Vet Med - Czech, 2002, 47(5):143-147 | DOI: 10.17221/5818-VETMED

Counter immunoelectrophoresis: a simple method for the detection of species-specific muscle proteins in heat-processed products

L. Necidová, E. Renová, I. Svoboda
1 University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
2 Veterinary Research Institute, Brno, Czech Republic
3 Biovendor, Brno, Czech Republic

Counter immunoelectrophoresis (CIE) was used for the detection of species-specific muscle proteins in food products. This technique allowed the detection of pork, beef, poultry, or and kangaroo meats in heat-processed products at concentrations below 1.5%. CIE is based on the use of species-specific polyclonal antibodies prepared by immunisation of rabbits with heat-stable antigens extracted from visibly fat-free muscular tissue heated to 75°C, 100°C, or 120°C for 30 minutes. Adulterations in terms of declared product compositions were demonstrated by this method in 7 of the 50 tested commercial products.

Keywords: counter-immunoelectrophoresis; muscle protein; thermostable antigen; antiserum; heat-processed meat products

Published: May 31, 2002  Show citation

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Necidová L, Renová E, Svoboda I. Counter immunoelectrophoresis: a simple method for the detection of species-specific muscle proteins in heat-processed products. Vet Med - Czech. 2002;47(5):143-147. doi: 10.17221/5818-VETMED.
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