Veterinární medicína, 2008 (vol. 53), issue 5
Babesiosis as a disease of people and dogs. Molecular diagnostics: a review
B. Skotarczak
Vet Med - Czech, 2008, 53(5):229-235 | DOI: 10.17221/1946-VETMED 
Babesia is the causative agent of babesiosis, a tick-borne zoonosis which has been increasingly described throughout the world. Babesia microti and Babesia divergens are the etiological agents of human babesiosis. Babesia canis is the principal etiological agent of canine babesiosis. Currently, the diagnostics of babesiosis is based mainly on serological methods and the immunofluorescent antibody test (IFA) is most commonly used. However, even in the acute phase of the disease, seroconversion does not always occur. Clinical symptoms, because of their unspecificity, cannot be used to make a correct diagnosis. In this situation...
Comparative inactivation of Aujeszky's disease virus, Porcine teschovirus and Vesicular stomatitis I by chemical disinfectants
H. Dvorakova, J. Prodelalova, M. Reichelova
Vet Med - Czech, 2008, 53(5):236-242 | DOI: 10.17221/1949-VETMED
We tested the germicide activity of 1% Chloramin BM, 1% Incidin Plus, 1% Lysoformin 3000, 0.2% Mikasept KP, and 2% Sekusept Forte against viruses in suspension (suspension test) and dried onto a surface (carrier test). The agents of the porcine encephalomyelitis (Porcine teschovirus, strains CAPM V-86, CAPM V-37), Aujeszky's disease (strains CAPM V-166, CAPM V-327) and vesicular stomatitis (strains CAPM V-499, CAPM V-331) were used as model viruses. After 30 min contact time in both the suspension and carrier tests, the Porcine teschovirus was 4 lg inactivated only by Mikasept, which was thus the only disinfectant to meet the standard....
Environmental influence in cyathostominae ecology
M.C.M. Couto, S. Quinelato, C.N. Santos, L.S. Souza, I.B.M. Sampaio, M.L.A. Rodrigues
Vet Med - Czech, 2008, 53(5):243-249 | DOI: 10.17221/1950-VETMED
Studies of the survival, recovery and migration of cyathostomin infective larvae in a Bermuda grass (Cynodon dactylon) pasture were carried out in the Baixada Fluminense county, Rio de Janeiro state. Fresh feces (± 1 kg) from naturally infected horses were deposited monthly on Bermuda grass. Samples of feces and surrounding grass were collected every seven days, from March 2005 to March 2007, and larva were counted. In the feces, cyathostomin L3 survived for up to 15 weeks, with higher recovery rates during the rainy period (46 228/kg dried herbage - dh), and on the grass for up to 12 weeks. The recovery of L3 was...
Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism
A. Kozubska-Sobocinska, B. Rejduch
Vet Med - Czech, 2008, 53(5):250-254 | DOI: 10.17221/1855-VETMED
The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No....
Changes in white blood cells in sheep blood during selenium supplementation
L. Pisek, J. Travnicek, J. Salat, V. Kroupova, M. Soch
Vet Med - Czech, 2008, 53(5):255-259 | DOI: 10.17221/1947-VETMED
The aim of the experiment was to evaluate the impact of selenium supplementation on white blood cell parameters in the blood of ewes. The total white blood cell (WBC) and differentiation of leukocytes in blood smear were detected by a microscopic analysis, and the CD4+ and CD8+ subsets were detected by flow cytometry. A decrease in the count of WBC was recorded during pregnancy; it was statistically significant only in the group supplemented with organic selenium. In the postpartal period there was a statistically significant increase in the percentages of CD4+ and CD8+ subsets but differences between the...
Dynamics of IgG in the blood serum of sheep with different selenium intake
H. Rodinova, V. Kroupova, J. Travnicek, M. Stankova, L. Pisek
Vet Med - Czech, 2008, 53(5):260-265 | DOI: 10.17221/1944-VETMED
An experiment was conducted to study the effect of supplementation of ewes with selenium in an inorganic and organic form on the level of IgG during infertility, pregnancy and lactation and in their offspring until two months of age. Control Group C (n = 5) was without Se supplementation. Experimental groups: E1 (n = 5) was supplemented with 180 μg Na2SeO3 per ewe/day, E2 (n = 5) received 180 μ Se per ewe/day organically bound in the biomass of an alga of the genus Chlorella. The average number of newborn lambs per lambing ewe was 1.0, 1.0 and 1.8 in Group C, E1 and E2, respectively. The supplementation...
Serologic survey of wild boars for mosquito-borne viruses in South Moravia (Czech Republic)
J. Halouzka, Z. Juricova, J. Jankova, Z. Hubalek
Vet Med - Czech, 2008, 53(5):266-271 | DOI: 10.17221/1945-VETMED
A serosurvey for mosquito-borne viruses was carried out in 93 wild boars (Sus scrofa), using a plaque-reduction neutralization microtest with Vero cells. The boars were sampled on 24 hunting grounds of the Breclav district (South Moravia) from 2000 to 2002. Specific antibodies to Flavivirus West Nile (WNV) were detected in six (6.5%) animals, and only in Lanzhot and Kostice, i.e., in the area of the "Soutok" game reserve where WNV was previously isolated from mosquitoes in South Moravia. However, the antibody titres were comparatively low (1:20-1:40). A substantially higher seroprevalence was revealed against Orthobunyavirus Tahyna...
The morphology of circulus arteriosus cerebri in the red squirrel (Sciurus vulgaris)
A. Aydin
Vet Med - Czech, 2008, 53(5):272-276 | DOI: 10.17221/1948-VETMED
In this study, the circulus arteriosus cerebri of the squirrel was investigated. Ten squirrel were used. Coloured latex was given from left ventriculi of the all squirrels. Circulus arteriosus cerebri was examined after the dissection was made. The basilar artery was formed by merge of the right and left vertebral artery. The caudal communicans artery which was caudal part of circulus arteriosus cerebri was formed by the basilar artery on sulcus pontocrurale. From caudal to cranial, the branches originated from the basilar artery and circulus arteriosus cerebri to cerebrum and cerebellum were as follows: the caudal cerebelli artery,rami ad pontem,...
Reconstruction of temporomandibular joint ankylosis with temporalis myofascial flap in a cat: a case report
S.Y. Heo, H.B. Lee, K.C Lee, N.S. Kim
Vet Med - Czech, 2008, 53(5):277-281 | DOI: 10.17221/1951-VETMED
An approximately 7-year-old 1.7kg, female Siamese cat with a history of a malocclusion and an inability to open its mouth was referred the Animal Medical Center, Chonbuk National University. The cat had shown signs since it was adopted four years earlier but the cause was not determined. The mandible was deviated 2 mm to left, the mouth could be opened only 2 mm and attempts to open the jaws caused considerable pain. Radiographs indicated new bone formation and a loss of joint space in the right temporomandibular joint(TMJ). 3-dimensional CT showed bony fusion and bone thickness in the right TMJ. The physical, 3-dimensional CT and radiographic examinations...