Veterinární medicína, 2005 (vol. 50), issue 5

Monitoring of antibiotic resistance in isolates of Actinobacillus pleuropneumoniaein the Czech Republic between 2001 and 2003

K. Nedbalcova, P. Satran, Z. Jaglic, R. Ondriasova, Z. Kucerova

Vet Med - Czech, 2005, 50(5):181-185 | DOI: 10.17221/5613-VETMED  

During the period of 2001 and 2003 a total of 238 isolates of Actinobacillus pleuropneumoniae obtained from 26 pig herds in the Czech Republic (2001 - 73 isolates, 2002 - 110 isolates, 2003 - 55 isolates) were examined for antibiotic resistance by disc diffusion method. Resistance to erythromycin (95.5% to 98.6%), streptomycin (76.4% to 82.7%) and tiamulin (72.7%) was detected most frequently over the whole monitored period. High increase in percentage of resistant isolates to tetracycline (2001 - 11.0%, 2002 - 36.4%, 2003 - 81.8%), doxycycline (2001 - 9.6%, 2002 - 34.6%, 2003 - 61.8%), nalidixic acid (2001 - 2.7%, 2002 - 14.5%, 2003 - 45.5%),...

Effects of increased iodine supply on the selenium status of kids

L. Pavlata, S. Slosarkova, P. Fleischer, A. Pechova

Vet Med - Czech, 2005, 50(5):186-194 | DOI: 10.17221/5614-VETMED  

The aim of the study was to monitor the effect of increased iodine supplementation of the clinically healthy kid organism on the selenium status. The study included 7 clinically healthy 14-day-old kids from mothers with high iodine supplementation (group E) and 7 clinically healthy kids from mothers with hypoiodaemia (group C). Kids in group E were administered potassium iodide orally from 14 to 90 days of age. During the experimental period, the group E kids had a total daily iodine intake (from the feeding ration and from the per os potassium iodide administration) of 440-590 µg per head and day in comparison with 140-190 µg...

Using the Sperm Quality Analyzer (SQA IIc) to evaluate dog ejaculates

P. Prinosilova, Z. Veznik, A. Zajicova, D. Svecova

Vet Med - Czech, 2005, 50(5):195-204 | DOI: 10.17221/5615-VETMED  

38 fresh ejaculates were examined, of which 23 were cryopreserved in three diluents differing in concentration of glycerol and examined after thawing. For each ejaculate we established the concentration, motility, speed of sperm, percentage of live sperm and the morphological image of the ejaculate using routine laboratory diagnosis and SQA parameters at the initial value and after a 120-minute survival test. The values of the parameters found through routine diagnosis and using the SQA device were statistically confronted. From the results it emerges that the device functions on the basis of the turbulence effect of the semen, and therefore on the...

A flexible and precise model for the dynamics of animal diseases with single outbreaks

W.G. Nowak

Vet Med - Czech, 2005, 50(5):205-212 | DOI: 10.17221/5616-VETMED  

This article provides a general and accurate mathematical model for the epidemics dynamics of a large class of animal diseases. The issue is to offer to the scientist interested in applications a quite concise and practicable explanation how to validitate the model parameters by means of the software tool Microsoft Excel®. Furthermore, predictions concerning hypothetical scenarii can easily be obtained on the basis of this concept. As specific examples, calculations are presented for the BSE epidemics in the British UK and for an outbreak of foot-and-mouth disease in a district of Lower Austria.

Chewing lice (Phthiraptera: Amblycera, Ischnocera) from chukars (Alectoris chukar) from a pheasant farm in Jinacovice (Czech Republic)

O. Sychra

Vet Med - Czech, 2005, 50(5):213-218 | DOI: 10.17221/5617-VETMED  

One hundred and twenty captive chukars (Alectoris chukar) and other hosts from a pheasant farm in Jinacovice (Czech Republic) were searched for chewing lice from July 2003 to April 2004. Seven species of chewing lice were found on chukars: Amyrsidea perdicis, Menacanthus pallidulus, Menopon gallinae (Amblycera); Goniodes colchici, Lipeurus maculosus, Goniocotes microthorax and Cuclotogaster heterographus (Ischnocera). Except for the last two mentioned species, the other five louse species are recorded for the first time on chukars. The prevalence, mean intensity of infestation and mean...

Cryoprotection of porcine cornea: a scanning electron microscopy study

A. Neronov, P. Giurov, M. Cholakova, M. Dimitrova, E. Nikolova

Vet Med - Czech, 2005, 50(5):219-224 | DOI: 10.17221/5618-VETMED  

Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5-10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.

Immunohistochemical distribution of prolactin containing cells in the pituitary of the chickens

H. Erdost

Vet Med - Czech, 2005, 50(5):225-229 | DOI: 10.17221/5619-VETMED  

The objective of the current study was to determine the immunohistochemical distribution and density of prolactin containing cells in the pituitary of the female and male chickens during postnatal developing period after hatching for five months. The modified labelled avidin-biotin method with monoclonal mouse antihuman prolactin as a primary antibody was used to detect prolactin positive cells. At the end of each month, prolactin containing cells were identified in the pars distalis of the pituitary glands of chickens. The number of the prolactin positive cells were found to be increased depending on the monthly development period, while the reaction...