Vet Med - Czech, 2006, 51(5):258-264 | DOI: 10.17221/5545-VETMED

γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation

M. Faldyna1, L. Leva1, Z. Sladek1,2, D. Rysanek1, M. Toman1
1 Veterinary Research Institute, Brno, Czech Republic
2 Mendel University of Agriculture and Forestry, Brno, Czech Republic

γδ-T-Cell Receptor (TCR) lymphocytes were detected in mammary gland lavages collected from 10 clinically healthy virgin heifers before and after intramammary stimulation with synthetic muramyl dipeptide analogue. Using two-colour flow cytometry, CD2+ and CD2- subsets of γδ-TCR lymphocytes were analyzed. CD2+ γδ-TCR lymphocytes markedly prevailed over CD2- cells in intact mammary gland: 88.9 ± 4.9% of γδ-TCR lymphocytes were CD2+. After stimulation, neutrophils and γδ-TCR lymphocytes were recruited into the mammary gland. Among γδ-TCR lymphocytes, CD2- cells were mainly responsible for their expansion. After stimulation, 60.8 ± 13.4% of γδ-TCR lymphocytes were CD2+ (P < 0.01 when compared with mammary gland lavages before stimulation). It follows from the present study that the cells seem to be involved in the first phase of a response to an infection affecting mammary gland.

Keywords: cattle; udder; mastitis

Published: May 31, 2006  Show citation

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Faldyna M, Leva L, Sladek Z, Rysanek D, Toman M. γδ-TCR+ CD2- lymphocytes are recruited into bovine mammary gland after stimulation. Vet Med - Czech. 2006;51(5):258-264. doi: 10.17221/5545-VETMED.
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    References

    1. Asai K., Kai K., Rikiishi H., Sugawara S., Maruyama Y., Yamaguchi T., Ohta M., Kumagai K. (1998): Variation in CD4+ T and CD8+ T lymphocyte subpopulations in bovine mammary gland secretions during lactating and non-lactating periods. Veterinary Immunology and Immunopathology, 65, 51-61. Go to original source... Go to PubMed...
    2. Asai K., Komine Y., Kozutsumi T., Yamaguchi T., Komine K., Kumagai K. (2000): Predominat subpopulations of T lymphocytes in the mammary gland secretions during lactation and intraepithelial T lymphocytes in the intestine of dairy cows. Veterinary Immunology and Immunopathology, 73, 233-240. Go to original source... Go to PubMed...
    3. Bembridge G.P., MacHugh N.D., McKeever D., Awino E., Sopp P., Collins R.A., Gelder K.I., Howard C.J. (1995): CD45RO expression on bovine T cells: relation to biological function. Immunology, 86, 537-544. Go to PubMed...
    4. Boismenu R., Havran W.L. (1997): An innate view of γδ T cells. Current Opinions in Immunology, 9, 57-63. Go to original source... Go to PubMed...
    5. Clevers H., MacHugh N.D., Bansaid A., Dunlap S., Baldwin C.L., Kaushal A., Iams K., Howard C.J., Morrison W.I. (1990): Identification of a bovine surface antigen uniquely expressed on CD4-CD8-T cell receptor gamma/delta+ T lymphocytes. European Journal of Immunology, 20, 809-817. Go to original source... Go to PubMed...
    6. Collins R.A., Werling D., Duggan S.E., Bland A.P., Parson K.R., Howard C.J. (1998): γδ T cells present antigen to CD4+ αβ T cells. Journal of Leukococyte Biology, 63, 707-714. Go to original source... Go to PubMed...
    7. Concha C., Holmberg O., Astrom G. (1986): Cells found in non-infected and Staphylococcus-infected bovine mammary quarters and their ability to phagocytose fluorescent microspheres. Journal of Veterinary Medicine B, 33, 371-378. Go to original source... Go to PubMed...
    8. Crocker G., Sopp P., Parsons K., Davis W.C., Howard C.J. (1993): Analysis of the γδ T cell restricted antigen WC1. Veterinary Immunology and Immunopathology, 39, 137-144. Go to original source... Go to PubMed...
    9. Davis W.C., Splitter G.S. (1991): Individual antigens of cattle. Bovine CD2 (BoCD2). Veterinary Immunology and Immunopathology, 27, 43-50. Go to original source... Go to PubMed...
    10. Davis W.C., Brown W.C., Hamilton M.J., Wyatt C.R., Orden J.A., Khalid A.M., Naessens J. (1996): Analysis of monoclonal antibodies specific for the gamma delta TCR. Veterinary Immunology and Immunopathology, 52, 275-283. Go to original source... Go to PubMed...
    11. Ebling T.L., Fox L.K., Bayles K.W., Bohach G.A., Byrne K.M., Davis W.C., Ferens W.A., Hillers J.K. (2001): Bovine mammary immune response to an experimental intramammary infection with a Staphylococcus aureus strain containing a gene for staphylococcal enterotoxin C1. Journal of Dairy Science, 84, 2044- 2050. Go to original source... Go to PubMed...
    12. Fujihashi K., Mcghee J.R., Kweon M.N., Cooper M.D., Tonegawa S., Takahashi I., Hiroi T. Mestecky J., Kiyono H. (1996): γδ T cell deficient mice have impaired mucosal immunoglobulin A response. Journal of Experimental Medicine, 183, 1929-1935. Go to original source... Go to PubMed...
    13. Hayday A.C. (2000): γδ cells: A right time and a right place for a conserved third way of protection. Annual Review of Immunology, 18, 975-1026. Go to original source... Go to PubMed...
    14. Hein W.R., Mackay C.R. (1991): Prominence of γδ T cells in the ruminant immune system. Immunology Today, 12, 30-33. Go to original source... Go to PubMed...
    15. Inchaisri C., Persson-Waller K., Johannisson A. (2000): Studies on the modulation of leucocyte subpopulations and immunoglobulins following intramammary infusion of β1,3-glucan into the bovine udder during the dry period. Journal of Veterinary Medicine B, 47, 373-386. Go to original source... Go to PubMed...
    16. Jungi T.W., Sager H., Adler H., Brcic M., Pfister H. (1997): Serum factors, cell membrane CD14, and beta2 integrins are not required for activation of bovine macrophages by lipopolysaccharide. Infection and Immunity, 65, 3577-3584. Go to original source... Go to PubMed...
    17. Leitner G. Chaffer M., Krifucks O., Glickman A., Ezra E., Saran A. (2000a): Milk leucocyte populations in heifers free of udder infection. Journal of Veterinary Medicine B, 47, 133-138. Go to original source... Go to PubMed...
    18. Leitner G., Shoshani E., Krifucks O., Chaffer M., Saran A. (2000b): Milk leucocyte population patterns in bovine udder infection of different aetiology. Journal of Veterinary Medicine B, 47, 581-589. Go to original source... Go to PubMed...
    19. MacHugh N.D., Wijngaard P.L.J., Clevers H.C., Davis W.C. (1993): Clustering of monoclonal antibodies recognizing different members of the WC1 gene family. Veterinary Immunology and Immunopathology, 39, 155-160. Go to original source... Go to PubMed...
    20. MacHugh N.D., Mburu J.K., Carol M.J., Wyatt C.R., Orden J.A., Davis W.C. (1997): Identification of two distinct subsets of bovine γδ T cells with unique cell surface phenotype and tissue distribution. Immunology, 92, 340-345. Go to original source... Go to PubMed...
    21. Mackay C.R., Hein W.R. (1989): A large proportion of bovine T cells express the gamma delta T cell receptor and show a distinct tissue distribution and surface phenotype. International Immunology, 1, 540-545. Go to original source... Go to PubMed...
    22. Miller R.H., Paape M.J., Peters R.R., Young M.D. (1990): Total and differential somatic cell counts and N-acetyl(beta;-D-glucosaminidase activity in mammary gland secretions during dry period. Journal of Dairy Science, 73, 1751-1755. Go to original source... Go to PubMed...
    23. Miller R.H., Paape M.J., Fulton L.A. (1991): Variation in milk somatic cells in heifers at first calving. Journal of Dairy Science, 74, 3782-3790. Go to original source... Go to PubMed...
    24. Morrison W.I., Davis W.C. (1991): Differentiation antigens expressed predominantly on CD4- CD8- T lymphocytes (WC1, WC2). Veterinary Immunology and Immunopathology, 27, 71-76. Go to original source... Go to PubMed...
    25. Park Y.H., Fox L.K., Hamilton M.J., Davis W.C. (1992): Bovine mononuclear leukocyte subpopulations in peripheral blood and mammary gland secretions during lactation. Journal of Dairy Science, 75, 998-1006. Go to original source... Go to PubMed...
    26. Park Y.H., Jung S.C., Moon J.S., Ku B.G., Wyatt C.R., Hamilton M.J., Fox L.K., Davis W.C. (1994): A subset of mammary gland γδ T lymphocytes downregulates BoCD4 lymphocyte response to Staphylocccocus aureus in cattle with intramammary infection. Korean Journal of Immunology, 16, 19-27.
    27. Parsons K.R., Crocker G., Sopp P., Howard C.J., Davis W.C. (1993): Identification of monoclonal antibodies specific for the gamma/delta TCR. Veterinary Immunology and Immunopathology, 39, 161-167. Go to original source... Go to PubMed...
    28. Rebelatto M.C., Mead C., HogenEsch H. (2000): Lymphocyte populations and adhesion molecule expression in bovine tonsils. Veterinary Immunology and Immunopathology, 73, 15-29. Go to original source... Go to PubMed...
    29. Riollet C., Rainard P., Poutrel B. (2001): Cell subpopulations and cytokine expression in cow milk in response to chronic Stahylococcus aureus infection. Journal of Dairy Science, 84, 1077-1084. Go to original source... Go to PubMed...
    30. Rivas A.L., Quimby F.W., Coksaygan O., Alba A., Arina A., Arrobas M.J., Gonzalez R.N., Mohammed H.O., Lein D.H. (2001): Expression of CD3 and CD11b antigens on blood and mammary gland leukocytes and bacterial survival in milk of cows with experimentally induced Staphylococcus aureus mastitis. Americal Journal of Veterinary Research, 62, 1840-1851. Go to original source... Go to PubMed...
    31. Rysanek D., Babak V., Sladek Z., Toman M. (2001): Variations among unbred heifers in the activities of polymorphonuclear leucocytes from the mammary gland and blood. Journal of Veterinary Medicine B, 48, 31-41. Go to original source...
    32. Rysanek D., Sladek Z., Vasickova D., Faldyna M. (2005): Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation. Physiological Research, 54, 305-312. Go to original source...
    33. Sladek Z., Rysanek D. (2000): Apoptosis of polymorphonuclear leukocytes of the juvenile bovine mammary gland during induced influx. Veterinary Research, 31, 553-563. Go to original source... Go to PubMed...
    34. Sladek Z., Rysanek D. (2001a): Neutrophil apoptosis during the resolution of bovine mammary gland injury. Research in Veterinary Science, 70, 41-46. Go to original source... Go to PubMed...
    35. Sladek Z. Rysanek D. (2001b): Occurrence of aberrant secretion in bovine virgin mammary glands and effect on phagocytic activities of neutrophils. Czech Journal of Animal Science, 46, 377-382
    36. Sladek Z., Rysanek D. (2005): Tissue pool neutrophils of the bovine mammary gland: ultrastructural features during in vitro senescence. Anatomia, Histologia and Embryologica, 34, 159-166. Go to original source... Go to PubMed...
    37. Sladek Z., Rysanek D., Faldyna M. (2002): Activation of phagocytes during initiation and resolution of mammary gland injury induced by lipopolysaccharide. Veterinary Research, 33, 191-204. Go to original source... Go to PubMed...
    38. Sladek Z., Rysanek D., Ryznarova H., Faldyna M. (2005): Neutrophil apoptosis during experimentally induced Staphylococcus aureus mastitis. Veterinary Research, 36, 629-643. Go to original source... Go to PubMed...
    39. Soltys J., Quinn M.T. (1999): Selective recruitment of T-cell subsets to the udder during staphylococcal and streptococcal mastitis: Analysis of lymphocyte subsets and adhesion molecule expression. Infection and Immunity, 67, 6293-6302. Go to original source... Go to PubMed...
    40. Van Kampen C., Mallard B.A., Wilkie B.N. (1999): Adhesion molecules and lymphocyte subsets in milk and blood of periparturient Holstein cows. Veterinary Immunology and Immunopathology, 69, 23-32. Go to original source... Go to PubMed...
    41. Waters W.R., Harp J.A., Nonnecke B.J. (1995): Phenotypic analysis of peripheral blood lymphocytes and intestinal intra-epithelial lymphocytes in calves. Veterinary Immunology and Immunopathology, 48, 249-259. Go to original source... Go to PubMed...
    42. Wijngaard P.L.J., Metzelaar M.J., MacHugh N.D., Morrison W.I., Clevers H.C. (1992): Molecular characterization of the WC1 antigen expressed specifically on bovine CD4-CD8-T lymphocytes. Journal of Immunology, 149, 3273-3277. Go to original source... Go to PubMed...
    43. Wijngaard P.L.J., MacHugh N.D., Metzelaar M.J., Romberg S., Bansaid A., Davis W.C., Clevers H.C. (1994): Members of the novel WC1 gene family are differentially expressed on subsets of bovine CD4-CD8-γδ T lymphocytes. Journal of Immunology, 152, 3476-3482. Go to original source... Go to PubMed...
    44. Wilson E., Walcheck B., Davis W.C., Jutila M.A. (1998): Preferential tissue localization of bovine gamma delta T cell subsets defined by anti-T cell receptor for antigen antibodies. Immunology Letters, 64, 39-44. Go to original source... Go to PubMed...
    45. Wilson E., Hedges J.F., Butcher E.C., Briskin M., Jutila M.A. (2002): Bovine γδ T cell subsets express distinct patterns of chemokine responsiveness and adhesion molecules: A mechanism for tissue-specific γδ T cell subset accumulation. Journal of Immunology, 169, 4970-4975. Go to original source... Go to PubMed...
    46. Wyatt C.R., Madruga C., Cluff C., Parish S., Hamilton M.J., Goff W., Davis W.C. (1994): Differential distribution of γδ T-cell receptor lymphocyte subpopulations in blood and spleen of young and adult cattle. Veterinary Immunology and Immunopathology, 40, 187-199. Go to original source... Go to PubMed...
    47. Wyatt C.R., Bracket E.J., Perryman L.E., Davis W.C. (1996): Identification of γδ T lymphocyte subsets that populate calf ileal mucosa after birth. Veterinary Immunology and Immunopathology, 52, 91-103. Go to original source... Go to PubMed...
    48. Yamaguchi T., Hiratsuka M., Asai K., Kai K., Kumagai K. (1999): Differential distribution of T lymphocyte subpopulations in the bovine mammary gland during lactation. Journal of Dairy Science, 82, 1459-1464. Go to original source... Go to PubMed...

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