Vet Med - Czech, 2007, 52(10):430-436 | DOI: 10.17221/2048-VETMED

Genetic variability of Mycobacterium avium subsp. avium of pig isolates

M. Moravkova1, M. Bartos1,2, L. Dvorska-Bartosova1, V. Beran1, I. Parmova3, M. Ocepek4, M. Pate4, I. Pavlik1
1 Veterinary Research Institute, Brno, Czech Republic
2 Veterinary and Pharmaceutical University, Brno, Czech Republic
3 State Veterinary Institute, Prague, Czech Republic
4 Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia

The genetic diversity of 132 pig isolates of Mycobacterium avium subsp. avium (MAA) from the Czech Republic, the Slovak Republic and Slovenia was examined by IS901 restriction fragment length polymorphism (RFLP) analysis with restriction endonuclease PvuII. A total of 18 RFLP types were detected. The occurrence frequency of respective RFLP types varied between respective pig farms, with the exception of one RFLP type F found in 21 (34.4%) of 61 farms and in 10 (55.6%) of 18 farms in the Czech Republic and Slovak Republic, respectively. Two different RFLP types were detected in 5 (33.3%) of the 15 studied farms, from which more than one isolate were examined. These results show the low variability of the MAA isolates among the pig farms and the possibility of various sources of infection for pigs from infected farms.

Keywords: avian tuberculosis; food safety; molecular epidemiology; mycobacteriosis; zoonosis

Published: October 31, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Moravkova M, Bartos M, Dvorska-Bartosova L, Beran V, Parmova I, Ocepek M, et al.. Genetic variability of Mycobacterium avium subsp. avium of pig isolates. Vet Med - Czech. 2007;52(10):430-436. doi: 10.17221/2048-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bartos M., Hlozek P., Svastova P., Dvorska L., Bull T., Matlova L., Parmova I., Kuhn I., Stubbs J., Moravkova M., Kintr J., Beran V., Melicharek I., Ocepek M., Pavlik I. (2006): Identification of members of Mycobacterium avium species by Accu-Probes, serotyping, and single IS900, IS901, IS1245 and IS901-flanking region PCR with internal standards. Journal of Microbiological Methods, 64, 333-345. Go to original source... Go to PubMed...
    2. Bono M., Jemmi T., Bernasconi C., Burki D., Telenti A., Bodmer T. (1995): Genotypic characterization of Mycobacterium avium strains recovered from animals and their comparison to human strains. Applied and Environmental Microbiology, 61, 371-373. Go to original source... Go to PubMed...
    3. Dvorska L., Bull T.J., Bartos M., Matlova L., Svastova P., Weston R.T., Kintr J., Parmova I., van Soolingen D., Pavlik I. (2003): A standardised restriction fragment length polymorphism (RFLP) method for typing Mycobacterium avium isolates links IS901 with virulence for birds. Journal of Microbiological Methods, 55, 11-27. Go to original source... Go to PubMed...
    4. Dvorska L., Matlova L., Bartos M., Parmova I., Bartl J., Svastova P., Bull T.J., Pavlik I. (2004): Study of Mycobacterium avium complex strains isolated from cattle in the Czech Republic between 1996 and 2000. Veterinary Microbiology, 99, 239-250. Go to original source... Go to PubMed...
    5. Dvorska L., Matlova L., Ayele W.Y., Fischer O.A., Amemori T., Weston R.T., Alvarez J., Beran V., Moravkova M., Pavlik I. (2007): Avian tuberculosis in naturally infected captive water birds of the Ardeideae and Threskiornithidae families studied by serotyping, IS901 RFLP typing, and virulence for poultry. Veterinary Microbiology, 119, 366-374. Go to original source... Go to PubMed...
    6. Glawischnig W., Steineck T., Spergser J. (2006): Infections caused by Mycobacterium avium subspecies avium, hominissuis, and paratuberculosis in free-ranging red deer (Cervus elaphus hippelaphus) in Austria, 2001- 2004. Journal of Wildlife Disease, 42, 724-731. Go to original source... Go to PubMed...
    7. Komijn R.E., de Haas P.E.W., Schneider M.M.E., Eger T., Nieuwenhuijs J.H.M., van den Hoek R.J., Bakker D., Erveld F.G.V., van Soolingen D. (1999): Prevalence of Mycobacterium avium in slaughter pigs in The Netherlands and comparison of IS1245 restriction fragment length polymorphism patterns of porcine and human isolates. Journal of Clinical Microbiology, 37, 1254- 1259. Go to original source... Go to PubMed...
    8. Kunze Z.M., Portaels F., McFadden J.J. (1992): Biologically distinct subtypes of Mycobacterium avium differ in possession of insertion sequence IS901. Journal of Clinical Microbiology, 30, 2366-2372. Go to original source... Go to PubMed...
    9. Matlova L., Dvorska L., Palecek K., Maurenc L., Bartos M., Pavlik I. (2004): Impact of sawdust and wood shavings in bedding on pig tuberculous lesions in lymph nodes, and IS1245 RFLP analysis of Mycobacterium avium subsp. hominissuis of serotypes 6 and 8 isolated from pigs and environment. Veterinary Microbiology, 102, 227-236. Go to original source... Go to PubMed...
    10. Matlova L., Dvorska L., Ayele W.Y., Bartos M., Amemori T., Pavlik I. (2005): Distribution of Mycobacterium avium complex isolates in tissue samples of pigs fed peat naturally contaminated with mycobacteria as a supplement. Journal of Clinical Microbiology, 43, 1261-1268. Go to original source... Go to PubMed...
    11. Mijs W., de Haas P., Rossau R., van der Laan T., Rigouts L., Portaels F., van Soolingen D. (2002): Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium to bird-type isolates and M. avium subsp. hominissuis for the human/porcine type of M. avium. International Journal of Systematic and Evolutionary Microbiology, 52, 1505-1518. Go to original source... Go to PubMed...
    12. Mobius P., Lentzsch P., Moser I., Naumann L., Martin G., Kohler H. (2006): Comparative macrorestriction and RFLP analysis of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. hominissuis isolates from man, pig, and cattle. Veterinary Microbiology, 117, 284-291. Go to original source... Go to PubMed...
    13. Ocepek M., Pate M., Zolnir-Dovc M., Cvetnic Z. (2003): Tuberculosis in cattle caused by IS901+ Mycobacterium avium subsp. avium: a case report. Veterinarni Medicina, 48, 47-50. http://www.vri.cz/docs/vetmed/48-2-47.pdf Go to original source...
    14. Offermann U., Bodmer T., Audige L., Jemmi T. (1999): The prevalence of Salmonella, Yersinia and mycobacteria in slaughtered pigs in Switzerland. Schweizer Archiv fur Tierheilkunde, 141, 509-515. Go to PubMed...
    15. Pate M., Ocepek M., Zolnir-Dovc M., Krt B. (2005): Characterization of genetic diversity of animal and human Mycobacterium avium strains by IS1245- IS1311 spacer typing. Veterinarni Medicina, 50, 175- 180. http://www.vri.cz/docs/vetmed/50-4-175.pdf Go to original source...
    16. Pavlik I., Svastova P., Bartl J., Dvorska L., Rychlik I. (2000): Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals, humans, and the environment and virulence for poultry. Clinical and Diagnostic Laboratory Immunology, 7, 212-217. Go to original source... Go to PubMed...
    17. Pavlik I., Matlova L., Dvorska L., Bartl J., Oktabcova L., Docekal J., Parmova I. (2003): Tuberculous lesions in pigs in the Czech Republic during 1990-1999: occurrence, causal factors and economic losses. Veterinarni Medicina, 48, 113-125. http://www.vri.cz/docs/vetmed/48-5-113.pdf Go to original source...
    18. Pavlik I., Matlova L., Dvorska L., Shitaye J.E., Parmova I. (2005): Mycobacterial infections in cattle and pigs caused by Mycobacterium avium complex members and atypical mycobacteria in the Czech Republic during 2000-2004. Veterinarni Medicina, 50, 281-290. http://www.vri.cz/docs/vetmed/50-7-281.pdf Go to original source...
    19. Pillai S.R., Jayarao B.M., Gummo J.D., Hue E.C., Tiwari D., Stabel J.R., Whitlock R.H. (2001): Identification and sub-typing of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium by randomly amplified polymorphic DNA. Veterinary Microbiology, 79, 275-284. Go to original source... Go to PubMed...
    20. Ritacco V., Kremer K., van der Laan T., Pijnenburg J.E.M, de Haas P.E., van Soolingen D. (1998): Use of IS901 and IS1245 in RFLP typing of Mycobacterium avium complex: relatedness among serovar reference strains, human and animal isolates. International Journal of Tuberculosis and Lung Disease, 2, 242-251.
    21. Romano M.I., Amadio A., Bigi F., Klepp L., Etchechoury I., Llana M.N., Morsella C., Paolicchi F., Pavlik I., Bartos M., Leao S.C., Cataldi A. (2005): Further analysis of VNTR and MIRU in the genome of Mycobacterium avium complex, and application to molecular epidemiology of isolates from South America. Veterinary Microbiology, 110, 221-237. Go to original source... Go to PubMed...
    22. Shitaye J.E., Parmova I., Matlova L., Dvorska L., Horvathova A., Vrbas V., Pavlik I. (2006): Mycobacterial and Rhodococcus equi infections in pigs in the Czech Republic between the years 1996 and 2004: the causal factors and distribution of infections in the tissues. Veterinarni Medicina, 51, 497-511. http://www.vri.cz/docs/vetmed/51-11-497.pdf Go to original source...
    23. Thibault V.C., Grayon M., Boschiroli M.L., Hubbans C., Overduin P., Stevenson K., Gutierrez M.C., Supply P., Biet F. (2007): New variable number tandem repeat markers for typing M. avium subsp. paratuberculosis and M. avium strains: comparison with IS900 RFLP and IS1245 RFLP typing. Journal of Clinical Microbiology, 45, 2404-2410. Go to original source... Go to PubMed...
    24. Trckova M., Zraly Z., Matlova L., Beran V., Moravkova M., Svobodova J., Pavlik I. (2006a): Effects of peat feeding on the performance and health status of fattening pigs and environmentally derived mycobacteria. Veterinarni Medicina, 51, 533-543. http://www.vri.cz/docs/vetmed/51-12-533.pdf Go to original source...
    25. Trckova M., Zraly Z., Bejcek P., Matlova L., Beran V., Horvathova A., Faldyna M., Moravkova M., Shitaye J.E., Svobodova J., Pavlik, I. (2006b): Effect of feeding treated peat as a supplement to newborn piglets on the growth, health status and occurrence of conditionally pathogenic mycobacteria. Veterinarni Medicina, 51, 544-554. http://www.vri.cz/docs/vetmed/51-12-544.pdf Go to original source...
    26. van Soolingen D., Bauer J., Ritacco V., Leao S.C., Pavlik I., Vincent V., Rastogi N., Gori A., Bodmer T., Garzelli C., Garcia M.J. (1998): IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization. Journal of Clinical Microbiology, 36, 3051-3054. Go to original source... Go to PubMed...
    27. Wolinsky E., Schaefer W.B. (1973): Proposed numbering scheme for mycobacterial serotypes by agglutination. International Journal of Systematic Bacteriology, 23, 182-183. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content