Vet Med - Czech, 2008, 53(5):266-271 | DOI: 10.17221/1945-VETMED

Serologic survey of wild boars for mosquito-borne viruses in South Moravia (Czech Republic)

J. Halouzka1, Z. Juricova1, J. Jankova1,2, Z. Hubalek1,2
1 Institute of Vertebrate Biology of the Academy of Sciences of the Czech Republic, Brno, Czech Republic
2 Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

A serosurvey for mosquito-borne viruses was carried out in 93 wild boars (Sus scrofa), using a plaque-reduction neutralization microtest with Vero cells. The boars were sampled on 24 hunting grounds of the Breclav district (South Moravia) from 2000 to 2002. Specific antibodies to Flavivirus West Nile (WNV) were detected in six (6.5%) animals, and only in Lanzhot and Kostice, i.e., in the area of the "Soutok" game reserve where WNV was previously isolated from mosquitoes in South Moravia. However, the antibody titres were comparatively low (1:20-1:40). A substantially higher seroprevalence was revealed against Orthobunyavirus Tahyna (TAHV): 18 (19.4%) wild boars were positive, and the titres ranged from 1:20 up to 1:640. Only one animal (1.1%) seroreacted with Orthobunyavirus Batai (Calovo), at a low titre of 1:20. The sera were additionally examined by a haemagglutination-inhibition test against Alphavirus Sindbis: two boars (2.2%) revealed antibodies, the titres were 1:20 and 1:80. The serosurvey indicates that the activity of mosquito-borne viruses in South Moravia has decreased compared with the past decades, but that surveillance for these viruses is still necessary.

Keywords: antibodies; West Nile virus; Tahyna virus; Batai virus; Sindbis virus; Czechland; swine

Published: May 31, 2008  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Halouzka J, Juricova Z, Jankova J, Hubalek Z. Serologic survey of wild boars for mosquito-borne viruses in South Moravia (Czech Republic). Vet Med - Czech. 2008;53(5):266-271. doi: 10.17221/1945-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aspock H., Kunz C. (1971). Antikorper gegen TahynaVirus und Calovo-Virus in wildlebenden und domestizierten Saugetieren im ostlichen Neusiedlersee-Gebiet (Ost-Osterreich). Zentralblatt fur Bakteriologie I Originale, 216, 435-440.
    2. Bakonyi T., Hubalek Z., Rudolf I., Nowotny N. (2005): Novel flavivirus or new lineage of West Nile virus, Central Europe. Emerging Infectious Diseases, 11, 225-231. Go to original source... Go to PubMed...
    3. Bardos V., Cupkova E. (1962): The Calovo virus - the second virus isolated from mosquitoes in Czechoslovakia. Journal of Hygiene Epidemiology Microbiology and Immunlogy, 6, 186-192.
    4. Bardos V., Medek M., Kania V., Hubalek Z. (1975): Isolation of Tahyna virus from the blood of sick children. Acta Virologica, 19, 447. Go to PubMed...
    5. Calisher C.H., Karabatsos N., Dalrymple J.M., Shope R. E., Porterfield J.S., Westaway E.G., Brandt W.E. (1989): Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. Journal of General Virology, 70, 37-43. Go to original source... Go to PubMed...
    6. Clarke D.H., Casals J. (1958): Techniques for hemagglutination and hemagglutination-inhibition test with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene, 7, 561-573. Go to original source... Go to PubMed...
    7. Danielova V., Marhoul Z. (1968): Incidence of antibodies against some arboviruses in humans, domestic and wild animals living in the natural focus of Tahyna virus in South Moravia (in Czech). Ceskoslovenska Epidemiologie Mikrobiologie a Imunologie, 17, 155-161.
    8. Danielova V., Malkova D., Minar J., Ryba J. (1976): Dynamics of the natural focus of Tahyna virus in southern Moravia and species succession of its vectors, the mosquitoes of the genus Aedes. Folia Parasitologica, 23, 243-249. Go to PubMed...
    9. Ernek E., Kozuch O., Gresikova M., Nosek J., Sekeyova M. (1973): Isolation of Sindbis virus from the reed warbler (Acrocephalus scirpaceus) in Slovakia. Acta Virologica, 17, 359-361. Go to PubMed...
    10. Holden P., LaMotte L.C., Shriner R.B. (1965): Arbovirushemagglutinin inhibitor in acetone-extracted serums from normal chickens. Science, 147, 169-170. Go to original source... Go to PubMed...
    11. Hubalek Z., Chanas A.C., Johnson B.K., Simpson D.I.H. (1979): Cross-neutralization study of seven California group (Bunyaviridae) strains in homoiothermous (PS) and poikilothermous (XTC-2) vertebrate cells. Journal of General Virology, 42, 357-362. Go to original source... Go to PubMed...
    12. Hubalek Z., Halouzka J. (1996): Arthropod-borne viruses of vertebrates in Europe. Acta Scientiarum Naturalium Brno, 10, No. 4-5, 1-95.
    13. Hubalek Z., Halouzka J., Juricova. Z., Sebesta O. (1998): First isolation of mosquito-borne West Nile virus in the Czech Republic. Acta Virologica, 42, 119-120. Go to PubMed...
    14. Hubalek Z., Savage H.M., Halouzka J., Juricova Z., Sanogo Y.O., Lusk S. (2000): West Nile virus investigations in South Moravia, Czechland. Viral Immunology, 13, 427-433. Go to original source...
    15. Juricova Z. (1992): Antibodies to arboviruses in game animals in South Moravia (in Czech). Veterinarni Medicina, 37, 633-636.
    16. Juricova Z., Hubalek Z. (1999): Serological surveys for arboviruses in the game animals of southern Moravia (Czech Republic). Folia Zoologica, 48: 185-189.
    17. Kolman J.M. (1973): Serologic examination of some domestic animals from South Moravia on the presence of antibodies to selected arboviruses of the A, B, California and Bunyamwera groups. Folia Parasitologica, 20, 353-360. Go to PubMed...
    18. Kozuch O., Nosek J., Gresikova M., Ernek E. (1976): Surveillance of mosquito-borne natural focus in Zahorska Lowland, 115-118. In: Sixl W. (ed.): 2nd International Arbeitskolloquium uber die Naturherde von Infektionskrankheiten in Zentraleuropa. Hygiene Institut der Universitat, Graz.
    19. Labuda M., Kozuch O., Gresikova M. (1974): Isolation of West Nile virus from Aedes cantans mosquitoes in West Slovakia. Acta Virologica, 18, 429-433.
    20. Madrid A.T., Porterfield J.S. (1969): A simple micro-culture method for the study of group B arboviruses. Bulletin WHO, 40, 113-121.
    21. Madrid A.T., Porterfield J.S. (1974): The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology, 23, 91-96. Go to original source... Go to PubMed...
    22. Malkova D., Danielova V., Holubova J., Marhoul Z. (1986): Less known arboviruses in Central Europe (in Czech). Rozpravy CSAV, rada matem.-prirodnich ved, 96, No. 5, 1-75.
    23. Melnick J.L., Paul J.R., Riordan J.F., Barnett V.H.H., Goldblum N., Zabin E. (1951): Isolation from human sera in Egypt of a virus apparently identical to West Nile virus. Proceedings of the Society for Experimental Biology and Medicine, 77, 661-665. Go to original source... Go to PubMed...
    24. Niedrig M., Sonnenberg K., Steinhagen K., Paweska J.T. (2007): Comparison of ELISA and immunoassays for measurement of IgG and IgM antibody to West Nile virus in human sera against virus neutralisation. Journal of Virological Methods, 139, 103-105. Go to original source... Go to PubMed...
    25. Pospisil L., Jandasek L., Pesek J. (1954): Isolation of new strains of meningoencephalitis virus in the Brno area during summer of 1953 (in Czech). Lekarske Listy (Brno), 9, 3-5.
    26. Rosicky B., Malkova D., eds. (1980): Tahyna virus natural focus in southern Moravia (in Czech). Rozpravy CSAV, rada matem.-prirodnich ved, 90, 1-107.
    27. Smetana K., Danielova V., Kolman J.M., Malkova D., Minar J. (1967): The isolation of the Calovo virus from the mosquitoes of the group Anopheles maculipennis in southern Moravia. Journal of Hygiene Epidemiology Microbiology and Immunology, 11, 55-59. Go to PubMed...
    28. Taylor R.M., Hurlbut H.S., Work T.H., Kingston J.R., Frotingham T.E. (1955): Sindbis virus: a newly recognized arthropod-transmitted virus. American Journal of Tropical Medicine and Hygiene, 4, 844-862. Go to original source... Go to PubMed...
    29. Theiler M., Downs W.G. (1973): The Arthropod-Borne Viruses of Vertebrates. Yale University Press, New Haven and London. 578 pp.
    30. Weingartl H.M., Drebot M.A., Hubalek Z., Halouzka J., Andonova M., Dibernardo A., Cottam-Birt C., Larence J., Marszal P. (2003): Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Canadian Journal of Veterinary Research, 67, 128-132.
    31. Weissenboock H., Kolodziejek J., Url A., Lussy H., RebelBauder B., Nowotny N. (2002): Emergence of Usutu virus, an African mosquito-borne flavivirus of the Japanese encephalitis virus group, Central Europe. Emerging Infectious Diseases, 8, 652-656. Go to original source... Go to PubMed...
    32. Weissenboock H., Hubalek Z., Halouzka J., Pichlmair A., Maderner A., Fragner K., Kolodziejek J., Loupal G., Kolbl S., Nowotny N. (2003): Screening for West Nile virus infections in susceptible animal species in Austria. Epidemiology and Infection, 131, 1023- 1027. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content