Vet Med - Czech, 2008, 53(5):255-259 | DOI: 10.17221/1947-VETMED

Changes in white blood cells in sheep blood during selenium supplementation

L. Pisek1, J. Travnicek1, J. Salat2, V. Kroupova1, M. Soch1
1 Faculty of Agriculture, University of South Bohemia, Ceske Budejovice, Czech Republic
2 Institute of Parasitology of the Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic

The aim of the experiment was to evaluate the impact of selenium supplementation on white blood cell parameters in the blood of ewes. The total white blood cell (WBC) and differentiation of leukocytes in blood smear were detected by a microscopic analysis, and the CD4+ and CD8+ subsets were detected by flow cytometry. A decrease in the count of WBC was recorded during pregnancy; it was statistically significant only in the group supplemented with organic selenium. In the postpartal period there was a statistically significant increase in the percentages of CD4+ and CD8+ subsets but differences between the groups were not statistically significant. The results of the experiment documented that the supplementation of different forms of selenium did not markedly influence the dynamics of blood parameters in non-pregnant, pregnant and lactating ewes if the intake of vitamins and other essential microelements was adequate.

Keywords: ewes; immunity; T lymphocytes; CD4+; CD8+

Published: May 31, 2008  Show citation

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Pisek L, Travnicek J, Salat J, Kroupova V, Soch M. Changes in white blood cells in sheep blood during selenium supplementation. Vet Med - Czech. 2008;53(5):255-259. doi: 10.17221/1947-VETMED.
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References

  1. Barrington G.M., Parish S.M. (2001): Bovine neonatal immunology. The Veterinary Clinics of North America. Food Animal Practice, 17, 463-476. Go to original source... Go to PubMed...
  2. Behne D., Kyriakopoulos A. (2001): Mammalian selenium-containing proteins. Annual Review of Nutrition, 21, 453-473. Go to original source... Go to PubMed...
  3. Entrican G., Buxton D., Longbottom D. (2002): Chlamydial infection in sheep: immune control versus fetal pathology. Journal of the Royal Society of Medicine, 94, 273-277. Go to original source... Go to PubMed...
  4. Fekete S.G., Kellems R.O. (2007): Interrelationship of feeding with immunity and parasitic infection: a review. Veterinarni Medicina, 52, 131-143. Go to original source...
  5. Kursa J. (1969): Nutritive degeneration of muscle in young cattle in Sumava region (in Czech). Veterinarni Medicina, 14, 549-553.
  6. Mackay C.R. (1993): Immunological memory. Advances in Immunology, 53, 217. Go to original source... Go to PubMed...
  7. Nasar A., Rahman A., Meeusen N.T., Lee C.S. (2002): Peri-partum changes in the intraepithelial lymphocyte population of sheep interplacentomal endometrium. American Journal of Reproductive Immunology, 47, 132-141. Go to original source... Go to PubMed...
  8. Naskalski J.W., Kusnierz-Cabala B., Kedra B., Dumnicka P., Panek J., Maziarz B. (2007): Correlation of peripheral blood monocyte and neutrophil direct counts with plasma inflammatory cytokines and TNF-alpha soluble receptors in the initial phase of acute pancreatitis. Advances in Medical Sciences, 52, 129-134.
  9. Park Y.H., Joo Y.S., Park J.Y., Moon J.S., Kim S.H., Kwon N.H., Ahn J.S., Davis W.C., Davies C.J. (2004): Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitisresistant and susceptible cows. Journal of Veterinary Science, 5, 29-39. Go to original source... Go to PubMed...
  10. Pavlata L., Pechova A., Becvar O., Illek J. (2001): Muscular dystrophy in dairy cows following a change in housing technology. Acta Veterinaria Brno, 70, 269-275. Go to original source...
  11. Reiterova K., Tomasovicova O., Dubinsky P. (2004): Postparturitional changes in the proportion of CD4+ and CD8+ T lymphocytes in Toxocara canis-infected mice and their offspring. Veterinarni Medicina, 49, 103-108. Go to original source...
  12. Rogers L.B., Kaack M.B., Henson M.C., Rasmussen T., Henson E., Veazey R.S., Krogstad D.J., Davison B.B. (2005): Hematologic and lymphocyte immunophenotypic reference values for normal rhesus monkey (Macaca mulatta) umbilical cord blood; gravidity may play a role in study design. Journal of Medical Primatology, 34, 147-153. Go to original source... Go to PubMed...
  13. Scott J.L., Ketheesan N., Summers P.M. (2006): Leucocyte population changes in the reproductive tract of the ewe in response to insemination. Reproduction, Fertility and Development, 18, 627-634. Go to original source... Go to PubMed...
  14. Smyth J.B., Wang J.H., Barlow R.M., Humpheys D.J., Robins M., Stodulski J.B. (1990): Experimental acute selenium intoxication in lambs. Journal of Comparative Pathology, 102, 197-209. Go to original source... Go to PubMed...
  15. Thorp B.H., Seneque S., Staute K., Kimpton W.G. (1991): Characterization and distribution of lymphocyte subsets in sheep hemal nodes. Developmental and Comparative Immunology, 15, 393-400. Go to original source... Go to PubMed...
  16. Travnicek J., Pisek L., Herzig I., Doucha J., Kvicala J., Kroupova V., Rodinova H. (2007): Selenium content in the blood serum and urine of ewes receiving selenium-enriched unicellular alga Chlorella. Veterinarni Medicina, 52, 42-48. Go to original source...
  17. Trnka Z., Cahill R.N.P. (1980): Aspects of the immune response in single lymph nodes. Monographs in Allergy, 16, 245-259. Go to PubMed...
  18. Whanger P.D. (2002): Selenocompounds in plants and animals and their biological significance. Journal of the American College of Nutrition, 21, 223-232. Go to original source... Go to PubMed...
  19. Wu L., Guo X., Banuelos G.S. (1997): Accumulation of seleno-amino acids in legume and grass plant species grown in selenium-laden soils. Environmental Toxicology and Chemistry, 16, 491-497. Go to original source...
  20. Yuben P.M., Dorasamyb T., Venketasamyb S., Naickerb V., Lallooa U.G. (2000): Correlation of CD4:CD8 ratio and tumour necrosis factor (TNF&alpha) levels in induced sputum with bronchoalveolar lavage fluid in pulmonary sarcoidosis. Thorax, 55, 696-699. Go to original source... Go to PubMed...

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