Veterinární medicína, 2026 (vol. 71), issue 6
Antimicrobial resistance in veterinary medicine: Mechanisms, regulation and control strategiesReview
P Matlakova, K Nedbalcova
Vet Med - Czech, 2026, 71(6):209-221 | DOI: 10.17221/2/2026-VETMED 
Antimicrobial resistance (AMR) represents a major global threat to both human and animal health. Within the One Health framework, farm and wild animals serve as critical reservoirs of resistant bacteria and resistance genes, which can disseminate through food, water, and the environment. The excessive and inappropriate use of antibiotics in veterinary medicine exerts selection pressure that fosters the emergence and spread of antimicrobial resistance. Current mitigation strategies aim to reduce antibiotic consumption and promote prudent use through hygiene measures, vaccination, individualised therapy, pharmacokinetic-pharmacodynamic (PK/PD)...
Meloxicam blood levels are not affected by sex-related hormones in male and female goatsOriginal Paper
A Gokceoglu, Z Ozdemir Kutahya, GF Yarim, P Piner Benli, C Gokbulut
Vet Med - Czech, 2026, 71(6):222-229 | DOI: 10.17221/103/2025-VETMED
The present study aims to investigate the interaction between the non-steroidal anti-inflammatory (NSAID) drug meloxicam and sex hormones in seasonal polyoestrous male and female Saanen goats. It was hypothesised that sex hormones, both steroidal and peptide hormones, may influence the serum concentration of the lipophilic drug meloxicam. In the study, the relationship between plasma meloxicam concentration (μg/ml) and the levels of follicle-stimulating hormone (FSH), anti-Müllerian hormone (AMH), testosterone, oestradiol (E2), and progesterone (P4) was evaluated. For this purpose, six male and six female Saanen goats aged one year were...
Impact of histological and molecular subtype on the potential therapeutic effect of buparlisib in canine mammary gland tumoursOriginal Paper
A Baykal Ugur, G Guney Eskiler, O Turna
Vet Med - Czech, 2026, 71(6):230-244 | DOI: 10.17221/48/2025-VETMED
This study aims to evaluate the response of primary cells to buparlisib, a PI3K inhibitor, at varying concentrations and exposure durations across different histological and molecular subtypes of CMGTs, and to assess PI3K/Akt/mTOR pathway activity by measuring Akt and mTOR expression. Three carcinomas (C), three sarcomas (S), and two carcinosarcomas (CS) tumours were collected from the dogs. The primary cells were produced from tissues and treated with buparlisib at different doses. Subsequently, the WST-1 assay, Annexin V, and AO/PI staining were performed sequentially to evaluate the effects of buparlisib. PI3K/Akt/mTOR signalling pathway inhibition...
A reproducible rat model for warfarin studies: Prothrombin time assessment with minimal blood using the CoaguChek XS and determination of an effective warfarin dosageOriginal Paper
ET Akyol, MT Hekim, M Erol
Vet Med - Czech, 2026, 71(6):245-253 | DOI: 10.17221/4/2026-VETMED
Warfarin research in rats is often challenged by methodological inconsistencies and large blood volume requirements. This study aimed to validate a refined, reproducible rat model for serial prothrombin time (PT) monitoring using the CoaguChek XS, in alignment with the 3R principles, and to determine an effective warfarin sodium dosage. Male Wistar albino rats received daily oral warfarin (0.125–0.750 mg/kg) for 5 days. PT was measured using the whole-blood CoaguChek XS and two laboratory plasma assays (Innovin, Thromborel S). Agreement was assessed using Bland–Altman and Passing–Bablok regression. The CoaguChek XS consistently yielded...