Veterinární medicína, 2026 (vol. 71), issue 8
Microplastic-induced changes in the rainbow trout gut: Insights from transcriptomic, proteomic, and microbial approachesOriginal Paper
N Hodkovicova, A Hollerova, J Gebauer, M Crhanova, K Stastny, Z Svobodova, M Faldyna
Vet Med - Czech, 2026, 71(8):295-309 | DOI: 10.17221/85/2025-VETMED 
This study examined the intestinal effects of polyethylene (PE; size 46.6 ± 11.3 µm) and polystyrene (PS; size 52.5 ± 11.5 µm) microparticles at three different concentrations (0.5%, 2%, and 5%) on juvenile rainbow trout (Oncorhynchus mykiss) using an integrative approach combining transcriptomics, proteomics, and microbiome analysis. After a six-week experiment, molecular analyses identified concentration-dependent transcriptional responses, including downregulation of genes involved in ion exchange (slc9a1b) and appetite regulation (ghrl), alongside upregulation of immune- and stress-related genes (il10,...
Susceptibility of different rainbow trout (Oncorhynchus mykiss) genetic lines to infectious diseaseOriginal Paper
I Papezikova, V Vaibarova, L Pojezdal, K Matejickova, J Blahova, J Mendel, E Maresova, I Mikulikova, M Palikova, I Toulova
Vet Med - Czech, 2026, 71(8):310-321 | DOI: 10.17221/100/2025-VETMED
This study assessed the resistance of three rainbow trout (Oncorhynchus mykiss) genetic lines to three pathogens, the bacterium Flavobacterium psychrophilum and the parasites Ichthyophthirius multifiliis and Tetracapsuloides bryosalmonae, by monitoring mortality, immune parameters (leukocyte count and phagocytic activity), and pathogen load. Two of the fish lines tested carried quantitative trait loci associated with resistance to the selected pathogens. Unexpectedly, the line with declared genetic resistance exhibited the highest mortality rate following F. psychrophilum infection, suggesting the influence...
The efficacy of sap obtained from Commiphora gileadensis against cadmium-induced hepatorenal toxicity in male ratsOriginal Paper
ASM Aljohani, S Al Dakhil, AS Soliman, M El-Alreshoodi, IM El-Ashmawy
Vet Med - Czech, 2026, 71(8):322-336 | DOI: 10.17221/86/2025-VETMED
Cadmium (Cd), a toxic heavy metal, ranks among the top ten environmental threats to human health, adversely affecting vital organs such as the central nervous system, kidneys, liver, pancreas, lungs, and testes. The antioxidant potentials of Commiphora gileadensis (CG) sap in vitro were estimated by the total phenolic and flavonoid content, DPPH (2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity) and ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) free radical scavenging. The reducing activities were also assessed using copper (cupric reducing antioxidant capacity, CUPRAC) and ferric (ferric reducing antioxidant...
Effects of vitamins A and E on eprinomectin transport in Caco-2 cellsOriginal Paper
Y Gunes, Y Gultekin, O Dogan, C Anlas, O Ustuner, N Yilmaz, T Bakirel
Vet Med - Czech, 2026, 71(8):337-347 | DOI: 10.17221/8/2026-VETMED
Eprinomectin (EPR) is a macrocyclic lactone antiparasitic widely used in veterinary medicine. Although the off-label oral use of the pour-on formulation of EPR is known to be effective and practical, data regarding its interactions with foods or vitamin supplements that may affect intestinal absorption remain limited. This study investigated the effects of vitamin A (VA) and vitamin E (VE) on the transepithelial permeability of EPR using the Caco-2 cell monolayer model. An analytical method for EPR quantification was developed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The integrity of tight junction dynamics...