Veterinární medicína, 2002 (vol. 47), issue 5
Rapid differentiation of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis by amplification of insertion element IS901
P. Svastova, I. Pavlik, M. Bartos
Vet Med - Czech, 2002, 47(5):117-121 | DOI: 10.17221/5814-VETMED 
The aim of this study was to examine the specificity of primers designed to detect the insertion element IS901 commonly used in differentiation of Mycobacterium avium complex strains. This study shows that one of these primers non-specifically anneals to a sequence inside insertion element IS900, specific IS of M. avium subsp. paratuberculosis and to another sequence flanking this element. The resulting non-specific amplicon can be a product of amplification from some M. avium subsp. paratuberculosis strains and can simulate the presence of insertion element IS901...
Incidence of bovine tuberculosis in wild and domestic animals other than cattle in six Central European countries during 1990-1999
I. Pavlik, M. Machackova, W. Yayo Ayele, J. Lamka, I. Parmova, I. Melicharek, M. Hanzlikova, B. Körmendy, G. Nagy, Z. Cvetnic, M. Ocepek, M. Lipiec
Vet Med - Czech, 2002, 47(5):122-131 | DOI: 10.17221/5815-VETMED
The study was undertaken in Croatia, Czech Republic, Hungary, Poland, Slovakia and Slovenia laying between Baltic and Adriatic seas on 610 402 km2. Mycobacterium bovis infection was diagnosed in 70 animals belonging to 17 species other than cattle. The set of wild animals comprised 12 European bison (Bison bonasus), one red deer (Cervus elaphus), five wild boars (Sus scrofa), and one European wild goat (Capra aegagrus) bred in a game park. Further positive animals included two farmed red deer (Cervus elaphus) and one bactrian camel (Camelus ferus) owned by a circus. The infection...
Gene typing of the colonisation factors F18 of Escherichia coli isolated from piglets suffering from post-weaning oedema disease
P. Alexa, K. Štouraová, J. Hamík, E. Salajka
Vet Med - Czech, 2002, 47(5):132-136 | DOI: 10.17221/5816-VETMED
Production of verotoxin Stx2e and expression of F18 and K88 colonisation factors were investigated in 222 strains of Escherichia coli isolated from weaned piglets. Sixty-two, 30, and 11 of the 129 verotoxigenic strains were classified to the serogroups O139, O141, and O157, respectively. Other serogroups were identified only sporadically and sixteen strains were unclassifiable. No colonisation factors were detectable in 15 (24.2%) of the 62 verotoxigenic strains classified with the serogroup O139. The fedA gene shared by the colonisation factors F18 was detected by PCR in 47 of the O139 strains (all but one F18ab). Gene fedA,...
Exposure of pig fatteners and dairy cows to polycyclic aromatic hydrocarbons
M. Ciganek, R. Ulrich, J. Nea, J. Raszyk
Vet Med - Czech, 2002, 47(5):137-142 | DOI: 10.17221/5817-VETMED
Exposure of pig fatteners and dairy cows to polycyclic aromatic hydrocarbons (PAHs) was investigated by analyses of selected indoor and outdoor samples. PAH concentrations (16 U.S. EPA priority PAHs) and data on common exposure routes were used for exposure calculations. The samples under study included water (n = 24), feed (n = 48), indoor air (n = 15), barn dust (n = 17), outdoor air (n = 6) road dust (n = 17), and soil (n = 15) collected in the summer 1999 and in the spring 2000 on 3 pig and 2 dairy cattle farms. The following mean concentrations of 16 PAHs were found: 100 ng/l in...
Counter immunoelectrophoresis: a simple method for the detection of species-specific muscle proteins in heat-processed products
L. Necidová, E. Renová, I. Svoboda
Vet Med - Czech, 2002, 47(5):143-147 | DOI: 10.17221/5818-VETMED
Counter immunoelectrophoresis (CIE) was used for the detection of species-specific muscle proteins in food products. This technique allowed the detection of pork, beef, poultry, or and kangaroo meats in heat-processed products at concentrations below 1.5%. CIE is based on the use of species-specific polyclonal antibodies prepared by immunisation of rabbits with heat-stable antigens extracted from visibly fat-free muscular tissue heated to 75°C, 100°C, or 120°C for 30 minutes. Adulterations in terms of declared product compositions were demonstrated by this method in 7 of the 50 tested commercial products.