Vet Med - Czech, 2002, 47(5):117-121 | DOI: 10.17221/5814-VETMED

Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901

P. Svastova, I. Pavlik, M. Bartos
Veterinary Research Institute, Brno, Czech Republic

The aim of this study was to examine the specificity of primers designed to detect the insertion element IS901 commonly used in differentiation of Mycobacterium avium complex strains. This study shows that one of these primers non-specifically anneals to a sequence inside insertion element IS900, specific IS of M. avium subsp. paratuberculosis and to another sequence flanking this element. The resulting non-specific amplicon can be a product of amplification from some M. avium subsp. paratuberculosis strains and can simulate the presence of insertion element IS901 in these strains. However size difference between specific and non-specific amplicons allows such false-positive results to be distinguished. In addition the single PCR allows a rapid and simple differentiation between IS901+ M. avium subsp. avium and M. avium subsp. paratuberculosis strains.

Keywords: IS901; IS900; PCR; Johne's diseases; avian tuberculosis

Published: May 31, 2002  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Svastova P, Pavlik I, Bartos M. Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901. Vet Med - Czech. 2002;47(5):117-121. doi: 10.17221/5814-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bull T.J., Hermon-Taylor J., Pavlik I., El-Zaatari F., Tizard M. (2000): Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing. Microbiology, 146, 2185-2197. Go to original source... Go to PubMed...
    2. Chiodini R.J. (1989): Crohns disease and the mycobacteriosis: a review and comparision of two disease entities. Clin. Microbiol. Rev., 2, 90-117. Go to original source... Go to PubMed...
    3. Collins F.M., Stokes W. (1987): Mycobacterium avium complex infections in normal and immunodeficient mice. Tubercle, 68, 127-136. Go to original source... Go to PubMed...
    4. Harris N.B., Barletta R.G. (2001): Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clin. Microbiol. Rev., 14, 489-512. Go to original source... Go to PubMed...
    5. Hermon-Taylor J. (2000): Mycobacterium avium subsp. paratuberculosis in causation of Crohn's disease. World J. Gastroenterol., 6, 630-632. Go to original source... Go to PubMed...
    6. Hermon-Taylor J., Bull T. (2002): Crohn's disease caused by Mycobacterium avium subspecies paratuberculosis: a public health tragedy whose resolution is long overdue. J. Med. Microbiol., 51, 3-6. Go to original source... Go to PubMed...
    7. Kunze Z.M., Wall S., Appelberg R., Silva M.T., Portaels F., McFadden J.J. (1991): IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium. Mol. Microbiol., 5, 2265-2272. Go to original source... Go to PubMed...
    8. Kunze Z.M., Portaels F., McFadden J.J. (1992): Biological distinct subtypes of Mycobacterium avium differ in possession on insertion sequence IS901. J. Clin. Microbiol., 30, 2366-2372. Go to original source... Go to PubMed...
    9. Pavlik I., Svastova P., Bartl J., Dvorska L., Rychlik I. (2000): Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals, humans, and the environment and virulence for poultry. Clin. Diag. Lab. Immunol., 7, 212-217. Go to original source... Go to PubMed...
    10. Puyang X., Lee K. Pawlichuk C., Kunimoto D.Y. (1999): IS1626, a new IS900-related Mycobacterium avium insertion sequence. Microbiol., 145, 3163-3168. Go to original source... Go to PubMed...
    11. Roiz M.P., Palenque E., Guerrero C., Gacria M.J. (1995): Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains. J. Clin. Microbiol., 33, 1389-1391. Go to original source... Go to PubMed...
    12. Thorel M.F., Huchzermeyer H., Weiss R., Fontaine J.J. (1997): Mycobacterium avium infections in animals. Vet. Res., 28, 439-447.

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content