Vet Med - Czech, 2005, 50(5):219-224 | DOI: 10.17221/5618-VETMED

Cryoprotection of porcine cornea: a scanning electron microscopy study

A. Neronov1, P. Giurov2, M. Cholakova1, M. Dimitrova1, E. Nikolova1
1 Institute of Experimental Morphology and Anthropology, Bulgarian Academy of Sciences, Sofia, Bulgaria
2 Institute of Experimental Pathology and Parasitology, Bulgarian Academy of Sciences, Sofia, Bulgaria

Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5-10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.

Keywords: porcine cornea; Me2SO; glycerol; 1,2-propanediol; PEG-400

Published: May 31, 2005  Show citation

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Neronov A, Giurov P, Cholakova M, Dimitrova M, Nikolova E. Cryoprotection of porcine cornea: a scanning electron microscopy study. Vet Med - Czech. 2005;50(5):219-224. doi: 10.17221/5618-VETMED.
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