Vet Med - Czech, 2006, 51(5):224-231 | DOI: 10.17221/5541-VETMED

Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals

A. Pauciullo1, S. Kubickova2, H. Cernohorska2, K. Petrova2, D. Di Berardino1, L. Ramunno1, J. Rubes2
1 Department of Animal Science and Food Inspection, University of Naples "Federico II", via Portici (NA), Italy
2 Veterinary Research Institute, Brno, Czech Republic

In this study, new classes of tandemly repeated DNA sequences in the centromeric regions of three farm animal species are reported. Pericentromeric regions of bovine chromosome X, porcine chromosome 14 and equine chromosome 1 were microdissected by laser and amplified by DOP-PCR. Painting probes showed strong hybridization signals on their corresponding centromeres. Specific DNA fragments were isolated by cloning and sequenced. For each species, high homology level was found among the sequences. The presence of specific repeats within each sequence allows us to consider them as monomeric units or parts of longer tandemly repeated monomeric units that generate a specific higher-order repeat. In this respect, the isolation of new centromeric sequences enriches the genetic map of these three species and represents a useful tool for FISH analysis, thus expanding our knowledge on centromeres of farm animal species.

Keywords: Bos taurus; Sus scrofa; Equus caballus; cattle; pig; horse; centromeres; tandem repeats; chromosomes; FISH

Published: May 31, 2006  Show citation

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Pauciullo A, Kubickova S, Cernohorska H, Petrova K, Di Berardino D, Ramunno L, Rubes J. Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals. Vet Med - Czech. 2006;51(5):224-231. doi: 10.17221/5541-VETMED.
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    References

    1. Alexandrov I., Kazakov A., Tumeneva I., Shepelev V., Yurov Y. (2001): α-Satellite DNA of primates: old and new families. Chromosoma, 110, 253-266. Go to original source... Go to PubMed...
    2. Broad T.E., Forrest J.W., Lewis P.E., Pearce P.D., Phua S.H., Pugh P.A., Stewart-Scott I.A. (1995): Cloning of a DNA repeat element from horse: DNA sequence and chromosomal localization. Genome, 38, 1132-1138. Go to original source... Go to PubMed...
    3. Buckland R.A. (1985): Sequence and evolution of related bovine and caprine satellite DNAs. Identification of a short DNA sequence potentially involved in satellite DNA amplification. Journal of Molecular Biology, 186, 25-30. Go to original source... Go to PubMed...
    4. Buntjer J.B., Nijman I.J., Zijlstra C., Lenstra J.A. (1998): A satellite DNA element specific for roe deer (Capreolus capreolus). Chromosoma, 107, 1-5. Go to original source... Go to PubMed...
    5. Burke R.D., Szabo P., O'Brien S., Nash W.G., Yu L., Smith K.D. (1985): Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA. Chromosoma, 92, 225-233. Go to original source... Go to PubMed...
    6. Chaves R., Guedes-Pinto H., Heslop-Harrison J., Schwarzacher T. (2000): The species and chromosomal distribution of the centromeric alpha-satellite I sequence from sheep in the tribe Caprini and other Bovidae. Cytogenetics and Cell Genetics, 91, 62-66. Go to original source... Go to PubMed...
    7. Chaves R., Adega F., Heslop-Harrison J.S., Guedes-Pinto H., Wienberg J. (2003): Complex satellite DNA reshuffling in the polymorphic t(1;29) Robertsonian translocation and evolutionarily derived chromosomes in cattle. Chromosome Research, 11, 641-648. Go to original source... Go to PubMed...
    8. Devilee P., Thierry R.F., Kievits T., Kolluri R., Hopman A.H., Willard H.F., Pearson P.L., Cornelisse C.J. (1988): Detection of chromosome aneuploidy in interphase nuclei from human primary breast tumors using chromosome specific repetitive DNA probes. Cancer Research, 48, 5825-5830.
    9. Di Berardino D., Vozdova M., Kubickova S., Cernohorska H., Coppola G., Coppola G.F., Enne G., Rubes J. (2004): Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus L.) X- and Y-painting probes. Molecular Reproduction and Development, 67, 108-115. Go to original source... Go to PubMed...
    10. Ekes C., Csonka E., Hadlaczky G., Cserpan I. (2004): Isolation, cloning and characterization of two major satellite DNA families of rabbit (Oryctolagus cuniculus). Gene, 343, 271-279. Go to original source... Go to PubMed...
    11. Gallagher P.C., Lear T.L., Bailey E. (2000): Sequence characteristics and chromosomal distribution of a DNA satellite, ES22, in genomes of the horse and other equids. http://www.ncbi.nlm.nih.gov(unpublished).
    12. Haaf T., Willard H.F. (1997): Chromosome-specific alpha satellite DNA from the centromere of chimpanzee chromosome 4. Chromosoma, 106, 226-232. Go to original source... Go to PubMed...
    13. Huang N.F., Gupta M., Varghese S., Rao S., Luke S. (2002): Detection of numerical chromosomal abnormalities in epithelial ovarian neoplasms by fluorescence in situ hybridization (FISH) and a review of the current literature. Applied Immunohistochemistry and Molecular Morphology, 10, 187-193. Go to original source... Go to PubMed...
    14. Iannuzzi L., Di Meo G.P., Perucatti A., Incarnato D., Schibler L., Cribiu E.P. (2000): Comparative FISH mapping of bovid X chromosomes reveals homologies and divergences between the subfamilies Bovinae and Caprinae. Cytogenetics and Cell Genetics, 89, 171-176. Go to original source... Go to PubMed...
    15. Jantsch M., Hamilton B., Mayr B., Schweizer D. (1990): Meiotic chromosome behaviour reflects levels of sequence divergence in Sus scrofa domestica satellite DNA. Chromosoma, 99, 330-335. Go to original source... Go to PubMed...
    16. Janzen M.A., Buoen L.B., Zhao F., Louis C.F. (1999): Characterization of a swine chromosome-specific centromeric high-order repeat. Mammalian Genome, 10, 579-584. Go to original source... Go to PubMed...
    17. Jobse C., Buntjer J.B., Haagsma N., Breukelman H.J., Beintema J.J., Lenstra J.A. (1995): Evolution and recombination of bovine DNA repeats. Journal of Molecular Evolution, 41, 277-283. Go to original source... Go to PubMed...
    18. Kolluri R.V., Manuelidis L., Cremer T., Sait S., Gezer S., Raza A. (1990): Detection of monosomy 7 in interphase cells of patiens with myeloid disorders. American Journal of Hematology, 33, 117-122. Go to original source... Go to PubMed...
    19. Kubickova S., Cernohorska H., Musilova P., Rubes J. (2002): The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals. Chromosome Research, 10, 571-577. Go to original source... Go to PubMed...
    20. Lee C., Ritchie D.B., Lin C.C. (1994): A tandemly repetitive, centromeric DNA sequence from the Canadian woodland caribou (Rangifer tarandus caribou): its conservation and evolution in several deer species. Chromosome Research, 2, 293-306. Go to original source... Go to PubMed...
    21. Maniatis T., Fritsch E.F., Sambrook J. (1982): Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
    22. Mitchell A.R., Gosden J.R., Miller D.A., (1985): p82H: a cloned sequence of the alphoid repeated DNA family found at the centromeres of all human chromosomes. Chromosoma, 92, 369-377. Go to original source... Go to PubMed...
    23. Moore L.E., Titenko-Holland N., Smith M.T. (1993): Use of fluorescent in situ hybridization to detect chromosome specific changes in exfoliated human bladder and oral mucosa cells. Environmental and Molecular Mutagenesis, 22, 130-137. Go to original source... Go to PubMed...
    24. Moyzis R.K., Albright K.L., Bertholdi M.F., Cram L.S., Deaven L.L., Hildebrand C.E., Joste N.E., Longmire J.L., Meyne J., Schwarzacherrobinson T. (1987): Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis. Chromosoma, 95, 375-386. Go to original source... Go to PubMed...
    25. Nijman I.J., Lenstra J.A. (2001): Mutation and recombination in cattle satellite DNA: a feedback model for the evolution of satellite DNA repeats. Journal of Molecular Evolution, 52, 361-371. Go to original source... Go to PubMed...
    26. Pellestor F., Girardet A., Coignet L., Andreo B., Charlieu J.P. (1996): Assessment of aneuploidy for chromosomes 8, 9, 13, 16, and 21 in human sperm by using primed in situ labeling technique. American Journal of Human Genetics, 58, 797-802.
    27. Plucienniczak A., Skowronski J., Jaworski J. (1982): Nucleotide sequence of bovine 1.715 satellite DNA and its relation to other bovine satellite sequences. Journal of Molecular Biology, 158, 293-304. Go to original source... Go to PubMed...
    28. Riquet J., Mulsant P., Yerle M., San Cristobal-Gaudy M., Le Tissier P., Milan D., Gellin J. (1996): Sequence analysis and genetic mapping of porcine chromosome 11 centromeric S0048 marker. Cytogenetics and Cell Genetics, 74, 127-132. Go to original source... Go to PubMed...
    29. Rogel-Gaillard C., Hayes H., Coullin P., Chardon P., Vaiman M. (1997a): Swine centromeric DNA repeats revealed by primed in situ (PRINS) labeling. Cytogenetics and Cell Genetics, 79, 79-84. Go to original source... Go to PubMed...
    30. Rogel-Gaillard C., Bourgeaux N., Save J.C., Renard C., Coullin P., Pinton P., Yerle M., Vaiman M., Chardon P. (1997b): Construction of a swine YAC library allowing an efficient recovery of unique and centromeric repeated sequences. Mammalian Genome, 8, 186-192. Go to original source... Go to PubMed...
    31. Sakagami M., Hirota K., Awata T., Yasue H. (1994): Molecular cloning of an equine satellite-type DNA sequence and its chromosomal localization. Cytogenetics and Cell Genetics, 66, 27-30. Go to original source... Go to PubMed...
    32. Willard H.F. (1985): Chromosome-specific organization of human α-satellite DNA. American Journal of Human Genetics, 37, 524-532.
    33. Willard H.F., Waye J.S. (1987): Hierarchical order in chromosome-specific human alpha satellite DNA. Trends in genetics, 3, 192-198. Go to original source...

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