Vet Med - Czech, 2003, 48(12):359-362 | DOI: 10.17221/5790-VETMED

Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey

G. Ozbes, H.B. Ertas, A. Muzo
Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey

Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay. The assay amplifies a 743 bp fragment of the IBDV VP2 gene. The RFLP profiles of 40 of these positive samples were determined using the enzyme MboI. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity exists among IBDV strains in a infected flock.

Keywords: IBDV; RT, PCR; commercially reared chickens

Published: December 31, 2003  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Ozbes G, Ertas HB, Muzo A. Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey. Vet Med - Czech. 2003;48(12):359-362. doi: 10.17221/5790-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akin A., Wu C.C., Lin T.L. (1998): A comparison of two RNA isolation methods for double-stranded RNA of infectious bursal disease virus. J. Virol. Methods, 74, 179-184. Go to original source... Go to PubMed...
    2. Banda A., Villegas P., El-A rache J., Estevez C. (2001): Molecular characterization of seven field isolates of infectious bursal disease virus obtained from commercial broiler chickens. Avian Dis., 45, 620-630. Go to original source... Go to PubMed...
    3. Dormitorio T.V., Giambrone J.J., Duck L.W. (1997): Sequence comparisons of the variable VP2 region of eight infectious bursal disease virus isolates. Avian Dis., 41 (1), 36-44. Go to original source... Go to PubMed...
    4. Dybing J.K., Jackwood D.J. (1996): Restriction analysis of the MD infectious bursal diseas evirus strain. Avian Dis., 40, 931-937. Go to original source... Go to PubMed...
    5. Jackwood D.J., Jackwood R.J. (1994): Infectious bursal disease viruses: molecular differentiation of antigenic subtypes among serotype 1 viruses. Avian Dis., 38, 531-537. Go to original source... Go to PubMed...
    6. Jackwood D.J, Jackwood R.J. (1997): Molecular identification of infectious bursal disease virus strains. Avian Dis., 41, 97-104. Go to original source... Go to PubMed...
    7. Jackwood D.J., Nielsen C.K. (1997): Detection of infectious bursal disease viruses in commercially reared chickens using the reverse transcriptase/polymerase chain reaction-restriction endonuclease assay. Avian Dis., 41, 137-143. Go to original source... Go to PubMed...
    8. Jackwood D.J., Sommer S.E. (1997): Restriction fragment length polymorphisms in the VP2 gene of infectious bursal disease viruses. Avian Dis., 41, 627-637. Go to original source... Go to PubMed...
    9. Jackwood D.J., Sommer S.E. (1998): Genetic heterogeneity in the VP2 gene of infectious bursal disease viruses detected in commercially reared chickens. Avian Dis., 42, 321-339. Go to original source... Go to PubMed...
    10. Jackwood D.J., Sommer S.E. (1999): Restriction fragment length polymorphisms in the VP2 gene of infectious bursal disease viruses from outside the United States. Avian Dis., 43, 310-314. Go to original source... Go to PubMed...
    11. Jackwood D.J., Byerley E.H., Sommer S.E. (2001): Use of a genetic marker for wild-type potentially pathogenic infectious bursal disease viruses. Avian Dis., 45, 701-705. Go to original source... Go to PubMed...
    12. Lin Z., Kato A., Otaki Y., Nakamura T., Sasmaz E., Ueda S. (1993): Sequence comparisons of a highly virulent

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content