Veterinární medicína, 2003 (vol. 48), issue 12
Mycobacteria isolated from the environment of pig farms in the Czech Republic during the years 1996 to 2002
L. Matlova, L. Dvorska, J. Bartl, M. Bartos, W.Y. Ayele, M. Alexa, I. Pavlik
Vet Med - Czech, 2003, 48(12):343-357 | DOI: 10.17221/5789-VETMED 
Sources of mycobacterial infections in 50 pig herds in the Czech Republicwere investigated during the years 1996 to 2002. A total of 2 412 samples from the external environment (feeds, bedding materials, drinking water, biofilms on drinkers, scrapings from the walls, floors and pen barriers, dust, spider webs, peat, kaolin, faeces, organs of rodents, and birds, etc.) were examined. After staining by the Ziehl-Neelsen technique, acid-fast rods were detected in 95 (3.9%) samples by direct microscopic examination and mycobacteria were cultured from 575 (23.8%) samples. From Mycobacterium avium complex (MAC), M. avium subsp....
Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey
G. Ozbes, H.B. Ertas, A. Muzo
Vet Med - Czech, 2003, 48(12):359-362 | DOI: 10.17221/5790-VETMED
Infectious bursal disease Virus (IBDV) specific reverse transcriptase/polymerase chain recation (RT/PCR) positive 40 broiler bursa fabricius samples obtained from a commercially reared flock were investigated for genetic diversity by PCR-RFLP assay. The assay amplifies a 743 bp fragment of the IBDV VP2 gene. The RFLP profiles of 40 of these positive samples were determined using the enzyme MboI. Most of the viruses had the same RFLP with the MboI enzyme. RFLP analysis of the isolates produced two different band profiles. The results of this study showed that little genetic heterogeneity exists among IBDV strains in a infected flock.
Polychlorinated biphenyls in raptor and owl eggs in the Czech Republic
I. Kubistova, M. Vavrova, I. Literak
Vet Med - Czech, 2003, 48(12):363-368 | DOI: 10.17221/5791-VETMED
In 2001, raptor and owl eggs were collected for the purpose of detection of contamination by polychlorinated biphenyls (PCBs) at 15 sites in the southeastern area of the Czech Republic. In total 31 unhatched eggs of 4 raptor species and 3 owl species were examined. PCB determination was carried out by high-resolution gas chromatography. PCB content was expressed in μg per 1 kg of lipid weight for the major PCB congeners 28, 52, 101, 118, 138, 153 and 180, serving as indicators. In all examined eggs the indicator PCB congeners were found. The ranges of total values of indicator PCB congeners found in different raptor and owl species were as follows:...
Immunohistochemical localization of calbindin-D28k in the kidney and cerebellum of the porcupines (Hystrix cristata)
S. Timurkaan, A. Aydin, M. Kaban
Vet Med - Czech, 2003, 48(12):369-372 | DOI: 10.17221/5792-VETMED
The localization of calbindin in the kidney and cerebellum of Hystrix cristata was investigated immunohistochemically using an antiserum against the 28k Da calbindin of chicken duodenum. Calbindin-D28k is an intracellular protein with a high affinity for calcium. This protein is exclusively localized in the distal convoluted tubules of the kidney and in Purkinje cells of the cerebellum. Functionaly, calbindin-D28k is supposed to be involved in the regulation of the reabsorption of calcium in the distal nephron, though the exact regulatory mechanisms are not yet known.
Defective wing development in black-headed gull (Larus ridibundus) - case report
J. Rajchard, V. Rajchard
Vet Med - Czech, 2003, 48(12):373-374 | DOI: 10.17221/5793-VETMED
In 2000-2001 juvenile individuals of black-headed gull (Larus ridibundus) were repeatedly found in a Nadeje fishpond system in the Trebon Basin Area that were incapable of flying, with deformed wings. The investigation proved the rotation of metacarpal bones and luxation of the carpal joint. The Roentgen photograph did not show any consequences of any fracture, but this was evidently a post-traumatic state. A possible explanation is traumatisation of developing wings in young birds that got stuck by their wings in viscous mud around the nest colony.