Vet Med - Czech, 2009, 54(5):205-214 | DOI: 10.17221/58/2009-VETMED

Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells

M.K. Kowalik, D. Slonina, J. Kotwica
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

Progesterone (P2 (PGES), (b) protein expression of COX-2, PGFS and PGES, and (c) P4 receptor membrane component 1 (PGRMC1) gene expression in bovine endometrial cells. The epithelial endometrial cells (2.5 × 105/ml) from Days 14-16 of the oestrous cycle were incubated for 72-96 h to attach the cells to the bottom of a well. Next, the cells were preincubated for 30 min with P4 and P5 (10-5M each) and incubated for 4 h and 6 h alone or with OT (10-7M). Thereafter, the medium was collected for PGE2 and PGFM determination, while cells were harvested for gene and protein expression analysis. The used steroids: (a) inhibited OT-stimulated PGF2α, but not PGE2 secretion from endometrial cells, (b) did not affect the expression of mRNA for COX-2, PGFS, PGES and PGRMC1 in endometrial cells after 4 and 6 h, (c) they decreased OT-stimulated COX-2 mRNA expression only after 6 h incubation, and (d) did not influence COX-2, PGFS and PGES protein expression after 6 h. These results indicate that P4 and P5 inhibit OT-stimulated secretion/production of luteolytic PGF2α by a transcription-independent mechanism and partly by down-regulation of COX-2 mRNA.

Keywords: non-genomic effect; progesterone; progesterone membrane receptor component 1; endometrium; bovine

Published: May 31, 2009  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kowalik MK, Slonina D, Kotwica J. Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells. Vet Med - Czech. 2009;54(5):205-214. doi: 10.17221/58/2009-VETMED.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arosh J.A., Parent J., Chapdelain P., Sirois J., Fortier M.A. (2002): Expression of cyclooxygenases 1 and 2 and prostaglandin E synthase in bovine endometrial tissue during the oestrus cycle. Biology of Reproduction, 67, 161-169. Go to original source... Go to PubMed...
    2. Asselin E., Fortier M.A. (2000): Detection and regulation of the messenger for a putative bovine endometrial 9keto-prostaglandin E2 reductase: effect of oxytocin and interferon-tau. Biology of Reproduction, 62, 125-131. Go to original source... Go to PubMed...
    3. Asselin E., Drolet P., Fortier M.A. (1997): Cellular mechanisms involved during oxytocin-induced prostaglandin F2α production in endometrial epithelial cells in vitro: role of cyclooxygenase-2. Endocrinology, 138, 4798-4805. Go to original source... Go to PubMed...
    4. Bishop C.V., Stormshak F. (2006): Nongenomic action of progesterone inhibits oxytocin-induced phosphoinositide hydrolysis and prostaglandin F2α secretion in the ovine endometrium. Endocrinology, 147, 937-942. Go to original source... Go to PubMed...
    5. Bishop C.V., Stormshak F. (2008): Non-genomic actions of progesterone and estrogens in regulating reproductive events in domestic animals. The Veterinary Journal, 176, 270-280. Go to original source... Go to PubMed...
    6. Bogacki M., Silvia W.J., Rękawiecki R., Kotwica J. (2002): Direct inhibitory effect of progesterone on oxytocin-induced secretion of prostaglandin F2α from bovine endometrial tissue. Biology of Reproduction, 67, 184-188. Go to original source... Go to PubMed...
    7. Bramley T. (2003): Non-genomic progesterone receptors in the mammalian ovary: some unresolved issues. Reproduction, 125, 3-15. Go to original source... Go to PubMed...
    8. Burger K., Fahrenholz F., Gimpl G. (1999): Non-genomic effects of progesterone on the signaling function of G protein - coupled receptors. FEBS Letters, 464, 25-29. Go to original source... Go to PubMed...
    9. Dunlap K.A., Stormshak F. (2004): Nongenomic inhibition of oxytocin binding by progesterone in the ovine uterus. Biology of Reproduction, 70, 65-69. Go to original source... Go to PubMed...
    10. Duras M., Mlynarczuk J., Kotwica J. (2005): Non-genomic effect of steroids on oxytocin-stimulated intracellular mobilization of calcium and on prostaglandin F2 and E2 secretion from bovine endometrial cells. Prostaglandins and Other Lipid Mediators, 76, 105-116. Go to original source... Go to PubMed...
    11. Fields M.J., Fields P.A. (1996): Morphological characteristics of the bovine corpus luteum during the estrous cycle and pregnancy. Theriogenology, 45, 1295-1325. Go to original source... Go to PubMed...
    12. Fortier M.A., Krishnaswamy K., Danyod G., BoucherKovalik S., Chapdelaine J.A. (2008): A postgenomic integratred view of prostaglandins in reproduction: implications for other body systems. Journal of Physiology and Pharmacology, 59 (Suppl. 1), 65-89.
    13. Franczak A., Kurowicka B., Oponowicz A., Petroff B.K., Kotwica G. (2006): The effect of progesterone on oxytocin-stimulated intracellular mobilization of Ca2+ and prostaglandin E2 and F2α secretion from porcine myometrial cells. Prostaglandins and Other Lipid Mediators, 81, 37-44. Go to original source... Go to PubMed...
    14. Gimpl G., Fahrenholz F. (2001) The oxytocin receptor system: structure, function, and regulation. Physiological Review, 81, 629-683. Go to original source... Go to PubMed...
    15. Goff A.K. (2004): Steroid hormone modulation of prostaglandin secretion in the ruminant endometrium during the estrous cycle. Biology of Reproduction, 71, 11-16. Go to original source... Go to PubMed...
    16. Grazzini E., Guillon G., Mouilac B., Zingg H.H. (1998): Inhibition of oxytocin receptor function by direct binding of progesterone. Nature, 392, 509-512. Go to original source... Go to PubMed...
    17. Homonics G.E., Silvia W.J. (1988): Effects of progesterone and estradiol-17β on uterine secretion of prostaglandin F2α in response to oxytocin in ovariectomized ewes. Biology of Reproduction, 38, 723-728. Go to original source... Go to PubMed...
    18. Ivell R., Fuchs A.R., Bathgate R., Tillmann G., Kimura T. (2000): Regulation of the oxytocin receptor in bovine reproductive tissues and the role of steroids. Reproduction in Domestic Animals, 35, 134-141. Go to original source...
    19. Kim J.J., Fortier M.A. (1995): Cell type specificity and protein kinase C dependency on the stimulation of prostaglandin E2 and prostaglandin F production by oxytocin and platelet-activating factor in bovine endometrial cells. Journal of Reproduction and Fertility, 103, 239-247. Go to original source... Go to PubMed...
    20. Klein U., Gimpl G., Fahrenholz F. (1995): Alteration of the myometrial plasma membrane cholesterol content with β-cyclodextrin modulates the binding affinity of the oxytocin receptor. Biochemistry, 34, 13784- 13793. Go to original source... Go to PubMed...
    21. Kowalik M., Kotwica J. (2007): Non-genomic effect of ovarian steroids on oxytocin-stimulated prostaglandin (PG)F2α and E2 secretion from bovine endometrial cells. Bulletin of the Veterinary Institute in Pulawy, 51, 37-42.
    22. Kowalik M.K., Kotwica J. (2008): Progesterone receptor membrane component 1 (PGRMC1) gene expression in corpus luteum during estrous cycle in cows. Reproductive Biology, 8, 291-297. Go to original source... Go to PubMed...
    23. Losel R.M., Besong D., Peluso J.J., Wehling M. (2008): Progesterone receptor membrane component 1 - Many tasks for a versatile protein. Steroids, 73, 929-934. Go to original source... Go to PubMed...
    24. Madore E., Harvey N., Parent J., Chapdelaine P., Arosh J.A., Fortier M.A. (2003): An aldose reductase with 20α-hydroxysteroid dehydrogenase activity is most likely the enzyme responsible for the production of prostaglandin F2α in the bovine endometrium. Journal of Biological Chemistry, 278, 11205-11212. Go to original source... Go to PubMed...
    25. McCracken J.A., Custer E.E., Lamsa J.C. (1999): Luteolysis: a nuroendocrine mediated event. Physiological Review, 79, 263-323. Go to original source... Go to PubMed...
    26. Murakami M., Nakatani Y., Tanioka T., Kudo I. (2002): Prostaglandin E synthase. Prostaglandins and Other Lipid Mediators, 68-69, 383-399. Go to original source... Go to PubMed...
    27. Niswender G.D., Juengel J.L., Silvia P.J., Rollyson M.K., McIntush E.W. (2000): Mechanism controlling the function and life span of the corpus luteum. Physiological Review, 80, 1-29. Go to original source... Go to PubMed...
    28. Parent J., Chapdelaine P., Sirois J., Fortier M.A. (2002): Expression of microsomal prostaglandin E synthase in bovine endometrium: coexpression with cyclooxygenase type 2 and regulation by interferon-τ. Endocrinology, 143, 2936-2943. Go to original source... Go to PubMed...
    29. Parent J., Villeneuve C., Fortier M.A. (2003): Evaluation of the contribution of cyclooxygenase 1 and cyclooxygenase 2 to the production of PGE2 and PGF2α in epithelial cells from bovine endometrium. Reproduction, 126, 539-547. Go to original source... Go to PubMed...
    30. Peluso J.J. (2006): Multiplicity of progesterone's actions and receptors in the mammalian ovary. Biology of Reproductive, 75, 2-8. Go to original source... Go to PubMed...
    31. Raw R.E., Silvia W.J., Curry T.E. (1996): Effects of progesterone and estradiol on prostaglandin endoperoxide synthase in ovine endometrial tissue. Animal Reproduction Science, 40, 17-30. Go to original source...
    32. Rekawiecki R., Kotwica J. (2007): Molecular regulation of progesterone synthesis in the bovine corpus luteum. Veterinarni Medicina, 52, 405-412. Go to original source...
    33. Rekawiecki R., Kowalik M.K., Slonina D., Kotwica J. (2008): Regulation of progesterone synthesis and action in bovine corpus luteum. Journal of Physiology and Pharmacology, 59 (Suppl. 9), 75-89.
    34. Salomonsen L.A., Hampton A.L., Clemens J.A., Findlay J.K. (1990): Regulation of gene expression and cellular localization of prostaglandin synthase by oestrogen and progesterone in ovine uterus. Journal of Reproduction and Fertility, 92, 393-406. Go to original source... Go to PubMed...
    35. Skarzynski D.J., Bogacki M., Kotwica J. (1999): Involvement of ovarian steroids in basal and oxytocin-stimulated prostaglandin (PG)F2 secretion by the bovine endometrium in vitro. Theriogenology, 52, 385-397. Go to original source... Go to PubMed...
    36. Xiao C.W., Liu J.M., Sirois J., Goff A.K. (1998): Regulation of cyclooxygenase-2 and prostaglandin F synthase gene expression by steroid hormones and interferont in bovine uterine endometrial cells. Endocrinology, 139, 2293-2299. Go to original source... Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content