Vet Med - Czech, 2005, 50(5):195-204 | DOI: 10.17221/5615-VETMED

Using the Sperm Quality Analyzer (SQA IIc) to evaluate dog ejaculates

P. Prinosilova, Z. Veznik, A. Zajicova, D. Svecova
Veterinary Research Institute, Brno, Czech Republic

38 fresh ejaculates were examined, of which 23 were cryopreserved in three diluents differing in concentration of glycerol and examined after thawing. For each ejaculate we established the concentration, motility, speed of sperm, percentage of live sperm and the morphological image of the ejaculate using routine laboratory diagnosis and SQA parameters at the initial value and after a 120-minute survival test. The values of the parameters found through routine diagnosis and using the SQA device were statistically confronted. From the results it emerges that the device functions on the basis of the turbulence effect of the semen, and therefore on the intensity and character of the moving mass of sperm. From a research perspective, the SQA device is less usable, since the values it provides are not absolutely comparable with the values found through routine laboratory diagnosis. For the practitioner, however, the device may be useful, since the value of the sperm motility index (SMI) indicates the quality of the semen through the close connection of two parameters, concentration and motility. For a more precise assessment of the ejaculate, however, it is necessary to also analyse the remaining parameters of routine laboratory sperm analysis. To be useful in clinical practice for the evaluation of semen, it would be necessary to establish SMI limit values for ejaculates of varying quality.

Keywords: dog; ejaculate; sperm analysis; Sperm Quality Analyzer (SQA)

Published: May 31, 2005  Show citation

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Prinosilova P, Veznik Z, Zajicova A, Svecova D. Using the Sperm Quality Analyzer (SQA IIc) to evaluate dog ejaculates. Vet Med - Czech. 2005;50(5):195-204. doi: 10.17221/5615-VETMED.
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    References

    1. Andersen K. (1975): Insemination with frozen dog semen based on a new insemination technique. Zuchthygiene, 10, 1. Go to original source... Go to PubMed...
    2. Baker F., Gragle R.G., Salisbury G.W., Van Demark N.L. (1957): Spermatozoa velocities in vitro. A simple method of measurement. Fertility and Sterility, 8, 149-155. Go to original source... Go to PubMed...
    3. Bartoov B., Ben-Barak J., Mayesky A., Sneider M., Yogev L., Lightman A. (1991): Sperm motility index: a new parameter for human sperm evaluation. Fertility and Sterility, 56, 108-112. Go to original source... Go to PubMed...
    4. Comodo F., Inaudi P.,Petrilli S., D´Antona N. (1997): Sperm quality analyzer (SQA): A reliable tool for semen parameters evaluation. In: Proc. 10th World Congress for In Vitro Fertilization and Assisted Reproduction, Vancouver, Canada.
    5. Fukui Y., Togawa M., Abe N., Takano Y., Asada M., Okada A., Iida K., Ishikawa H., Ohsumi S. (2004): Validation of the sperm quality analyzer and the hypo-osmotic swelling test for frozen-thawed ram and minke whale (Balaenoptera bonarensis) spermatozoa. Journal of Reproduction and Development, 50, 147-154. Go to original source... Go to PubMed...
    6. Gamcik P., Kozumplik J., Schwarc F., Vlcek Z., Zibrin M. (1976): Artificial Insemination and Andrology in Livestock (in Czech). Príroda, Bratislava. 574 pp.
    7. Iguer-Ouada M., Verstegen JP. (2001): Validation of the sperm quality analyzer (SQA) for dog sperm analysis. Theriogenology, 55, 1143-1158. Go to original source... Go to PubMed...
    8. Johnston R.C., Clarke G.N., Liu D.Y., Baker H.W. (1995): Assessment of the sperm quality Analyzer. Fertility and Sterility, 63, 1071-1076. Go to original source... Go to PubMed...
    9. Kruger T.F., Menkveld R., Stander F.S.H., Lombard C.J., Van der Merve J.P., Van Zyl J.A., Smith K. (1986): Sperm morphologic features as a prognostic factor in vitro fertilization. Fertility and Sterility, 46, 1118-1123. Go to original source... Go to PubMed...
    10. Matouskova O., Cigler M., Chalupa J., Hruska K. (1992): STAT Plus - Manual (in Czech). 1st ed. Veterinary Research Institute, Brno. 168 pp.
    11. Neuman S.L., Mcdaniel C.D., Frank L., Radu J., Hester N. P.Y. (2002): Use of a sperm quality analyser on semen of turkey breeders to monitor storage time effects and age-related changes during a reproductive cycle. British Poultry Science, 43, 465-471. Go to original source... Go to PubMed...
    12. Rijsselaere T., Van Soom A., Maes D., DeKruif A. (2002): Use of the Sperm Quality analyzer (SQA II-C) for the Assessment of Dog Sperm Quality. Reproduction in Domestic Animals, 37, 158-163. Go to original source... Go to PubMed...
    13. Shibahara H., Suzuki T., Obara H., Hirano Y., Onagawa T., Taneichi A., Takamizawa S., Sato I. (2002): Accuracy of the normal sperm morphology value by Sperm Quality analyzer IIC: comparison with the strict criteria. International Journal of Andrology, 25, 45. Go to original source... Go to PubMed...
    14. Schieferstein G., Hook-Vervier B., Schwarz M. (1998): Sperm motility index. Archives of Andrology, 40, 43-48. Go to original source... Go to PubMed...
    15. Suzuki T., Shibahara H., Tsunoda H., Hirano Y., Taneichi A., Obara H.,Takamizawa S., Sato I. (2002): Comparison of the Sperm Quality Analyzer IIC variables with the computer-aided sperm analysis estimates. International Journal of Andrology, 25, 49. Go to original source... Go to PubMed...
    16. Veznik Z., Matouskova O., Svecova, Zajicova A. (2001): The use of the computer technology for the evaluation of the strict morphological sperm analysis. Veterinarni Medicina, 46, 35-40. Go to original source...
    17. Veznik Z., Svecova D., Zajicova A., Bendova J., Faldikova L., Zraly Z., Netolicka S., Pospisil L., Diblikova I., Zudova D., Matouskova O. (2000): Evaluation of semen for assisted reproduction and selection of breeding males - Strict analysis of sperm morphology SASMO (in Czech). Veterinary Research Institute, Brno. 105 pp.
    18. Veznik Z., Svecova D., Zajicova A., Prinosilova P. (2003): Functional evaluation of dog ejaculates with a priority aspect of acrosome integrity. Veterinarni Medicina, 48, 221-228. Go to original source...
    19. Veznik Z., Svecova D., Zajicova A., Prinosilova P. (2005): Repetitorium of spermatology and andrology and methods of sperm analysis (in Czech). Veterinary Research Institute, Brno. 197 pp.
    20. Vyt P., Maes D., Rijsselaere T., Dejonckheere E., Castryck F., Van Soom A. (2004): Motility assessment of porcine spermatozoa: a comparison of methods. Reproduction in Domestic Animals, 39, 447-453. Go to original source... Go to PubMed...

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