Veterinární medicína, 2007 (vol. 52), issue 10
The interrelationship between quality parameters of sperm before and after separation by gradient centrifugation
Z. Veznik, D. Svecova, A. Zajicova, Z. Reckova, J. Rubes
Vet Med - Czech, 2007, 52(10):423-429 | DOI: 10.17221/2051-VETMED 
Significance of a short-time survival test for the prediction of quality parameters of sperm obtained by gradient centrifugation was evaluated from an aspect of their functional resistance. Frozen semen samples from 27 bulls before and after separation were assessed by a 120-min survival test. The evaluation of morphological examination of ejaculates was carried out using the SASMO computer program. Statistically significant interrelationship between sperm quality parameters in the initial insemination doses, the amounts of obtained sperm after separation and their functional and vital resistance in the survival test was documented.
Genetic variability of Mycobacterium avium subsp. avium of pig isolates
M. Moravkova, M. Bartos, L. Dvorska-Bartosova, V. Beran, I. Parmova, M. Ocepek, M. Pate, I. Pavlik
Vet Med - Czech, 2007, 52(10):430-436 | DOI: 10.17221/2048-VETMED
The genetic diversity of 132 pig isolates of Mycobacterium avium subsp. avium (MAA) from the Czech Republic, the Slovak Republic and Slovenia was examined by IS901 restriction fragment length polymorphism (RFLP) analysis with restriction endonuclease PvuII. A total of 18 RFLP types were detected. The occurrence frequency of respective RFLP types varied between respective pig farms, with the exception of one RFLP type F found in 21 (34.4%) of 61 farms and in 10 (55.6%) of 18 farms in the Czech Republic and Slovak Republic, respectively. Two different RFLP types were detected in 5 (33.3%) of the 15 studied farms, from which...
Lactulose: effect on apoptotic- and immunological-markers in the gastro-intestinal tract of pre-ruminant calves
S. Fleige, W. PREIßINGER, H.H.D. Meyer, M.W. Pfaffl
Vet Med - Czech, 2007, 52(10):437-444 | DOI: 10.17221/2046-VETMED
The study was conducted to elucidate the effects of orally administered lactulose in combination with Enterococcus faecium on immune response of the intestinal tract in pre-ruminant calves. The mRNA expression of pro- and anti-inflammatory cytokines and proliferation and apoptosis markers were investigated in jejunum, ileum, colon and caecum. Simmental calves were fed diets containing 1% (L1) or 3% (L3) lactulose and the probiotic strain of the genus E. faecium, and compared with a non treated control group. Primarily the high dose feeding with lactulose showed an effect on several mRNA gene expression parameters. In the jejunum a down-regulation...
Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay
V.B. Kandimalla, N. Kandimalla, K. Hruska, M. Franek
Vet Med - Czech, 2007, 52(10):445-450 | DOI: 10.17221/2045-VETMED
During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. Hence, cost effective and simple screening methods are required for residual analysis of environmental and food samples on-site. Here, a simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase tracer conjugate, blocking agents and incubation times. Test results assessed by visual measurement...
European brown hare as a potential source of zoonotic agents
F. Treml, J. Pikula, H. Bandouchova, J. Horakova
Vet Med - Czech, 2007, 52(10):451-456 | DOI: 10.17221/2050-VETMED
There has recently been a growing interest in checking the state of health of European brown hares in hunting grounds because they are a susceptible bio-indicator of environmental changes and because of the dramatic decline in populations across Europe. A total of 1 051 (384, 302, and 365, respectively) blood sera were collected from hares during autumn hunting events and examined for tularaemia, brucellosis and leptospirosis in 2004, 2005 and 2006. Antibodies were found in 6.5, 1.6 and 7.5% of cases, respectively. Antibodies against Francisella tularensis, Brucella suis and leptospires were found in 7.9, 1.7 and 7.7% of females and 4.5,...
Spatiotemporal variation of some metal concentrations in oysters from the Mali Ston Bay, south-eastern Adriatic, Croatia - potential safety hazard aspect
A. Gavrilovic, E. Srebocan, J. Pompe-Gotal, Z. Petrinec, A. Prevendar-Crnic, Z. Matasin
Vet Med - Czech, 2007, 52(10):457-463 | DOI: 10.17221/2047-VETMED
The concentrations of cadmium, lead and zinc were determined in the soft tissue of oysters (Ostrea edulis L.) from three locations in the Mali Ston Bay on the south-eastern coast of the Adriatic Sea. The sampling was performed during two periods of breeding cycle, in summer (age ~1 year) and winter (age ~20 months, consumption size). The concentrations of cadmium, lead and zinc measured in the oyster soft tissue showed considerable spatiotemporal variations that could be attributed to seasonal differences in the freshwater inflow which varied between the study locations. Median concentrations measured at different locations and in different...
Fungal flora in the trachea of birds from a wildlife rehabilitation centre in Spain
M.E. Garcia, P. Lanzarot, V.L. Rodas, E. Costas, J.L. Blanco
Vet Med - Czech, 2007, 52(10):464-470 | DOI: 10.17221/2049-VETMED
In the present work we study the prevalence of fungal flora in the tracheal epithelium of wild birds in a rehabilitation centre. Two hundred and sixteen birds representing 26 species from seven orders were sampled. Yeasts and moulds were isolated from 92 of the birds sampled (42.5%); in 24.5% only yeasts, in 12.5% only moulds and in 5.5% both moulds and yeasts together. The cattle egret was where the greatest number of animals with positive isolation was detected. The prevalence of yeasts shows significant differences between raptors and Ciconiiformes. Within the yeast flora, basically there were strains belonging to the genus Candida, mainly...