Vet Med - Czech, 2007, 52(10):445-450 | DOI: 10.17221/2045-VETMED

Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay

V.B. Kandimalla, N. Kandimalla, K. Hruska, M. Franek
Veterinary Research Institute, Brno, Czech Republic

During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. Hence, cost effective and simple screening methods are required for residual analysis of environmental and food samples on-site. Here, a simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase tracer conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 µg/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick.

Keywords: antibody; sulfonamides; residues

Published: October 31, 2007  Show citation

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Kandimalla VB, Kandimalla N, Hruska K, Franek M. Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay. Vet Med - Czech. 2007;52(10):445-450. doi: 10.17221/2045-VETMED.
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    References

    1. Botsoglou N.A., Fletouris D.J. (2001): Drug Residues in Foods: Pharmacology, Food Safety and Analysis. Macel Dekker, New York. 1194 pp.
    2. Campbell K., Fodey T., Flint J., Danks C., Danaher M., O'Keeffe M., Kennedy D.G., Elliott C. (2007): Development and validation of a lateral flow device for the detection of Nicarbazin contamination in poultry. Journal of Agricultural and Food Chemistry, 55, 2497-2503. Go to original source... Go to PubMed...
    3. Coung N.V., Bachmann T.T., Schmid R.D. (1999): Development of a dipstick immunoassay for quantitiative determination of 2,4-dichlorophenoxyacetic acid in water, fruit and urine samples. Fresenius Journal of Analytical Chemistry, 364, 584-589. Go to original source...
    4. Delmulle B.S., De Saeger M.D.G., Sibanda L., Vetero I. B., Peteghem C.H.V. (2005): Development of an immunoassay-based lateral flow dipstick for the rapid detection of aflatoxin B1 in pig feed. Journal of Agricultural and Food Chemistry, 53, 3365-3368. Go to original source... Go to PubMed...
    5. Diblikova I., Cooper K.M., Kennedy D.G., Franek M. (2005): Monoclonal antibody-based ELISA for the quantification of nitrofuran metabolite 3-amino-2oxazolidinone in tissues using a simplified sample preparation. Analytica Chimica Acta, 540, 285-292. Go to original source...
    6. EU Regulation 508/1999: Official Journal of the European Communities, 9.3. 1999, L60/16, 16-52.
    7. Franek M., Kolar V., Deng A., Crooks S. (1999): Determination of sulfadimidine (sulfamethazine) residues in milk, plasma, urine and edible tissues by sensitive ELISA. Food and Agricultural Immunology, 11, 339-349. Go to original source...
    8. Franek M., Diblikova I., Cernoch I., Vass M., Hruska K. (2006): Broad specificity immunoassays for sulfonamide detection: Immunochemical strategy for generic antibodies and competitors. Analytical Chemistry, 78, 1559-1567. Go to original source... Go to PubMed...
    9. Haasnoot W., Bienenmann-Ploum M., Lamminkaki U. (2005): Application of a multi-sulfonamide biosensor immunoassay for the detection of sulfadiazine and sulfamethoxazole residues in broiler serum and its use as a predictor of the levels in edible tissue. Analytica Chimica Acta, 552, 87-95. Go to original source...
    10. Kandimalla V.B., Tripathi V.S., Ju H.X. (2006): Conductive ormosil encapsulated with ferrocene conjugate and multiwall carbon nanotubes for biosensing application. Biomaterials, 27, 1167-1174. Go to original source... Go to PubMed...
    11. McGrath T., Baxter A., Ferguson J., Haughey S., Bjurling P. (2005): Multi sulfonamide screening in porcine muscle using a surface plasmon resonance biosensor. Analytica Chimica Acta, 529, 123-127. Go to original source...
    12. Novarro N.P., Bover C.G., Maquieira A., Puchades R. (2004): Specific polyclonal-based immunoassays for sulfathiazole. Analytical and Bioanalytical Chemistry, 379, 1088-1099. Go to original source... Go to PubMed...
    13. Okawa Y., Nagano M., Hirota S., Kobayashi H., Ohno T., Watanabe M. (1999): Tethered mediator biosensor. Mediated electron transfer between redox enzyme and electrode via ferrocene anchored to electrode surface with long poly(oxyethylene) chain. Biosensors and Bioelectronics, 14, 229-235. Go to original source...
    14. O'Keeffe M., Crabbe P., Salden M., Wichers J., Peteghem C.V., Kohen F., Pieraccini G., Noneti G.J. (2003): Preliminary evaluation of a lateral flow immunoassay device for the screening urine samples for the presence of sulphamethazine. Journal of Immunological Methods, 278, 117-126. Go to original source... Go to PubMed...
    15. Pal A., Acharya D., Saha D., Dhar T.K. (2004): Development of a membrane - based immunofiltration assay for the detection of T-2 toxin. Analytical Chemistry, 76, 4237-4240. Go to original source... Go to PubMed...
    16. Peruski A.H., Peruski L.F. (2003): Immunological methods for detection and identification of infectious disease and biological warfare agents. Clinical and Diagnostic Laboratory Immunology, 10, 506-513. Go to original source... Go to PubMed...

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