine for different levels of sedation, analgesia and anaesthesia in dogsOriginal Paper
R.A. Ahmad, Amarpal, P. Kinjavdekar, H.P. Aithal, A.M. Pawde, D. Kumar
Vet Med - Czech, 2013, 58(2):87-95 | DOI: 10.17221/6699-VETMED
A combination of drugs may be preferred over the use of a single agent to induce deep sedation. A synergistic interaction between the drugs reduces the dose requirements of the drugs thereby minimising the unwanted side effects associated with each drug and improving recovery. The present study was undertaken to evaluate the suitability of dexmedetomidine and dexmedetomidine in combination with midazolam-fentanyl or midazolam-fentanyl-ketamine for different levels of sedation, analgesia and anaesthesia in dogs. In a prospective, blinded, randomised clinical trial, 12 mixed breed dogs were divided into three groups. Animals of Group I were injected...
Standardised uptake values of 2-deoxy-2-[18F]fluoro-d-glucose using PET/CT in normal catsOriginal Paper
Y.K. Cho, K.C. Lee
Vet Med - Czech, 2013, 58(2):96-104 | DOI: 10.17221/6701-VETMED
In this study we assessed the normal physiological and dynamic thoracoabdominal distribution of 18F fluorodeoxyglucose (18F-FDG) uptake and the standardized uptake values (SUVs) of the major parenchymal organs in five normal young adult domestic short haired cats. Dynamic PET data were acquired with a transaxial field-of-view (FOV) PET/CT scanner, Regions of interests (ROIs) were manually drawn over the left ventricular free wall, left ventricular blood pool, liver, spleen, and left and right renal cortices. The SUVs of these organs were calculated for 5-min frames over the 90 min acquisition. The uptake of 18F-FDG...
Description of peritoneal cavity dirofilariosis caused by Dirofilaria immitis (Filarioidea: Onchocercidae) in a dog: a case reportCase Report
C. Sim, H.C. Kim, H.Y. Son, J.Y. Jung, S.Y. Ryu, B.K. Park
Vet Med - Czech, 2013, 58(2):105-108 | DOI: 10.17221/6702-VETMED
We describe Dirofilaria immitis occurring in the peritoneal cavity of a dog from Korea. Two worms were found in the fat of the umbrical cord area during operation for an umbrical cord hernia in a dog (four year old, female, Maltese). In gross findings, the worms were slander white and measured 132 mm (female), 111 mm (male). The tail of the male was spirally coiled. In light microscopy, the uterus was filled with not fully developed eggs. Scanning electron microscopy revealed that the cuticule was smooth on the head but those of the tail exhibited complex cuticular striation in the male. Head papillae were located in a cephalic plate forming...
Chocolate ingestion-induced non-cardiogenic pulmonary oedema in a puppy: a case reportCase Report
C.F. Agudelo, Z. Filipejova, P. Schanilec
Vet Med - Czech, 2013, 58(2):109-112 | DOI: 10.17221/6703-VETMED
Chocolate intoxication in small animals may be life-threatening and associated with serious morbidity and mortality. The main clinical presentations are cardio-respiratory and neurological complications. One of the most common post-mortem findings is pulmonary oedema which can be a cause of immediate death. We report a case of non-cardiogenic pulmonary oedema thought to be triggered by chocolate intoxication in a three-month-old puppy Dachshund dog. To the authors' knowledge this is the first time such a complication has been reported after chocolate ingestion. The history, clinical signs, diagnostic approach (radiographs, methylxanthine concentrations,...