Vet Med - Czech, 2008, 53(5):250-254 | DOI: 10.17221/1855-VETMED

Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism

A. Kozubska-Sobocinska1, B. Rejduch1,2
1 National Research Institute of Animal Production, Balice n. Krakow, Poland
2 Department of Biotechnology, University of Rzeszow, Kolbuszowa n. Rzeszow, Poland

The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.

Keywords: sheep; leukocyte chimerism; FISH; spermatozoa; heterosomes

Published: May 31, 2008  Show citation

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Kozubska-Sobocinska A, Rejduch B. Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism. Vet Med - Czech. 2008;53(5):250-254. doi: 10.17221/1855-VETMED.
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